Screening acetylcholinesterase inhibitors from marine-derived actinomycetes by simple chromatography

Abstract

Acetylcholinesterase (AchE) is an enzyme involved in the metabolism of Acetylcholine [Ach] cholinergic synapses in the central and peripheral nervous systems. As a result, AChE inhibitors promote an increase in the concentration and length of Ach synaptic activity. Acetylcholinesterase inhibitors (AchEIs) are currently considered the best therapeutic agents for Alzheimer’s patients. The potential sources of the current AChE inhibitors are predominantly plants. However, some of these drugs are known to have disadvantages such as limited half-life or side-effect such as hepatotoxicity. Marine microorganisms are a potential new source of the enzyme inhibitors. Since their rich and diverse metabolites have a wide range of biological activities, one of them is an actinomycetes of special interest in antibiotics. The purpose of this study was to screen AChE inhibitors from marine-derived actinomycetes using a simple method, thin layer chromatography (TLC). In addition, TLC was used for the determination of solvents for adsorption of actinomycetes AchE inhibitors. The results showed that the combination of chloroform/methanol = 80/20 had the best AchE inhibitor separation performance, while the combination of DCM/methanol = 90/10 should be considered if the gradient elution was selected during the separation process.

Investigation of Microplastic Exposure to Marine Fish in the Marine Tourism Area of Makassar City

“…All types of microplastic contaminants identified in the three fish samples obtained from the waters around Samalona Island are secondary microplastics or microplastics originating from human activities. (Kamaruddin, Marzuki, Burhan, & Ahmad, 2021).…”

“…Plankton can eat microplastic, then plankton is eaten by fish, finally, fish is eaten by humans. This process causes microplastics to move from marine biota to humans (Kasamesiri & Taimuangpho, 2020). The dangers of accumulation of microplastics for human health, because they can interfere with health, such as disrupting the nervous, hormonal, and immune systems, and increasing the risk of cancer (Basri et al, 2021).…”

Temporal and Spatial Analysis of Coastal Water Quality to Support Application of Witheleg Shrimp, <em>Litopenaeus vannamei</em> Intensive Pond Technology

“…The complexity of water quality variables in intensive technology whiteleg shrimp ponds is known as an N compound other than nitrate. These compounds arise from the N cycle process, with some of the main ingredients being shrimp feed residues and shrimp faeces [84][85][86]. Tables 5 and 6 show that the ammonia concentration is higher in the dry season when compared to the rainy season in the coastal areas of BB and GT.…”

Comparison of the Strength of Biodegradation of Pyrene between Symbiont Bacteria of Marine Sponges and Bacteria Isolated from Seawater

“…Research on the biodegradation of pollutants in the environment using microorganisms such as bacteria and fungi is still very wide open. It is an interesting research area, including its benefits in the future, to produce new findings in the context of environmental management [80,81]. This research is one of the series and developments of several previous studies carried out on carcinogenic PAHs in the theme of screening for hydrocarbon-degrading bacteria [12,82].…”