<scp>SSR</scp> markers for <i>Filago</i> subg. <i>Filago</i> (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera

Gutiérrez-Larruscain, David; Malvar-Ferreras, Teresa; Martínez‐Ortega, M. Montserrat; Rico, Enrique; Andrés‐Sánchez, Santiago

doi:10.1002/aps3.1171

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…Some molecular based studies have been successfully carried out on Anaphalis and the Asteraceae Family by using molecular markers derived from nuclear ribosomal DNA (nrDNA), namely the Internal Transcribed Spacer (ITS) region [2]; and External Transcribed Spacers (ETS) [5]; and some use Simple Sequence Repeat (SSR) [12], [13]. The ITS area has proven to be a useful source of molecular information for genetic variation and phylogenetic studies in the Angiosperms plant group [14]; and RAPD marker for phylogenetic analysis [15], [16].…”

Section: Introductionmentioning

confidence: 99%

The Detection of Anaphalis spp. Genetic Diversity Based on Molecular Character (using ITS, ETS, and EST-SSR markers)

Ade

Hakim

Arumingtyas

et al. 2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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Anaphalis is the natural vegetation component in mountainous areas and is found in volcanic soils. Anaphalis is one of the species which existence is currently presumed to decrease and is feared to be extinct in nature. The purpose of this study was to detect genetic diversity of Anaphalis spp. in Bromo Tengger Semeru National Park (BTSNP) based on molecular characters by using ITS, ETS, and EST-SSR markers in supporting the conservation aspects of Anaphalis genetically. This research method was carried out by exploring the existence of Anaphalis populations, their coordinates marked by GPS, and leaf samples collected as a source of molecular analysis material. PCR products from ITS and ETS markers were sequenced, and the results' phylogenetic were analyzed using the MEGA6 program. PCR products from EST-SSR markers were performed by scoring DNA bands (allel) and both variations and genetics population were analyzed by using the POPGENE 1.32 program. Anaphalis populations found in BTSNP are Anaphalis javanica, A. longifolia, and A. viscida. The genetic diversity of Anaphalis spp. in BTSNP has polymorphism potential that is high enough, which is 57% (ITS) and 31% (ETS-partial), with a maximum likelihood phylogenetic tree topology which is monophyletic separated into four clusters consisting one cluster outgroup and three others being an ingroup. Whereas based on the genetic diversity value of the EST-SSR sequences in Anaphalis spp. in BTSNP shows that only A. longifolia populations in the Ranu Kumbolo area that have a high genetic diversity value (0.024) compared to the other two Anaphalis species. The highest genetic distance of Anaphalis spp. BTSNP in A. longifolia is found in the population of Penanjakan and Mt. Batok areas (0.040) with the smallest gene flow rate (0.428). Further research is needed to obtain a more complete picture of Anaphalis genetic diversity by using more molecular markers, bigger population numbers, more individuals and in bigger populations in other conservation areas in supporting the Anaphalis conservation strategy program.

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Section: Introductionmentioning

confidence: 99%

The Detection of Anaphalis spp. Genetic Diversity Based on Molecular Character (using ITS, ETS, and EST-SSR markers)

Ade

Hakim

Arumingtyas

et al. 2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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SSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera

et al. 2018

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Premise of the StudyMicrosatellite primers were developed for the first time in the genus Filago (Gnaphalieae: Asteraceae). These markers will facilitate low‐scale phylogenetic, phylogeographic, and population genetic studies within the genus Filago.Methods and ResultsTen pairs of polymorphic microsatellite primers (as well as five pairs of monomorphic primers) were identified and optimized on two species of Filago (F. gaditana and F. carpetana) using a microsatellite‐enrichment library method and 454 GS‐FLX technique. The polymorphic primers amplified tri‐ to hexanucleotide repeats and showed one to six alleles per locus for both species. Transferability was performed in 29 samples corresponding to nine representative species of Filago.ConclusionsThe results indicate the utility of the newly developed markers, which will be useful to delve into the phylogenetic relationships among the taxa within Filago. These microsatellites will enable studies of phylogeographic, reproductive, and genetic variation.

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The newly developed single nucleotide polymorphism (SNP) markers for a potentially medicinal plant, Crepidiastrum denticulatum (Asteraceae), inferred from complete chloroplast genome data

et al. 2019

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SSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera

Cited by 4 publications

References 10 publications

The Detection of Anaphalis spp. Genetic Diversity Based on Molecular Character (using ITS, ETS, and EST-SSR markers)

The Detection of Anaphalis spp. Genetic Diversity Based on Molecular Character (using ITS, ETS, and EST-SSR markers)

SSR markers for Filago subg. Filago (Gnaphalieae: Asteraceae) and cross‐amplification in three other subgenera

The newly developed single nucleotide polymorphism (SNP) markers for a potentially medicinal plant, Crepidiastrum denticulatum (Asteraceae), inferred from complete chloroplast genome data

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