Screening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trials

Kimura, Masao; Kobori, Cíntia Nanci; Rodriguez–Amaya, Délia B.; Nestel, Penelope

doi:10.1016/j.foodchem.2005.10.020

Cited by 156 publications

(138 citation statements)

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“…The concentration of total carotenoids was signifi cantly greater in the sticks of the Amarela cassava cultivar (10-12μg g -1 FW) than in the sticks of the 'Cacau' cultivar (5-7μg g -1 FW) at both harvest times, according the F test (Figure 1), as well as observed by KIMURA et al (2007) andCHAMPAGNE et al (2010). This indicates that the 'Amarela' cultivar has almost double the amount of total carotenoids than the white cultivar.…”

Section: Resultsmentioning

confidence: 68%

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Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

et al. 2014

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Section: Resultsmentioning

confidence: 68%

“…KIMURA et al (2007) also observed a decrease of the concentration of carotenoids during storage. The degradation of carotenoids may have occurred due to the oxidative stress suffered by cassava as a function of tissue exposure to oxygen, due to cut into stick form (REILLY et al, 2003;CHAVEZ et al, 2007).…”

Section: Resultsmentioning

confidence: 73%

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

et al. 2014

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“…A linhagem 541332 e a linhagem 541312-1 apresentaram os maiores teores de carotenoides totais, 42,84 e 42,51 µg g -1 , respectivamente, valores esses elevados quando comparados aos valores médios das cultivares brasileiras comerciais, como Assum Preto (11,17 µg g -1 ) e BR 473 (9,17 µg g -1 ), analisadas por Kimura et al (2007), e BRS 1001 (30,95 µg g -1 ), analisada neste trabalho.…”

Section: Resultsunclassified

Variabilidade de genótipos de milho quanto à composição de carotenoides nos grãos

Cardoso

Paes

Galvão

et al. 2009

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi caracterizar e avaliar a variabilidade quanto ao teor e perfi l de carotenoides nos grãos de 134 genótipos de milho (Zea mays), com vistas à utilização em programas de biofortifi cação. Os materiais foram provenientes dos campos experimentais e do Banco Ativo de Germoplasma da Embrapa Milho e Sorgo, Sete Lagoas, MG. São cultivares e híbridos comerciais, linhagens-elite e outros acessos escolhidos com base na coloração amarelo-alaranjada do endosperma. A quantifi cação do teor de carotenoides totais, carotenos e xantofi las mono-hidroxilada e di-hidroxilada dos grãos foi realizada por método cromatográfi coespectrofotométrico. As médias encontradas nos grãos foram 22,34 µg g -1 de carotenoides totais, 2,55 µg g -1 de carotenos, 3,86 µg g -1 de xantofi las mono-hidroxiladas e 15,93 µg g -1 de xantofi las di-hidroxiladas. Os genótipos foram agrupados em 18 grupos pelo método de Tocher. O germoplasma da Embrapa possui potencial para ser usado em programas de desenvolvimento de linhagens de milho biofortifi cadas, quanto ao total de carotenoides pró-vitamina A.Termos para indexação: Zea mays, biofortifi cação, carotenoides, melhoramento, vitamina A. Variability of maize genotypes for grain carotenoid compositionAbstract -The objective of this work was to characterize 134 maize (Zea mays) genotypes, for carotenoids content and build a genetic profi le to facilitate future breeding to increase grain nutritional value (biofortifi cation). Seeds came from experimental fi elds and from the Banco Ativo de Germoplasma of Embrapa Milho e Sorgo, Sete Lagoas, MG, Brazil. The genotypes were commercial hybrids, varieties, and inbred lines developed by the Embrapa biofortifi cation program and other accessions, chosen for their yellow-orange endosperm color. Total grain carotenoids, carotenes, xanthophylls (monohydroxylates and dihydroxylates) were determined by chromatographic-spectrophotometric methods. The detected averages were: 22.34 µg g -1 for total carotenoids, 2.55 µg g -1 for carotenes, 3.86 μg g -1 for monohydroxylated xanthophylls, and 15.93 µg g -1 for dihydroxylated xanthophylls. The genotypes variability was divided into 18 groups by the method of Tocher. The Embrapa germplasm has variability and potential for development of maize lines biofortifi ed for total of provitamin A carotenoids.

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“…HPLC analysis was carried out according to a method validated with a certified reference material [17], adapted to pepper samples in terms of weight of analytical samples, volumes of solvents and reagents, number of times extraction (until the residue was colorless), and chromatrographic conditions. All the necessary precautions were taken to avoid alterations or losses of the carotenoids and other errors during analysis [18].…”

Section: Carotenoid Analysismentioning

confidence: 99%

“…An HPLC method, validated with a certified reference material [17], and a 3 mm C 18 column that allowed baseline separation of the various carotenoids in pepper, were utilized. The different parameters used in identifying carotenoids were also employed.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative differences in the carotenoid composition of yellow and red peppers determined by HPLC‐DAD‐MS

Azevedo-Meleiro

Rodriguez–Amaya

2009

J of Separation Science

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Qualitative and quantitative differences in the carotenoid composition of yellow and red peppers determined by HPLC-DAD-MSThe carotenoids of a yellow (F 1 Amanda hybrid) and a red (F 1 Magali hybrid) pepper (Capsicum annuum L.) at the ripe stage, marketed in Campinas, Brazil, were identified by the combined use of the chromatographic behavior, absorption spectra, and chemical reactions, confirmed by the mass spectra. HPLC was carried out with a Spherisorb ODS 2 (C18, monomeric, 3 mm, 4.6 Â 150 mm) column and DAD and mass detector. The main carotenoids in the yellow pepper were violaxanthin (27.1-36.6 mg/g), lutein (5.3-9.3 mg/g), and b-carotene (1.6-3.9 mg/g). Neoxanthin, (Z)-violaxanthin, (Z)-antheraxanthin, (Z)-lutein, a-cryptoxanthin, b-cryptoxanthin, z-carotene, a-carotene, and phytofluene were also found as minor carotenoids. In red pepper, the major carotenoids were capsanthin (29.2-36.2 mg/g), lutein (5.8-8.7 mg/g), b-carotene (5.1-6.8 mg/g), and violaxanthin (1.8-3.6 mg/g). Minor carotenoids were capsorubin, b-apo-8 0 -carotenal, antheraxanthin, zeaxanthin, and b-cryptoxanthin.

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Screening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trials

Cited by 156 publications

References 4 publications

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

Variabilidade de genótipos de milho quanto à composição de carotenoides nos grãos

Qualitative and quantitative differences in the carotenoid composition of yellow and red peppers determined by HPLC‐DAD‐MS

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