Screening and electrochemical detection of an antibiotic producing gene in bacteria on an integrated microchip

Han, Dawoon; Chand, Rohit; Shin, Ik‐Soo; Kim, Yong−Sang

doi:10.1039/c3ay41321g

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…To achieve the desired temperature for incubation, a calibrated DC power was applied to the ITO layer (Table S1). 33,34 The phosphorylated product was then introduced to the electrochemical cell containing chemosensor modified electrode, for 30 min. Finally the DPV study was carried out in carbonate buffer (0.1 M, 9.2 pH) to measure the change in electrochemical signal due to enzymatic catalysis.…”

Section: Methodsmentioning

confidence: 99%

Gold Nanoparticle Enhanced Electrochemical Assay for Protein Kinase Activity Using a Synthetic Chemosensor on a Microchip

Chand

Han

Shin

et al. 2015

J. Electrochem. Soc.

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Phosphorylation by protein kinases is a predominant form of protein regulation. Abnormal phosphorylation of protein is linked to several medical conditions. In this paper, we report a disposable electrochemical microchip for protein kinase activity assay based on a synthetic chemosensor. The approach involves the phosphate-specific chemosensors linked to gold nanoparticles, anchored on an electrochemical sensor fabricated on the plastic film, and the enzyme substrate labeled with ferrocene tag. In the presence of protein kinase, the substrate undergoes phosphorylation, which subsequently binds to the chemosensor, and then the ferrocene tag of the enzymatic product generates strong oxidation current under voltammetry. Gold nanoparticles were employed as a bridge between the electrode and chemosensor, which significantly enhanced the current signal. A limit of detection of the enzyme was estimated to be 0.05 U/mL with a linear dynamic range between 2-50 U/mL. Furthermore, the interference and inhibition studies were also successfully carried out using this strategy. The method proposes the potential for application in the development of a kinase assay system.

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Section: Methodsmentioning

confidence: 99%

Gold Nanoparticle Enhanced Electrochemical Assay for Protein Kinase Activity Using a Synthetic Chemosensor on a Microchip

Chand

Han

Shin

et al. 2015

J. Electrochem. Soc.

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“…Additionally, most amplified DNA analyses are based on optical methods using colored dye staining [8,[11][12][13]. DNA amplification analysis can be affected by fluorescent dye since it can bind to fragments of template DNA or inhibit enzyme activity [14]. Also, the analysis resolution of optical detection can be severely reduced by turbidity after DNA extraction [3,8,11,15,16].…”

Section: Introductionmentioning

confidence: 99%

PID Temperature Control System-Based Microfluidic PCR Chip for Genetic Analysis

Kim

Schuck

Kim

et al. 2021

J. Electr. Eng. Technol.

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A microfluidic device based on the polymerase chain reaction (PCR) technique for genetic diagnostics was designed and fabricated. The continuous-flow microfluidic PCR system was integrated with two heating blocks to increase the temperature in each zone (denaturation, annealing, and extension). To maintain a constant temperature, heat transfer was controlled by a temperature controller system while receiving real-time feedback of a thermocouple attached to the microchannel. The DNA amplification was evaluated by fluorescence imaging through agarose gel electrophoresis. The developed continuous-flow microfluidic PCR chip provides reliable amplification of the target DNA with a real-time system controller while maintaining the temperature.

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Biophysical and electrochemical approaches for studying molecular recognition of IL-33 binding peptides identified via phage display

Cho

Son²,

Bang

et al. 2022

Analytica Chimica Acta

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Screening and electrochemical detection of an antibiotic producing gene in bacteria on an integrated microchip

Cited by 4 publications

References 31 publications

Gold Nanoparticle Enhanced Electrochemical Assay for Protein Kinase Activity Using a Synthetic Chemosensor on a Microchip

Gold Nanoparticle Enhanced Electrochemical Assay for Protein Kinase Activity Using a Synthetic Chemosensor on a Microchip

PID Temperature Control System-Based Microfluidic PCR Chip for Genetic Analysis

Biophysical and electrochemical approaches for studying molecular recognition of IL-33 binding peptides identified via phage display

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