Tulostoma loonbanglaense: A new species from Azad Jammu and Kashmir using light and scanning electron microscopy and DNA barcoding technique

Abstract

Tulostoma loonbanglaense sp. nov. is described from Azad Jammu and Kashmir, Pakistan. ITS sequences confirm its position within the genus Tulostoma, and suggest that it is separate from other identified species of this genus. The species has globose to subglobose, with wide, solitary and appressed spines basidiospores, the basidiospores are relatively small, 4.39 Â 4.23 μm, that make it distinguished from the related species. Sequences of nr ITS region of the newly described species nested as a distinct taxon in both phylogenetic analyses of the current study.

Diversity of Neotropical stalked-puffball: Two new species of Tulostoma with reticulated spores

“…T . loonbanglaense was described based on two collections, but both from exactly the same location [ 11 ].…”

“…Tulostoma portoricense was described based on two collections, while T. irregulireticulatum was described using a single collection [12,22]. T. loonbanglaense was described based on two collections, but both from exactly the same location [11].…”

“…They also found that some of the characters commonly used to differentiate species are homoplasious or plesiomorphic but suggested that spore ornamentation and endo- and exoperidium characteristics may be good features to delimit species. In addition to the morphological aspects, internal transcribed spacer (ITS) regions between small-subunit and large-subunit ribosomal RNA (rRNA) genes have widely been used in DNA barcoding to identify Tulostoma species [ 8 – 11 ].…”