<scp>RHD</scp>*weak partial 4.0 is associated with an altered <scp>RHCE</scp>*ce(<scp>48C</scp>, <scp>105T</scp>, <scp>733G</scp>, <scp>744C</scp>, <scp>1025T</scp>) allele in the Tunisian population

Ouchari, Mouna; Polin, Helene; Romdhane, H. Ben; Abdelkefi, S.; Houissa, B.; Chakroun, Tahar; Gabriel, Christian; Hmida, Slama; Yacoub, S.

doi:10.1111/tme.12037

Cited by 7 publications

(23 citation statements)

References 33 publications

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“…The weak D Type 4.0 was the most prevalent molecular weak D type in Tunisians, confirming our previously published data . Comparing our current data (Table ), we concluded that only approximately 69% of all Tunisian donors expressing the weak D Type 4.0 phenotype (53 of a calculated 77) were actually typed as having a serologic weak D phenotype.…”

Section: Resultssupporting

confidence: 90%

“…To a smaller extent, this caveat also applies to alleles of the weak D Type 4 cluster. For instance, the frequency of weak D Type 4.0 is actually 1:105, as determined by PCR in random donors …”

Section: Resultsmentioning

confidence: 99%

“… Among 1000 random donors, including D– donors and representing 2000 RH haplotypes, a total of 19 donors were found carrying a weak D Type 4.0 allele …”

Section: Resultsmentioning

confidence: 99%

“…The RHCE*ce(48C, 105T, 733G, 744C, 1025T) , as observed in Tunisia and France, differs by two silent SNPs from the ceTI Type 2 . Per ISBT Working Group on Red Cell Immunogenetics and Blood Group Terminology, alternative names for this RHCE allele are ceTI Type 2.01, RHCE*01.20.04.01, RHCE*ce.20.04.01, and RHCE*ceVS.04.01 .…”

Section: Methodsmentioning

confidence: 93%

“…Here we identified all samples with a serologic weak D phenotype in a cohort of 13,431 Tunisian blood donations, sequenced their RHD genes, and established the RH haplotypes. The study was designed to obtain data on weak D Type 4.0 in a population known to harbor the greatest prevalence of such allele worldwide . Recently, a work group identified the need for more data for weak D Types 4.0 or 4.1, the topic of this research.…”

Section: Discussionmentioning

confidence: 99%

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Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

et al. 2017

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Background With more than 460 RHD alleles, this gene is the most complex and polymorphic among all blood group systems. The Tunisian population has the largest known prevalence of weak D type 4.0 alleles, occurring in 1 of 105 RH haplotypes. We aimed to establish a rationale for the transfusion strategy of weak D type 4.0 in Tunisia. Study design and methods Donors were randomly screened for the serological weak D phenotype. The RHD coding sequence and parts of the introns were sequenced. To establish the RH haplotype, the RHCE gene was tested for characteristic single nucleotide positions. Results We determined all RHD alleles and the RH haplotypes coding for the serologic weak D phenotype among 13,431 Tunisian donations. A serologic weak D phenotype was found in 67 individuals (0.50%). Among them, 60 carried a weak D type 4 allele: 53 weak D type 4.0, 6 weak D type 4.2.2 (DAR), and 1 weak D type 4.1. Another 4 donors had 1 variant allele each: DVII, weak D type 1, weak D type 3, and weak D type 100, while 3 donors showed a normal RHD sequence. The weak D type 4.0 was most often linked to RHCE*ceVS.04.01, weak D type 4.2.2 to RHCE*ceAR, and weak D type 4.1 to RHCE*ceVS.02, while the other RHD alleles were linked to one of the common RHCE alleles. Conclusions Among the weak D phenotypes in Tunisia, no novel RHD allele was found and almost 90% were caused by alleles of the weak D type 4 cluster, of which 88% represented the weak D type 4.0 allele. Based on established RH haplotypes for variant RHD and RHCE alleles and the lack of adverse clinical reports, we recommend D positive transfusions for patients with weak D type 4.0 in Tunisia.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

“… Among 1000 random donors, including D– donors and representing 2000 RH haplotypes, a total of 19 donors were found carrying a weak D Type 4.0 allele …”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

et al. 2017

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High prevalence of serological weak D phenotype and preponderance of weak D type 4.0.1. genetic variant in a Nigerian population: implications for transfusion practice in a resource-limited setting

Maryam

Mukhtar

Yusuf

et al. 2022

Hematology, Transfusion and Cell Therapy

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RHD genotyping and its implication in transfusion practice

Sassi

Ouchari

Houissa

et al. 2014

Transfusion and Apheresis Science

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*RHD***weak partial 4.0* is associated with an altered RHCEce(48C, 105T, 733G, 744C, 1025T)* allele in the Tunisian population

Abstract: The RHDweak partial 4.0 allele syn. RHD09.03 was estimated to occur 1 in 47 among D+ Tunisians. There was no evidence for other RHD alleles included in the weak D type 4 cluster.

Cited by 7 publications

References 33 publications

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

High prevalence of serological weak D phenotype and preponderance of weak D type 4.0.1. genetic variant in a Nigerian population: implications for transfusion practice in a resource-limited setting

RHD genotyping and its implication in transfusion practice

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RHD*weak partial 4.0 is associated with an altered RHCE*ce(48C, 105T, 733G, 744C, 1025T) allele in the Tunisian population

Abstract: The RHD*weak partial 4.0 allele syn. RHD*09.03 was estimated to occur 1 in 47 among D+ Tunisians. There was no evidence for other RHD alleles included in the weak D type 4 cluster.

Cited by 7 publications

References 33 publications

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

Transfusion strategy for weak D Type 4.0 based on RHD alleles and RH haplotypes in Tunisia

High prevalence of serological weak D phenotype and preponderance of weak D type 4.0.1. genetic variant in a Nigerian population: implications for transfusion practice in a resource-limited setting

RHD genotyping and its implication in transfusion practice

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Product

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*RHD***weak partial 4.0* is associated with an altered RHCEce(48C, 105T, 733G, 744C, 1025T)* allele in the Tunisian population

Abstract: The RHDweak partial 4.0 allele syn. RHD09.03 was estimated to occur 1 in 47 among D+ Tunisians. There was no evidence for other RHD alleles included in the weak D type 4 cluster.