<scp>PTH</scp>/<scp>SDF</scp>‐1α cotherapy promotes proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells

Du, Liqing; Feng, Ruijuan; Ge, Shaohua

doi:10.1111/cpr.12286

Cited by 37 publications

(38 citation statements)

References 48 publications

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“…In the present study, we discovered that the expression of Runx2 was decreased post-MP treatment, whereas it was upregulated after the transplantation of SDF-1α-GFP-BMSCs, which is consistent with the results of previous studies [52, 53]. Lingqian et al reported that the cotherapy with the parathyroid hormone (PTH) and SDF-1 promoted osteogenic differentiation of human periodontal ligament stem cells by enhancing OCN expression and ALP activity [54], which is in line with the findings of the present study. Osteoblast differentiation is key to bone formation, in which bone morphogenetic protein-2/Smad signaling pathway is involved [55].…”

Section: Discussionsupporting

confidence: 91%

Stromal-Cell-Derived Factor (SDF) 1-Alpha Overexpression Promotes Bone Regeneration by Osteogenesis and Angiogenesis in Osteonecrosis of the Femoral Head

Yang

Xue

Guan

et al. 2018

Cell Physiol Biochem

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Background/Aims: Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease. Previous studies suggested that stromal-cell-derived factor (SDF)-1 was involved in osteogenesis and angiogenesis. However, whether SDF-1 potentiates the angiogenesis and osteogenesis of bone marrow-derived stromal stem cells (BMSCs) in ONFH is not clear. Methods: BMSCs were transfected with green fluorescent protein (GFP) or the fusion gene encoding GFP and SDF-1α, and transgenic efficacy was monitored by immunofluorescence. The expression of SDF-1α, runt-related transcription factor 2 (Runx2, osteocalcin (OCN), and alkaline phosphatase (ALP) at the mRNA level was measured by real-time polymerase chain reactions (RT-PCR). The expression of SDF-1α, Runx2, OCN, and p-Smad1/5 were measured at the protein level by Western blot. Transwell migration assay and tube formation assay were utilized to detect the angiogenesis in vitro, whereas the in vivo angiogenesis was monitored by angiography. Immunohistological staining and micro-CT scanning were conducted to assess the histological changes in morphology. Results: In vitro, SDF-1α overexpression in BMSCs promoted osteogenic differentiation and upregulated the expression of osteogenic-related proteins, such as ALP, Runx2, OCN, and p-Smadl/5. In the methylprednisolone induced ONFH rat model used in our investigation, the overexpression of SDF-1α in BMSCs promoted significantly more bone regeneration and the expression of OCN and Runx2 as compared with the effect of vehicle overexpression. Moreover, the morphology of ONFH was ameliorated after the transplantation of BMSCs with SDF-1α overexpression. Furthermore, SDF-1α overexpression in BMSCs significantly increased osteoblastic angiogenesis as indicated by the increased tube formation ability, CD31 expression, and vessel volume. Conclusion: SDF-1α overexpression in BMSCs promotes bone generation as indicated by osteogenesis and angiogenesis, suggesting SDF-1α may serve as a therapeutic drug target for ONFH treatment.

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Section: Discussionsupporting

confidence: 91%

Stromal-Cell-Derived Factor (SDF) 1-Alpha Overexpression Promotes Bone Regeneration by Osteogenesis and Angiogenesis in Osteonecrosis of the Femoral Head

Yang

Xue

Guan

et al. 2018

Cell Physiol Biochem

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“…39 As displayed in Figure 4B, the number of calcium nodules in the experiment group exposed to TDNs for 2 weeks was remarkably higher than that in the blank group without exposure to TDNs ( Figure 4B). 39 As displayed in Figure 4B, the number of calcium nodules in the experiment group exposed to TDNs for 2 weeks was remarkably higher than that in the blank group without exposure to TDNs ( Figure 4B).…”

Section: Enhancement Of Calcium Nodule Formation and Alp Activitymentioning

confidence: 81%

Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

et al. 2019

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Objective To explore the effects and underlying biological mechanisms of tetrahedral DNA nanostructures (TDNs) on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods Real‐time cell analysis (RTCA) and CCK8 were used to screen the best concentration of TDN for PDLSCs. Cell proliferation and osteogenic differentiation were assessed after PDLSCs were treated with TDN. Data were analysed using one‐way ANOVA. Results Tetrahedral DNA nanostructures could play a crucial role in accelerating the proliferation of PDLSCs and had the strongest promotive effect on PDLSCs at a concentration of 250 nmol/L. Simultaneously, the osteogenic differentiation of PDLSCs could be promoted significantly by TDNs and the finding displayed that the Wnt/β‐catenin signalling pathway might be the underlying biological mechanisms of TDNs on promoting the osteogenic differentiation of PDLSCs. Conclusion Tetrahedral DNA nanostructure treatment facilitated the proliferation of PDLSCs, significantly promoted osteogenic differentiation by regulating the Wnt/β‐catenin signalling pathway. Therefore, TDNs could be a novel nanomaterial with great potential for application to PDLSC‐based bone tissue engineering.

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“…The extracted teeth were transported from the clinic to the laboratory in Dulbecco's modified Eagle's medium (DMEM, Hyclone, Logan, UT, USA) with 5% antibiotics (100 U/mL of penicillin, 100 mg/mL of streptomycin, Sigma‐Aldrich, St Louis, MO, USA). PDL tissue from the middle third of the root surface was collected, and single cell of PDLSCs was acquired according to our previous study . PDL tissue was cut into small pieces and digested with 3 mg/mL collagenase I (Sigma‐Aldrich) and 4 mg/mL dispase II (Invitrogen, Carlsbad, CA, USA) for 40 minutes at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…PDL tissue from the middle third of the root surface was collected, and single cell of PDLSCs was acquired according to our previous study. 33 In brief, cells were incubated with DMEM containing 10 μL test reagents for 2.5 hours at 37°C. The absorbance was measured at a wavelength of 450 nm with a microplate reader (SPECTROstar Nano, BMG Labtech, Offenburg, Germany).…”

Section: Cell Isolation and Cultivationmentioning

confidence: 99%

Sequential application of bFGF and BMP‐2 facilitates osteogenic differentiation of human periodontal ligament stem cells

Kang

Liang

et al. 2019

J of Periodontal Research

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Background and Objectives Basic fibroblast growth factor (bFGF) promotes cells proliferation and chemotaxis and maintains stemness while inhibits mineralized nodule formation. Bone morphogenetic protein 2 (BMP‐2) shows great potential in promoting bone formation. However, sequential application of these two growth factors on periodontal ligament stem cells (PDLSCs) has not been explored. In this study, we aimed to identify the optimal concentration and time of bFGF on PDLSCs proliferation, migration and then investigate the sequential delivery of bFGF and BMP‐2 on osteogenic differentiation of PDLSCs in vitro. Materials and Methods Periodontal ligament stem cells were isolated by limiting dilution method. Dose‐dependent additive effects of bFGF and BMP‐2 on PDLSCs were detected. Cell counting assay, cell migration assay, alkaline phosphatase (ALP) activity assay, Alizarin red staining, quantitative real‐time polymerase chain reaction (qRT‐PCR), and western blot analysis were used to determine different application modalities of bFGF and BMP‐2 on proliferation, migration, and osteogenic differentiation of PDLSCs. Results 50 ng/mL bFGF significantly promoted PDLSCs proliferation and chemotaxis while time‐dependently inhibited BMP‐2 induced ALP activity. Sequential application of 25 ng/mL bFGF for first 3 days and followed with 50 ng/mL BMP‐2 for another 9, 18, and 25 days significantly promoted PDLSCs osteogenic differentiation. Compared with bFGF and BMP‐2 simultaneous group, sequential application of bFGF and BMP‐2 group significantly enhanced ALP activity, osteogenesis‐related genes and proteins expression and mineral deposition. Conclusion Sequential application of bFGF and BMP‐2 synergistically promoted osteogenic differentiation of PDLSCs, and this sequential application modality of growth factors would provide a new strategy for periodontal regeneration.

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PTH/SDF‐1α cotherapy promotes proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells

Cited by 37 publications

References 48 publications

Stromal-Cell-Derived Factor (SDF) 1-Alpha Overexpression Promotes Bone Regeneration by Osteogenesis and Angiogenesis in Osteonecrosis of the Femoral Head

Stromal-Cell-Derived Factor (SDF) 1-Alpha Overexpression Promotes Bone Regeneration by Osteogenesis and Angiogenesis in Osteonecrosis of the Femoral Head

Effect of tetrahedral DNA nanostructures on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Sequential application of bFGF and BMP‐2 facilitates osteogenic differentiation of human periodontal ligament stem cells

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