Screening and confirmation of microRNA markers for forensic body fluid identification

Wang, Zheng; Zhang, Ji; Luo, Haibo; Ye, Yi; Yan, Jun; Hou, Yiping

doi:10.1016/j.fsigen.2012.07.006

Cited by 116 publications

(76 citation statements)

References 37 publications

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“…The primers (Invitrogen) are shown in Table II and qPCR reaction was performed in the LightCycler ® 480 real-time PCR system (Hoffmann-La Roche, Basel, Switzerland) according to the manufacturer's instructions. The expression levels were calculated using the ΔΔCt method (18).…”

Section: Methodsmentioning

confidence: 99%

miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1

Chen

et al. 2014

International Journal of Molecular Medicine

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Abstract. miR-886-3p has been discovered to be involved in the oncogenesis, progression and metastasis of several types of human cancer. The aim of the present study was to identify the biological function of miR-886-3p in clear cell renal cell carcinoma (ccRCC) and to determine its possible molecular mechanisms. miR-886-3p was found to be significantly upregulated in ccRCC tissues (P<0.05), in accordance with a previous sequencing result. Functional experiments revealed that forced downregulation of miR-886-3p significantly inhibited cellular migration, suppressed cell proliferation and induced cell apoptosis of renal cancer cells. Paired-like homeodomain 1 (PITX1), which has been identified as a tumor suppressor, was found to be downregulated in ccRCC tissues and identified as a target gene of miR-886-3p. Further experiments demonstrated that the protein level, and not the mRNA level, of PITX1 was significantly decreased or increased when miR-886-3p was upregulated or downregulated, respectively, indicating that miR-886-3p acted as an oncogene by directly regulating the protein expression of PITX1 at a post-transcriptional level. In conclusion, this study revealed that miR-886-3p was upregulated in ccRCC and was involved in cellular migration, proliferation and apoptosis of renal cancer cells by directly targeting the tumor suppressor gene, PITX1.

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Section: Methodsmentioning

confidence: 99%

miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1

Chen

et al. 2014

International Journal of Molecular Medicine

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“…Their studies showed a high specificity of miRNA-16 for venous blood, in comparison to other fluids, turning it into an important tool for forensic medicine and criminalistics. 4 Weber et al conducted a similar study, analyzing 12 biological fluids -amniotic fluid, breast milk, bronchial lavage, cerebrospinal fluid, colostrum, peritoneal fluid, saliva, seminal fluid, tears, and urine -in order to obtain a profile of constitutive miRNA expression. 23 They reported hundreds of identified miRNAs for each fluid type, and in order to determine miRNA specificity for each fluid type, they performed further validation tests for these certain species.…”

Section: Specificity Of Mirnas For Biological Fluids and Tissuesmentioning

confidence: 99%

“…This is the reason the analysis of microRNA (miRNA) expression represents a good tool for the future, to be introduced in the forensic laboratory. 1,3,4 miRNAs are short, non-coding RNAs that contain 19-24 nucleotides. From a historical point of view, they were isolated and analyzed for the first time in 1990 in Caenorhabditis elegans.…”

Section: Introductionmentioning

confidence: 99%

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Dumache

Rogobete

Săndesc

et al. 2017

Journal of Interdisciplinary Medicine

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The current practice in the field of forensic medicine imposes the use of modern investigation techniques. The complexity of laboratory investigation methods needed for a final result of the investigation in forensic medicine needed new biomarkers of higher specificity and selectivity. Such biomarkers are the microRNAs (miRNAs), short, non-coding RNAs composed of 19-24 nucleotides. Their characteristics, such as high stability, selectivity, and specificity for biological fluids, differ from tissue to tissue and for certain pathologies, turning them into the ideal candidate for laboratory techniques used in forensic medicine. In this paper, we wish to highlight the biochemical properties and the usefulness of miRNAs in forensic medicine.

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“…Forensic RNA analysis is increasingly being used for more definitive body fluid identification (BFID) with a focus on messenger RNA (mRNA) [1][2][3][4][5] and more increasingly on microRNA (miRNA) [6][7][8][9][10][11]. MicroRNA is, in theory, the preferred marker to target as it is more stable due to its small size (~22-26 bp) and relatively high copy numbers within the cells [11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains

Uchimoto

Beasley

Coult

et al. 2013

Forensic Science International: Genetics

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Screening and confirmation of microRNA markers for forensic body fluid identification

Cited by 116 publications

References 37 publications

miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1

miR-886-3p upregulation in clear cell renal cell carcinoma regulates cell migration, proliferation and apoptosis by targeting PITX1

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains

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