<scp>IGF</scp>‐1 promotes angiogenesis in endothelial cells/adipose‐derived stem cells co‐culture system with activation of <scp>PI</scp>3K/Akt signal pathway

Lin, Shiyu; Zhang, Qi; Shao, Xin; Zhang, Tao; Xue, Changyue; Shi, Sirong; Zhao, Dan; Lin, Yunfeng

doi:10.1111/cpr.12390

Cited by 104 publications

(76 citation statements)

References 47 publications

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“…Furthermore, IGFs also increase the number and function of EPCs, which may contribute to protection from atherosclerosis, as well as promoting angiogenesis (Bach, 2014). Moreover, it was proven that in endothelial cells, IGF-1 stimulates the expression of angiogenesis-related growth factors with the activation of the PI3-kinase/Akt signaling pathway (S. Lin et al, 2017).…”

Section: R a F Tmentioning

confidence: 99%

Sitagliptin protects diabetic rats with acute myocardial infarction through induction of angiogenesis: role of IGF-1 and VEGF

Khodeer

Bilasy

Farag

et al. 2019

Can. J. Physiol. Pharmacol.

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Angiogenesis is regulated in a tissue-specific manner in all patients, especially those with diabetes. In this study, we describe a novel molecular pathway of angiogenesis regulation in diabetic rats with myocardial infarction (MI) and examine the cardioprotective effects of different doses of sitagliptin. Male rats were divided into 5 groups: normal vehicle group, diabetic group, diabetic + MI, diabetic + MI + 5 mg/kg sitagliptin, and diabetic + MI + 10 mg/kg sitagliptin. Isoproterenol in diabetic rats resulted in significant (p < 0.05) disturbance to the electrocardiogram, cardiac histopathological manifestations, and an increase in inflammatory markers compared with the vehicle and diabetic groups. Treatment with sitagliptin improved the electrocardiogram and histopathological sections, upregulated vascular endothelial growth factor (VEGF) and transmembrane phosphoglycoprotein protein (CD34) in cardiac tissues, and increased serum insulin-like growth factor 1 (IGF-1) and decreased cardiac tissue homogenate for interleukin 6 (IL-6) and cyclooxygenase 2 (COX-2). A relationship was found between serum IGF-1 and cardiac VEGF and CD34 accompanied by an improvement in cardiac function of diabetic rats with MI. Therefore, the observed effects of sitagliptin occurred at least partly through an improvement in angiogenesis and the mitigation of inflammation. Consequently, these data suggest that sitagliptin may contribute, in a dose-dependent manner, to protection against acute MI in diabetic individuals.

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Section: R a F Tmentioning

confidence: 99%

Sitagliptin protects diabetic rats with acute myocardial infarction through induction of angiogenesis: role of IGF-1 and VEGF

Khodeer

Bilasy

Farag

et al. 2019

Can. J. Physiol. Pharmacol.

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“…The mechanisms of interaction and the role of IGF1 in cell and tissue metabolism are still being explored. Numerous IGF1 functions have demonstrated their effects on cell proliferation and differentiation, and on angiogenesis, which facilitates the overgrowth of the implant [ 20 ]. The clinical observations and animal data suggest that the essential pool of IGF1 in the bone matrix may not be sufficiently available for new bone formation during the aging process and in disease states.…”

Section: Introductionmentioning

confidence: 99%

Biological Properties of Low-Toxic PLGA and PLGA/PHB Fibrous Nanocomposite Scaffolds for Osseous Tissue Regeneration. Evaluation of Potential Bioactivity

et al. 2017

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The aim of the study was to evaluate the biocompatibility and bioactivity of two new prototype implants for bone tissue regeneration made from biodegradable fibrous materials. The first is a newly developed poly(l-lactide-co-glycolide), (PLGA), and the second is a blend of PLGA with synthetic poly([R,S]-3-hydroxybutyrate) (PLGA/PHB). The implant prototypes comprise PLGA or PLGA/PHB nonwoven fabrics with designed pore structures to create the best conditions for cell proliferation. The bioactivity of the proposed implants was enhanced by introducing a hydroxyapatite material and a biologically active agent, namely, growth factor IGF1, encapsulated in calcium alginate microspheres. To assess the biocompatibility and bioactivity, allergenic tests and an assessment of the local reaction of bone tissue after implantation were performed. Comparative studies of local tissue response after implantation into trochanters for a period of 12 months were performed on New Zealand rabbits. Based on the results of the in vivo evaluation of the allergenic effects and the local tissue reaction 12 months after implantation, it was concluded that the two implant prototypes, PLGA + IGF1 and PLGA/PHB + IGF1, were characterized by high biocompatibility with the soft and bone tissues of the tested animals.

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“…Further, KEGG pathway data indicated that PI3K/AKT signaling pathway molecules were signi cantly enriched, suggesting that the PI3K/AKP signaling pathway may play an important role during anal stula healing. S Lin et al found that insulin-like growth factor 1 (IGF-1) can promote endothelial cells/fat by activating the PI3K/AKT signaling pathway by observing vascular formation in stem cell co-culture systems [22]. H Zhou reports that the PI3K/AKT signaling pathway promotes paracrine functions of ASCs [23].…”

Section: Discussionmentioning

confidence: 99%

The Efficacy and Mechanism of Autologous Fat Stem Cells Combined with Microcarrier 6 Transplantation for Anal Fistula Treatment

Fan

et al. 2020

Preprint

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Background: Adipose-derived stem cells (ASCs) function in multi-directional differentiation, proliferation, and tissue regeneration. It is not clear whether microcarrier 6 can promote the migration, differentiation, and regeneration of fat stem cells, and improve therapeutic effects for the treatment of anal fistula.Methods: Japanese big ear rabbits were employed to establish an anal fistula model, and ASC-microcarrier 6 mixed transplantation was used as a treatment. HE staining, immunohistochemistry, and RNA sequencing were used to observe the effect of ASC-microcarrier 6 transplantation on anal fistula healing, in comparison with the ASC treatment group, rubber band operation group, and control group.Results: HE staining indicated scattered striated muscle cells and epithelial tissues in fistula tissues of the ASC-microcarrier 6 complex group and the ASC treatment group, while a small number of lymphocytes were clustered around the microcarrier 6, and fat cell aggregation was seen in the ASC treatment group. RNA sequence analysis showed that differential genes were mainly concentrated in striated muscle cells, vascular smooth muscle, and other tissues. PI3K/AKT signaling pathway molecules were significantly enriched, granulation tissue and lymphocyte infiltration were observed in the rubber band string operation group, and a large amount of necrotic tissue was seen in the control group.Conclusion: Microcarrier 6 is beneficial to the multi-directional differentiation of ASCs, which can provide a good environment for the survival of ASCs and promote fistula healing.

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IGF‐1 promotes angiogenesis in endothelial cells/adipose‐derived stem cells co‐culture system with activation of PI3K/Akt signal pathway

Cited by 104 publications

References 47 publications

Sitagliptin protects diabetic rats with acute myocardial infarction through induction of angiogenesis: role of IGF-1 and VEGF

Sitagliptin protects diabetic rats with acute myocardial infarction through induction of angiogenesis: role of IGF-1 and VEGF

Biological Properties of Low-Toxic PLGA and PLGA/PHB Fibrous Nanocomposite Scaffolds for Osseous Tissue Regeneration. Evaluation of Potential Bioactivity

The Efficacy and Mechanism of Autologous Fat Stem Cells Combined with Microcarrier 6 Transplantation for Anal Fistula Treatment

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