“…Modern methodologies and modifications, such as the development of probes from specificity for highly repeated sequences to single-copy sequence (
Desel et al 2001 ;
Zhu et al 1999 ), and from single-colored probes to multiple-colored probes (
Tang et al 2009 ), have all been designed to optimize the probe detection sensitivity. Nowadays,
FISH is a versatile and accurate tool for chromosome localization of sequences (
Gomez et al 1997 ), cytogenetic map construction (
Sun et al 2013 ;
Han et al 2011 ;
Cui et al 2015 ), genome structure study (
Zhao et al 2011 ; Wang et al 2001a ), genome evolution (
Wu et al 2013 ), and comparative genomics study (
Gan et al 2013 ).…”