<scp>l</scp>‐Arginine ameliorates oxidative stress in alloxan‐induced experimental diabetes mellitus

El-Missiry, Mohamed A; Othman, Azza I.; Amer, Maggie E.

doi:10.1002/jat.952

Cited by 79 publications

(50 citation statements)

References 43 publications

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“…It seems that there is an association between radiationinduced oxidative stress and elevated levels of lipid fractions and LDL (Onody et al, 2003). This association is similarly observed in other conditions characterized by increased oxidative stress (El-Missiry et al, 2004;ZwirskaKorczala et al, 2003). Therefore, this study suggests that additional experiments will be performed taking into consideration the levels of LDL, high density lipoprotein (HDL), lipid peroxidation through MDA, GSH content, catalase (CAT) activity and DNA content.…”

Section: Resultsmentioning

confidence: 68%

The biochemical changes in rats blood serum levels exposed to different gamma radiation doses

Abdelhalim¹,

Moussa²

2013

Afr. J. Pharm. Pharmacol.

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This study aimed to address the different gamma radiations doses effect on the liver and kidney function of rats: in vivo. A total of 60 healthy male Wistar-Kyoto rats were whole body gamma irradiated with Co 60 source with 0.883 cG/sec dose rate at the beginning of the experiment. The rats were randomly divided into 4 gamma-irradiation groups (25, 50, 75 and 100 Gy) The serum levels of activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), UREA, creatinine (CREA) and uric acid (UAC) were measured using automated biochemical analyzer. The ALT, GGT, ALP values significantly decreased with the different gamma radiation doses compared with the control. The AST and UREA values significantly decreased after irradiation with 25 and 50 Gy gamma radiation doses compared with the control while it significantly increased with 75 and 100 Gy gamma radiation doses. The levels of CREA values decreased with no significant manner after the irradiation with gamma radiation doses compared with the control. The levels of UAC values significantly increased with 50, 75 and 100 Gy gamma radiation doses. The serum ALT and AST levels are common markers for hepatic toxicity: A lower amount of ALP indicates liver problems. The decreased CREA and the increased UAC levels might indicate development of nephritis and renal dysfunction. The excess UAC might be converted to crystals depositing in the tiny tubes of the kidney and causing acute kidney damage. It is proposed that oxidative stress is linked to the organ damage following exposure to ionizing radiation, and after the onset of oxidative stress, antioxidant treatment should be applied to delay or prevent the progression of damage.

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Section: Resultsmentioning

confidence: 68%

The biochemical changes in rats blood serum levels exposed to different gamma radiation doses

Abdelhalim¹,

Moussa²

2013

Afr. J. Pharm. Pharmacol.

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“…Citrulline is the precursor of Arg [133]. Studies suggested that Arg can scavenge ROS in rat hearts [134] and abrogate oxidative stress in experimental mice [135,136] and patient [137]. Fujita and Yanaga reported that the levels of Ala, Cit and Pro are 0.258-0.327 mM, 0.019-0.056 mM and 0.124-0.141 mM in human plasma, respectively [12].…”

Section: Effects Of the Combination Of Ala Cit And Pro On • Oh-inducmentioning

confidence: 99%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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a b s t r a c tThe present study explored the apoptosis pathways in hydroxyl radicals ( • OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca 2 þ -free PCS in the presence of 40 μM FeSO 4 /20 μM H 2 O 2 . The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca 2 þ was involved in calpain activation and phosphatidylserine (PS) exposure in • OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca 2 þ was involved in DNA fragmentation in • OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca 2 þ -involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala 10 Pro 4 Cit 1 )] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala 10 Pro 4 Cit 1 effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca 2 þ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in • OH-induced carp erythrocytes. Ala 10 Pro 4 Cit 1 produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian.

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“…As an essential nutrient, it plays an important role in cell division, the healing of wounds, removing ammonia from the body, immune function such as stimulating T-and natural killer cell activity and influencing pro-inflammatory cytokine level, and the release of hormones [1][2][3]. Many studies have been shown that Arg has dual effects as toxic or protective effects against toxicity in the cells [4][5][6]. Arg serves also as an intermediary in the urea cycle and as a precursor for protein, polyamine, creatine and nitric oxide (NO) biosynthesis which is involved in the physiological control of different tissue at lower levels.…”

Section: Introductionmentioning

confidence: 99%

Administering the Optimum Dose of l-Arginine in Regional Tumor Therapy

Moawad

2013

Ind J Clin Biochem

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The purpose of this study is optimizing the Larginine (L-Arg) doses on the basis of chemical structure in regional accessible tumor therapy to settle down a new protocol for the treatment of cancer.3 H-thymidine-based cell proliferation assay was performed in vitro on tumor cell lines of fibrosarcoma (FS), lymphosarcoma-ascitic and on normal cell line of NIH 3T3 after treatment with different concentrations of L-Arg in phosphate buffered saline (PBS). The cultures were harvested after 22 h and the incorporated radioactivity was counted to identify their histologic grades as described in earlier studies. In vivo therapy of murine tumors was conducted where FS cells injected subcutaneously at ventro-lateral position of mice. Various drug delivery schedules were injected into the centre of tumor base, once a day for 4 days. Tumor diameter and survivals were monitored where the day of sacrifice was considered for monitoring the survival period. By identifying the histologic grades of the treated cultures in vitro and in vivo by different concentrations of L-Arg, the corresponding energy of such concentrations were determined. An efficient model with a good fit (R 2 = 0.98) was established to describe the energy yield by L-Arg dose. The equivalence between the tumor histologic grade and energy of the L-Arg dose delivered in saline (PBS) environment is the optimum condition for regional tumor therapy achieves higher survival rate. The selective cytotoxicity to tumor cells with minimal damage to normal cells by L-Arg due to its chemical structure suggests to be considered the most promising drug for regional therapy of the accessible tumors like breast cancers of early stage with no distant metastasis.

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l‐Arginine ameliorates oxidative stress in alloxan‐induced experimental diabetes mellitus

Cited by 79 publications

References 43 publications

The biochemical changes in rats blood serum levels exposed to different gamma radiation doses

The biochemical changes in rats blood serum levels exposed to different gamma radiation doses

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Administering the Optimum Dose of l-Arginine in Regional Tumor Therapy

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