<scp>l</scp>-Methionine Production by Methionine Analog-resistant Mutants OfCorynebacterium glutamicum

Kase, Hiroshi; Nakayama, Kiyoshi

doi:10.1080/00021369.1975.10861582

Cited by 6 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since methionine is a sulphur-containing amino acid, it is expected that more synthesis could occur as the elemental sulphur is supplied to the medium. The use of ammonium sulphate in methionine production have been reported by Kase and Nakayama [8], Anike and Okafor [22]. Pham et al, [4] reported that methionine production is a function of nitrogen concentration.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Fermentation Conditions of Bacillus thuringiensis EC1 for Enhanced Methionine Production

Anakwenze¹,

Ezemba²,

Ekwealor³

2014

AiM

View full text Add to dashboard Cite

Bacillus thuringiensis EC1, isolated from the fermented oil bean seed, Pentachletra macrophila Benthan, produced a methionine yield of 1.89 mg/ml. The influence of cultural conditions on methionine accumulation by B. thuringiensis EC1 showed that a 20% medium/fermenter volume ratio and a 5% inoculum size increased methionine yield. The carbon of choice was maltose and at 8% level stimulated methionine production. Among the nitrogen sources studied, ammonium sulphate was found to be the best and at 1% concentration produced a methionine yield of 2.56 mg/ml. All growth-promoting substances and their mixtures enhanced methionine accumulation by B. thuringiensis EC1. The effect of Vitamins on methionine production showed that riboflavin and thiamine HCl at 1.0 µg/ml yielded 2.49 mg/ml and 2.80 mg/ml methionine respectively. The influence of bivalent metals on methionine accumulation indicated that Zn 2+ at all concentration stimulated methionine production. Mg 2+ and Ba 2+ at 0.1 µg/ml and 10.0 µg/ml respectively improved methionine yield. Optimizing the cultural conditions of B. thuringiensis EC1 in submerged medium gave a methionine yield of 3.18 mg/ml.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Attempts have been made to overproduce biologically active L-methionine using fermentation [8]- [10].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Fermentation Conditions of Bacillus thuringiensis EC1 for Enhanced Methionine Production

Anakwenze¹,

Ezemba²,

Ekwealor³

2014

AiM

View full text Add to dashboard Cite

show abstract

“…Since b is small, the difference between the dilution rates for maximum biomass and methionine production should also be small. (2) and (3). 3).…”

Section: Continuous Production Experimentsmentioning

confidence: 99%

Effect of Dissolved Oxygen on Continuous Production of Methionine

Sharma

Gomes

2001

Eng. Life Sci.

View full text Add to dashboard Cite

Methionine production by a mutant strain of Corynebacterium lilium was studied at different dilution rates and different dissolved oxygen concentrations, based on a statistical central composite design. The effect of dissolved oxygen levels between 2 % and 60 % and dilution rate levels between 0.04 to 0.29 on continuous production of methionine was studied. The process is described by the Leudeking‐Piret model. The experimentally obtained maxima for methionine and biomass productivities, observed at the same dilution rate of 0.17 h–1, also corresponds to the theoretical prediction based on this model. This experimental observation is further supported by the surface response prediction of a dilution rate of 0.16 h–1 for maximum methionine productivity and 0.15 h–1 for cell mass productivity. Also, beyond the critical value of 30 % dissolved oxygen, the apparent benefits of oxygen transfer become less significant.

show abstract

“…Corynebacterium glutamicum is well known for its ability to produce various amino acids [19][20][21][22]. C. glutamicum strains that could produce high level of l-methionine have been reported [23][24][25]. For example, redirecting the carbon flow from l-lysine pathway to l-methionine pathway in C. glutamicum led to 2.9 g/L l-methionine [26].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of methionine adenosyltransferase in Corynebacterium glutamicum for production of S‐adenosyl‐l‐methionine

Han

Wang

2015

Biotech and App Biochem

View full text Add to dashboard Cite

Two genes encoding methionine adenosyltransferase, SAM2 from Saccharomyces cerevisiae and metK from Corynebacterium glutamicum, were individually cloned into pDXW-8, the shuttle vector between Escherichia coli and C. glutamicum, and overexpressed in E. coli DH5α and C. glutamicum ATCC13032. In DH5α, both genes were overexpressed and their protein products showed the activity of methionine adenosyltransferase. In ATCC13032, metK was overexpressed, its product MetK showed the enzyme activity and could convert l-methionine to S-adenosyl-l-methionine (SAM). However, when SAM2 was overexpressed in ATCC13032, neither the enzyme activity nor the conversion of SAM from l-methionine was observed. Reverse transcription PCR analysis and SDS-PAGE showed that SAM2 was transcribed but not translated in C. glutamicum. Therefore, SAM2-C, a mutant SAM2, was constructed by codon optimization, and overexpressed in ATCC13032; it was well transcribed and translated, and could convert l-methionine to SAM. Finally, SAM2-C and metK were individually overexpressed in E. coli BL21(DE3), and their products SAM2-C and MetK were purified and characterized. The optimum activity for both enzymes was found at pH 8.5 and 35 °C; SAM2-C and MetK have similar K for ATP, but quite different K for l-methionine. These results suggest that SAM2-C and MetK can be useful for developing C. glutamicum to produce SAM.

show abstract

l-Methionine Production by Methionine Analog-resistant Mutants OfCorynebacterium glutamicum

Cited by 6 publications

References 3 publications

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Effect of Dissolved Oxygen on Continuous Production of Methionine

Overexpression of methionine adenosyltransferase in Corynebacterium glutamicum for production of S‐adenosyl‐l‐methionine

Contact Info

Product

Resources

About

l-Methionine Production by Methionine Analog-resistant Mutants OfCorynebacterium glutamicum

Cited by 6 publications

References 3 publications

Optimization of Fermentation Conditions of &lt;i&gt;Bacillus thuringiensis&lt;/i&gt; EC1 for Enhanced Methionine Production

Optimization of Fermentation Conditions of &lt;i&gt;Bacillus thuringiensis&lt;/i&gt; EC1 for Enhanced Methionine Production

Effect of Dissolved Oxygen on Continuous Production of Methionine

Overexpression of methionine adenosyltransferase in Corynebacterium glutamicum for production of S‐adenosyl‐l‐methionine

Contact Info

Product

Resources

About

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production

Optimization of Fermentation Conditions of <i>Bacillus thuringiensis</i> EC1 for Enhanced Methionine Production