Screening and identification of permeable components in a combined prescription of Danggui Buxue decoction using a liposome equilibrium dialysis system followed by HPLC and LC-MS

Abstract

A new method, i.e., liposome equilibrium dialysis followed by HPLC and LC-MS analysis, has been developed for the screening of permeable components in combined prescriptions of Danggui Buxue decoction (CPDBD). Multiple permeable components were simultaneously predicted by comparison of chromatograms of CPDBD extract before and after interaction with liposome membranes. A diode-array detector (DAD) and an evaporative light scattering detector (ELSD) were used, and the permeable compounds were identified by comparison with the available reference compounds and confirmed by on-line LC-MS. About fifteen compounds in a CPDBD extract were found to interact with liposome membranes. They were identified as calycosin-7-O-beta-D-glucoside (1), senkyunolide I or H (2), ononin (3), (6alphaR,11alphaR)-9,10-dimethoxypterocarpan-3-O-beta-D-glucoside (4), (3R)-2'-hydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside (5), calycosin (6), astragaloside IV (7), isoastragaloside II (8), formononetin (9), (6alphaR, 11alphaR),-3-hydroxy-9,10-dimethoxypterocarpan (10), (3R)-7,2'-dihydroxy-3',4'-dimethoxyisoflavan (11), astragaloside I (12), isoastragaloside I (13), E-ligustilide (14), and Z-ligustilide (15), respectively. Among all permeable components, 1, 3, 6, and 9 (flavonoids), 2, 14, and 15 (phthalides), and 7 (saponins) have been considered as major bioactive components in CPDBD. Therefore, this new method appears useful as a first step in the screening of bioactive components in natural products including Traditional Chinese Medicines (TCMs).

Identification of chemical constituents in SiWu decoction by UHPLC-DAD-TOF/MS

“…For compounds 54, 56, 57, and 61, they were two groups of isomers, by comparison with the reference compounds and referring to the literature [24], they were definitely identified as E-ligustilide, E-butylid-eniphthalide, Z-ligustilide, and Z-butylidenephthalide, respectively.…”

Penetration profile and human cadaver skin distribution of finasteride from vesicular nanocarriers

“…The drug encapsulation efficiency (EE) and loading efficiency (LE) were determined by standard equilibrium dialysis technique (Qi et al, 2006;Rao et al, 2008). Briefly, a known amount of vesicle sample was added into the donor cell.…”

Screening and identification of potential bioactive components in a combined prescription of Danggui Buxue decoction using cell extraction coupled with high performance liquid chromatography

“…astragaloside IV) and phenolics (e.g. ferulic acid) were thought to be the main bioactive compounds in DBD (Ma et al, 2002;Qi et al, 2006;Liu et al, 2002;Chen et al, 2006). Astragaloside IV (13), formononetin (10) and ligustilide (12) were considered as the main bioactive ingredients in DBD obtained by ILC (Sheng et al, 2005;Mao et al, 2002).…”