Screening and Field Trials of Virus Resistant Sources in Capsicum spp.

Abstract

Thirty-seven Capsicum accessions containing cultivated and wild species were screened for resistance to Cucumber mosaic virus (CMV), and were also investigated for their response to Tomato aspermy virus (TAV), Tomato mosaic virus (ToMV), Pepper mild mottle virus (PMMoV), and Tomato spotted wilt virus (TSWV). C. baccatum PI 439381-1-3 (PI 439381-1-3), C. frutescens LS 1839-2-4 (LS 1839-2-4), and C. frutescens cv. Tabasco (cv. Tabasco) showed a hypersensitive reaction against CMV-Y, and thus were not systemically infected. Only inoculated leaves of C. annuum cv. Sapporo-oonaga and cv. Nanbu-oonaga were infected with CMV-Y, and viral infection did not spread systemically. These five accessions (PI 439381-1-3, LS 1839-2-4, cv. Tabasco, cv. Sapporo-oonaga, and cv. Nanbu-oonaga) were considered resistant to CMV-Y. These accessions were also resistant to other CMV isolates, but not to the TAV isolate. PI 439381-1-3, LS1839-2-4, cv. Sapporo-oonaga, and cv. Nanbu-oonaga were susceptible to PMMoV, while PI 439381-1-3 and LS1839-2-4 showed systemic necrosis. All CMV-resistant accessions were susceptible to TSWV. Field tests of eight Capsicum accessions, including CMV, PMMoV, and/or TSWV-resistant accessions, demonstrated that most of the PI 439381-1-3 plants were not infected with CMV and PMMoV among the virus-infested fields. As occurred with mechanical inoculation, LS 1839-2-4, cv. Tabasco, cv. Sapporo-oonaga, and cv. Nanbu-oonaga were hard to infect with CMV in the field.

A Comparative Testing of <i>Cucumber mosaic virus</i> (CMV)-Based Constructs to Generate Virus Resistant Plants

“…Almost all known resistance genes found in several natural sources of Capsicum sp. are partial or polygenic [9][10][11] only few of them confer sustainable resistance [12,13]. Kang et al [14] described a single dominant gene controlling CMV resistance in peppers which was effective against two virus isolates, however, a third one caused infection due to the extreme high variability of CMV.…”

Avaliação da resistência a tobamovirus em acessos de Capsicum spp.

“…O acesso PI 152225, porém, desencadeou reação de hipersensibilidade ao patótipo P 1,2 de PMMoV, e a planta manteve-se assintomática e com ausência de multiplicação viral. Suzuki et al (21) também avaliaram o mesmo acesso de C. chinense quanto à reação a um isolado de PMMoV P 1,2 e concluíram que este possui o gene L 3 . Este acesso pode ser uma fonte potencial para programas de melhoramento, visando à resistência a estes vírus no Brasil, pois atualmente predominam os patótipos P 0 e P 1,2 tanto no campo e em cultivos protegidos, para as quais este gene é efetivo.…”

An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum

“…Several genotypes bearing tolerance/resistance have been reported in C. baccatum, i.e. to pepper fruit anthracnose (AVRDC, 1999;Park, 2005), to cucumber mosaic virus (Suzuki et al, 2003) and particularly in var. Pendulum, to pepper yellow mosaic virus (Bento et al, 2009).…”