<scp>I</scp>mproving the sensitivity in chiral capillary electrophoresis

Sánchez-López, Elena; Marina, Marı́a Luisa; Crego, Antonio L.

doi:10.1002/elps.201500315

Cited by 31 publications

(16 citation statements)

References 91 publications

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“…The SEFs for d ‐ and l ‐Leu peaks were 2970 and 2400, respectively. To our knowledge, the obtained SEFs were higher than those in the previous reports on the MCE analysis of chiral compounds . As shown in Fig.…”

Section: Resultscontrasting

confidence: 61%

Combination of large‐volume sample stacking with an electroosmotic flow pump with field‐amplified sample injection on cross‐channel chips

et al. 2017

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A combination of two online sample concentration techniques, large‐volume sample stacking with an electroosmotic flow (EOF) pump (LVSEP) and field‐amplified sample injection (FASI), was investigated in microchip electrophoresis (MCE) to achieve highly sensitive analysis. By applying reversed‐polarity voltages on a cross‐channel microchip, anionic analytes injected throughout a microchannel were first concentrated on the basis of LVSEP, followed by the electrokinetic stacking injection of the analytes from a sample reservoir by the FASI mechanism. As well as the voltage application, a pressure was also applied to the sample reservoir in LVSEP‐FASI. The applied pressure generated a counter‐flow against the EOF to reduce the migration velocity of the stacked analytes, especially around the cross section of the microchannel, which facilitated the FASI concentration. At the hydrodynamic pressure of 15 Pa, 4520‐fold sensitivity increase was obtained in the LVSEP‐FASI analysis of a standard dye, which was 33‐times higher than that obtained with a normal LVSEP. Furthermore, the use of the sharper channel was effective for enhancing the sensitivity, e.g., 29 100‐fold sensitivity increase was achieved with the 75‐μm wide channel. The developed method was applied to the chiral analysis of amino acids in MCE, resulting in the sensitivity enhancement factor of 2920 for the separated d‐leucine.

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Section: Resultscontrasting

confidence: 61%

Combination of large‐volume sample stacking with an electroosmotic flow pump with field‐amplified sample injection on cross‐channel chips

et al. 2017

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“…Sanchez‐Lopez et al. reported in their review article that Ma et al. used field‐amplified sample stacking coupled with matrix removal by electroosmotic flow pump, and the authors found a sensitivity enhancement up to 3000 times in chiral analysis when compared to hydrodynamically injected β ‐blockers .…”

Section: Resultsmentioning

confidence: 99%

Approaching over 10 000‐fold sensitivity increase in chiral capillary electrophoresis: Cation‐selective exhaustive injection and sweeping cyclodextrin‐modified micellar electrokinetic chromatography

et al. 2016

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A novel and simple method that combines an online concentration technique with an enantioseparation technique for capillary electrophoresis-namely, cation-selective exhaustive injection and sweeping cyclodextrin-modified micellar electrokinetic chromatography (CSEI-sweeping CD-modified MEKC)-realizes the effective enantioseparation of cationic analytes while keeping a significant increase of detection sensitivity. This technique consists of a slight modification of the basic CSEI-sweeping MEKC. The main idea is to simply add an anionic CD as a chiral selector into the micellar buffer including sodium dodecyl sulfate, but not to change any other buffers in order to preserve the online concentration mechanism. When applied to analysis of the street drug, methamphetamine, the method achieved not only a baseline enantioseparation but also limits of detection (LODs; S/N = 3) of 70-90 pg/mL (ppt) for each isomer. This translates to a more than 10 000-fold improvement compared to the LODs by the usual injection method. The present technique, which was made from a slight modification of CSEI-sweeping MEKC, would give an attractive approach that is applicable to almost any analytes for which CSEI-sweeping MEKC is applicable; all that is required is the selection of an appropriate anionic CD to be added to the micellar buffer.

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“…CE is an effective technique for stereoisomer analysis due to the high separation efficiency. This is also reflected in the number of recent reviews published on CE enantioseparations . The scope of chiral CE‐MS as well as the application of nonaqueous CE in chiral analysis have been addressed.…”

Section: Separation and Analysis Of Small Moleculesmentioning

confidence: 99%

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

et al. 2016

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This review updates and follows-up a previous review by highlighting recent advancements regarding capillary electromigration methodologies and applications in pharmaceutical analysis. General approaches such as quality by design as well as sample injection methods and detection sensitivity are discussed. The separation and analysis of drug-related substances, chiral CE, and chiral CE-MS in addition to the determination of physicochemical constants are addressed. The advantages of applying affinity capillary electrophoresis in studying receptor-ligand interactions are highlighted. Finally, current aspects related to the analysis of biopharmaceuticals are reviewed. The present review covers the literature between January 2013 and December 2015.

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Improving the sensitivity in chiral capillary electrophoresis

Cited by 31 publications

References 91 publications

Combination of large‐volume sample stacking with an electroosmotic flow pump with field‐amplified sample injection on cross‐channel chips

Combination of large‐volume sample stacking with an electroosmotic flow pump with field‐amplified sample injection on cross‐channel chips

Approaching over 10 000‐fold sensitivity increase in chiral capillary electrophoresis: Cation‐selective exhaustive injection and sweeping cyclodextrin‐modified micellar electrokinetic chromatography

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis (2013–2015)

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