Screening and fractionation of plant extracts with antiproliferative activity on human peripheral blood mononuclear cells

Souza-Fagundes, Elaine M.; Queiroz, Ana B. R.; Filho, Olindo Assis Martins; Gazzinelli, Giovanni; Corrêa-Oliveira, Rodrigo; Alves, Tânia Maria de Almeida; Zani, Carlos Leomar

doi:10.1590/s0074-02762002000800024

Cited by 21 publications

(6 citation statements)

References 27 publications

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“…(9) Finally, stem extracts from Sapium glandulatum inhibit the proliferation of phytohaemagglutinin A (PHA)-stimulated peripheral blood mononuclear cells (PBMC). (10) In the present study, we demonstrate that specific subfractions of E. cotinifolia and E. tirucalli greatly induce proliferative or apoptotic activity in PBMC mainly in CD3 + cells. Interestingly, these cells do proliferate in the presence of the E. tirucalli subfraction without the need of accessory cells, suggesting that this subfraction directly activate T-lymphocytes.…”

Section: Research Articlesupporting

confidence: 58%

New promisingEuphorbiaceaeextracts with activity in human lymphocytes from primary cell cultures

Llanes-Coronel

Gámez-Díaz

Suarez-Quintero

et al. 2010

Immunopharmacology and Immunotoxicology

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Section: Research Articlesupporting

confidence: 58%

New promisingEuphorbiaceaeextracts with activity in human lymphocytes from primary cell cultures

Llanes-Coronel

Gámez-Díaz

Suarez-Quintero

et al. 2010

Immunopharmacology and Immunotoxicology

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“…PBMCs were collected from the interphase after Ficoll separation and washed three times in RPMI before further processing. 20 All PBMC cultures were carried out in RPMI medium, supplemented with 5% (v/v) heat-inactivated, pooled AB sera, and 2 mM L-glutamine. An antibiotic/ antimicotic solution containing 1000 U/mL penicillin, 1000 µg/mL streptomycin, and 25 µg/mL fungizone was added to control fungal and bacterial contamination.…”

Section: Cell Culturesmentioning

confidence: 99%

Formation of ion pairing as an alternative to improve encapsulation and anticancer activity of all-trans retinoic acid loaded in solid lipid nanoparticles

Carneiro¹,

Silva²,

Pacheco³

et al. 2012

IJN

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This work aims to develop solid lipid nanoparticles (SLNs) loaded with retinoic acid (RA) to evaluate the influence of two lipophilic amines, stearylamine (SA) and benethamine (BA), and one hydrophilic, triethylamine (TA), on drug-encapsulation efficiency (EE) and cytotoxicity in cancer cell lines. The SLNs were characterized for EE, size, and zeta potential. The mean particle size decreased from 155 ± 1 nm (SLNs without amine) to 104 ± 4, 95 ± 1, and 96 ± 1 nm for SLNs prepared with SA, BA, and TA, respectively. SA-RA-loaded SLNs resulted in positively charged particles, whereas those with TA and BA were negatively charged. The EEs were significantly improved with the addition of the amines, and they increased from 36% ± 6% (without amine) to 97% ± 2%, 90% ± 2%, and 100% ± 1% for SA, TA, and BA, respectively. However, stability studies showed higher EE for BA-RA-loaded SLNs than TA-RA-loaded SLNs after 30 days. The formulations containing SA loaded or unloaded (blank SLNs) with RA were cytotoxic in normal and cancer cell lines. In contrast, the blank SLNs containing TA or BA did not show cytotoxicity in human breast adenocarcinoma cells (MCF-7), while RA-loaded SLNs with the respective amines were significantly more cytotoxic than free RA. Furthermore, the cytotoxicity of BA-RA-loaded SLNs was significantly higher than TA-RA-loaded SLNs. These findings are in agreement with the data obtained in the evaluation of subdiploid DNA content and cell-cycle analysis, which showed better anticancer activity for BA-RA-loaded SLNs than TA-RA-loaded SLNs and free RA. Taken together, these findings suggest that the BA-RA-loaded SLN formulation is a promising alternative for the intravenous administration of RA in the treatment of cancer.

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“…The hexane and methanol fractions were concentrated to dryness using rotary evaporation and kept in sterile containers at 4°C until used. Each fraction was dissolved in (v/v) dimethylsulphoxide (Souza-Fagundes et al 2002).…”

Section: Fractionation Of Crude Plant Extractsmentioning

confidence: 99%

In Vitro and In Vivo Activity of Selected Plant Crude Extracts and Fractions Against Penicillium Italicum

Kanan¹,

Al-Najar²

2009

Journal of Plant Protection Research

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Abstract:The objective of this study is to evaluate (in vitro and in vivo) seven plant extracts and their liquid fractions against four Penicillium italicum isolates. The in vitro study revealed that a concentration 520 µg/ml of crude extract of sticky fleabane leaves or cinnamon bark generated maximum percentage inhibition of 54% and 43%, respectively, against tested fungal isolates. A concentration of 130 µg /ml from each extract (except harmal and garlic where, 390 µg/ml were required) caused complete inhibition of fungal growth of isolates Pi.1 and Pi.3 infecting orange fruit. A concentration of 130 µg/ml of nightshade fruit, fenugreek or sticky fleabane extract inhibited completely the growth of isolates Pi.3 and Pi.5 infecting lemon fruits, whereas a concentration of 390 µg/ml was required to inhibit the growth of isolate Pi.1. Methanolic fractions of cinnamon, garlic or sticky fleabane completely inhibited the growth of fungal isolates. The IC 50 values for these fractions were found to be in the range of: 11.2-24; 30.25-31.50; 25.0-36.0 µg/ml, respectively.A concentration 20 µg/ml of cinnamon hexane fraction inhibited the growth of the fungal isolates, with IC 50 values of 13, 13.75, 14 and 13 µg/ml, respectively, obtained against isolates Pi.1, Pi.3, Pi.5 and Pi.6. The nightshade hexane fraction completely inhibited the growth of isolates Pi.1 and Pi.3 with IC 50 values of 80 and 37.5 µg/ml, respectively. Cinnamon aqueous fraction completely inhibited the growth of isolates Pi.1 and Pi.5 (IC 50 were 61.25 and 58.5 µg/ml, respectively).

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Screening and fractionation of plant extracts with antiproliferative activity on human peripheral blood mononuclear cells

Cited by 21 publications

References 27 publications

New promisingEuphorbiaceaeextracts with activity in human lymphocytes from primary cell cultures

New promisingEuphorbiaceaeextracts with activity in human lymphocytes from primary cell cultures

Formation of ion pairing as an alternative to improve encapsulation and anticancer activity of all-trans retinoic acid loaded in solid lipid nanoparticles

In Vitro and In Vivo Activity of Selected Plant Crude Extracts and Fractions Against Penicillium Italicum

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