<scp>l</scp>‐Ornithine and <scp>l</scp>‐lysine stimulate gastrointestinal motility via transient receptor potential vanilloid 1

Nakato, Junya; Ho, Yee Yin; Omae, Ryo; Maekawa, Takafumi; Uchida, Kunitoshi; Tominaga, Makoto; Kim, Minji; Goto, Tsuyoshi; Takahashi, Nobuyuki; Kawada, Teruo; Akiduki, Saori; Kanamoto, Ryuhei; Ohinata, Kousaku

doi:10.1002/mnfr.201700230

Cited by 13 publications

(8 citation statements)

References 44 publications

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“…l -Pipecolic acid can promote intestinal propulsive rate by increasing the levels of 5-hydroxytryptamine and acetylcholine in the colon , NCC could improve the contents of DCA, UDCA, CDCA, acetic acid, butyric acid, l -pipecolic acid, l -ornithine, and l -lysine through improving the relative abundance of Lactobacillus , Prevotellaceae_UCG-001 , and Anaerotruncus . Finally, NCC improved intestinal motility, and constipation was cured.…”

Section: Resultsmentioning

confidence: 99%

“…80 L-Ornithine and L-lysine can enhance intestinal motility by stimulating intestinal secretion through transient receptor potential vanilloid 1, muscarinic acetylcholine receptor, and NO synthase. 90,91 NCC could improve the contents of DCA, UDCA, CDCA, acetic acid, butyric acid, L-pipecolic acid, L-ornithine, and L-lysine through improving the relative abundance of Lactobacillus, Prevotella-ceae_UCG-001, and Anaerotruncus. Finally, NCC improved intestinal motility, and constipation was cured.…”

Section: Resultsmentioning

confidence: 99%

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Nanocrystalline Cellulose Cures Constipation via Gut Microbiota Metabolism

et al. 2022

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Constipation can seriously affect the quality of life and increase the risk of colorectal cancer. The present strategies for constipation therapy have adverse effects, such as causing irreversible intestinal damage and affecting the absorption of nutrients. Nanocrystalline cellulose (NCC), which is from natural plants, has good biocompatibility and high safety. Herein, we used NCC to treat constipation assessed by the black stool, intestinal tissue sections, and serum biomarkers. We studied the effect of NCC on gut microbiota and discussed the correlation of gut microbiota and metabolites. We evaluated the long-term biosafety of NCC. NCC could effectively treat constipation through gut microbiota metabolism, which required a small dosage and did not affect the organs and intestines. NCC could be used as an alternative to medications and dietary fiber for constipation therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Nanocrystalline Cellulose Cures Constipation via Gut Microbiota Metabolism

et al. 2022

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“…Until now, PIPA has rarely been studied as a gut metabolite, and its relationship to the host has been rarely studied as well. A recent study reported that orally taking L -lysine, which is a precursor of PIPA, could improve the small intestine propulsive rate in mice (Nakato et al, 2017). Although it seems that PIPA may be involved in intestinal motility, there is not an abundance of data or direct evidence to support this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut

Chen

Ren

et al. 2019

Front. Microbiol.

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The benefits of probiotics for constipation are widely accepted, but the mechanisms involving gut metabolites are unclear. In this study, we investigated the effects of Lactobacillus casei strain Shirota (LcS) on constipated patients and revealed that a metabolite mediator is involved in the LcS-induced constipation alleviation. Sixteen constipated patients and 22 non-constipated participants were recruited. The subjects consumed 100 mL of an LcS beverage (10 8 CFU/mL) per day for 28 days. The fecal non-volatile metabolites were determined by GC/MS, and the targeted metabolites were further verified in a constipated mouse model. In constipated patients, LcS intervention significantly improved defecation frequency (from 4.81 to 7.81 times per week, p < 0.05), stool consistency (from 2.52 to 3.68, p < 0.05) and constipation-related symptoms. A total of 14 non-volatile fecal metabolites were obtained as potential constipation-related metabolites that were regulated by LcS. Among these metabolites, pipecolinic acid (PIPA) had a significant positive correlation with defecation frequency in constipated patients. PIPA significantly promoted the small intestinal propulsive rate (from 25.45 to 39.68%) and increased the number of fecal pellets (from 30.38 to 57.38 pellets) in constipated mice ( p < 0.05). The 5-hydroxytryptamine (5-HT) and acetylcholine (ACh) in colonic tissue may be partly involved in PIPA-mediated constipation alleviation. In conclusion, PIPA was a metabolic mediator in the gut that participated in LcS-induced constipation alleviation.

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“…Glutamate can be converted into N -acetylglutamate through catalysis, which then participates in arginine synthesis as a prerequisite for N -acetylornithine [ 30 ]. A study found that L -ornithine stimulated, but conversely, arginine suppressed, gastrointestinal motility after oral administration in mice [ 31 ]. Another cross-sectional study showed that levels of serum metabolites of N -acetyl- L -glutamic acid were significantly lower in constipated women of reproductive age than in healthy controls [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

Wang

et al. 2023

Nutrients

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Functional constipation (FC) is a gastrointestinal disorder with high incidence, and it seriously affects patients’ physical and mental health. Several studies have shown that the gut microbiome is associated with FC, but these studies have produced inconsistent findings, with few reflecting the relationship between the gut microbiome and metabolites. This study used 16S rRNA microbial genomics and non-target metabolome based on liquid chromatography–mass spectrometry to analyze the gut microbiota composition and serum metabolic profiles of 30 FC patients and 28 healthy individuals. We found that patients with FC and healthy individuals have different gut microbiota structures and serum metabolic profiles. FC patients had more Bacteroides and butyrate-producing bacteria (Roseburia, Faecaliberium, Butyriccoccus). The upstream products of host arginine biosynthesis (2-oxoglutaric acid, L-glutamic acid, N-acetylornithine, and L-ornithine) were significantly reduced in FC patients’ serum metabolites. In summary, our study describes the gut microbiome and serum metabolome of patients with functional constipation. It reveals that functional constipation may be associated with increased Bacteroidetes and downregulation of upstream products of host arginine biosynthesis, which may be potential markers for diagnosing functional constipation.

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l‐Ornithine and l‐lysine stimulate gastrointestinal motility via transient receptor potential vanilloid 1

Abstract: Orally administered Orn and Lys stimulate GI motility via TRPV1, mAChR and NO synthase in mice.

Cited by 13 publications

References 44 publications

Nanocrystalline Cellulose Cures Constipation via Gut Microbiota Metabolism

Nanocrystalline Cellulose Cures Constipation via Gut Microbiota Metabolism

Lactobacillus casei Strain Shirota Alleviates Constipation in Adults by Increasing the Pipecolinic Acid Level in the Gut

Characteristics of the Gut Microbiome and Serum Metabolome in Patients with Functional Constipation

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