<scp><i>Ta</i>EIL1</scp>, a wheat homologue of <scp><i>At</i>EIN3</scp>, acts as a negative regulator in the wheat–stripe rust fungus interaction

Duan, Xiyu; Wang, Xiaojie; Fù, Yànpíng; Tang, Chunlei; Li, Xiaorui; Cheng, Yulin; Hu, Feng; Huang, Lili; Kang, Zhensheng

doi:10.1111/mpp.12044

Cited by 35 publications

(22 citation statements)

References 66 publications

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“…Sugar consistently plays an important role between pathogen and host interactions. Some previous studies indicated that sucrose accumulation was observed in Pst infected wheat as well as in barley infected by powdery mildew [23,24]. In the present study, we found that proline and soluble sugar significantly increased in both the CYR32-infected and V26-infected CM42 leaves, which suggests that proline and sugar is involved in the response to Pst infection in wheat.…”

Section: Discussionsupporting

confidence: 75%

Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat

Chen

Mao

et al. 2019

IJMS

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Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is the most destructive wheat disease and a major problem for the productivity of wheat in the world. To obtain a better understanding about different effects of redox homeostasis and photosystem (PS) to Pst infection in wheat, we investigated the differences in photosynthesis and the antioxidant defense system in wheat cultivar Chuanmai42 (CM42) in response to two Chinese Pst races known as CYR32 and V26. The results showed that V26-infected wheat accumulated a higher reactive oxygen species (ROS), cell death, and energy dissipation than CYR32-infected wheat when compared with the control. Furthermore, we found that the activities of three antioxidant enzymes (APX, GR, and GPX) and four resistance-related enzymes in CYR32-infected wheat were significantly higher than that in V26-infected wheat. In addition, quantitative RT-PCR indicated that the expression levels of two genes associated with resistant stripe rust in CYR32-infected wheat were clearly higher than that in V26-infected wheat. Compared with CYR32-infected wheat, lower photochemical efficiencies were observed in V26-infected wheat at the adult stage. Meanwhile, only a marked decline in D1 protein was observed in V26-infected wheat. We therefore deduced that wheat with stripe rust resistance could maintain high resistance and photosynthetic capacity by regulating the antioxidant system, disease-resistant related enzymes and genes, and the levels of PSII reaction center proteins.

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Section: Discussionsupporting

confidence: 75%

Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat

Chen

Mao

et al. 2019

IJMS

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“…Plants have evolved complex defense mechanisms to recognize and defend against invading pathogens (Pedley and Martin ). These complex response mechanisms are primarily regulated by controlling the expression of pathogen‐responsive genes (Tsuda and Somssich ), generally through activities of transcription factors (Duan et al ). A wide range of transcription factor (TF) families have been identified in plants.…”

Section: Introductionmentioning

confidence: 99%

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust

Wang

Wei

Song

et al. 2018

JIPB

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NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30, from a cDNA library constructed from wheat (Triticum aestivum) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one-hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C-terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst. Silencing of TaNAC30 by virus-induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H O . Finally, we overexpressed TaNAC30 in fission yeast and determined that cell viability was severely reduced in TaNAC30-transformed cells grown on medium containing H O . These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat-Pst interaction.

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“…However, the ET signaling mechanisms during bread wheat-pathogen interactions remain to be clarified. Two recent studies in bread wheat reported that TaEIL1, a wheat homolog of AtEIN3, acts as a negative regulator in the bread wheat-stripe rust fungus interaction (Duan et al, 2013), and the pathogen-induced TaERF1 mediates host responses to both the necrotrophic pathogen Rhizoctonia cerealis and freezing stresses (Zhu et al, 2014).…”

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confidence: 99%

The Wheat Mediator Subunit TaMED25 Interacts with the Transcription Factor TaEIL1 to Negatively Regulate Disease Resistance against Powdery Mildew

et al. 2016

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Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici, is a major limitation for the production of bread wheat (Triticum aestivum). However, to date, the transcriptional regulation of bread wheat defense against powdery mildew remains largely unknown. Here, we report the function and molecular mechanism of the bread wheat Mediator subunit 25 (TaMED25) in regulating the bread wheat immune response signaling pathway. Three homoalleles of TaMED25 from bread wheat were identified and mapped to chromosomes 5A, 5B, and 5D, respectively. We show that knockdown of TaMED25 by barley stripe mosaic virus-induced gene silencing reduced bread wheat susceptibility to the powdery mildew fungus during the compatible plant-pathogen interaction. Moreover, our results indicate that MED25 may play a conserved role in regulating bread wheat and barley (Hordeum vulgare) susceptibility to powdery mildew. Similarly, bread wheat ETHYLENE INSENSITIVE3-LIKE1 (TaEIL1), an ortholog of Arabidopsis (Arabidopsis thaliana) ETHYLENE INSENSITIVE3, negatively regulates bread wheat resistance against powdery mildew. Using various approaches, we demonstrate that the conserved activator-interacting domain of TaMED25 interacts physically with the separate amino-and carboxyl-terminal regions of TaEIL1, contributing to the transcriptional activation activity of TaEIL1. Furthermore, we show that TaMED25 and TaEIL1 synergistically activate ETHYLENE RESPONSE FACTOR1 (TaERF1) transcription to modulate bread wheat basal disease resistance to B. graminis f. sp. tritici by repressing the expression of pathogenesis-related genes and deterring the accumulation of reactive oxygen species. Collectively, we identify the TaMED25-TaEIL1-TaERF1 signaling module as a negative regulator of bread wheat resistance to powdery mildew.

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TaEIL1, a wheat homologue of AtEIN3, acts as a negative regulator in the wheat–stripe rust fungus interaction

Cited by 35 publications

References 66 publications

Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat

Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust

The Wheat Mediator Subunit TaMED25 Interacts with the Transcription Factor TaEIL1 to Negatively Regulate Disease Resistance against Powdery Mildew

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