Screening and induction of laccase activity in fungal species and its application in dye decolorization

Kumar, Vikas; Kirupha, Selvaraj Dinesh; Periyaraman, Premkumar Manickam; Sivanesan, S.

doi:10.5897/ajmr10.894

Cited by 43 publications

(12 citation statements)

References 33 publications

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“…The concentration of glucose used in these studies varied from 0.1 to 1% [38]. The G. lucidum -CDBT1 cultures used herein were optimized to grow in relatively low concentrations (0.4%) of glucose containing media, and under these conditions G. lucidum -CDBT1 culture produced relatively high levels of laccase, while high concentrations of sugars may satisfy the nutrient demands of G. lucidum for growth but do not necessarily promote optimal secretion of laccase.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Physicochemical Characterization of Laccase fromGanoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Shrestha

Joshi

et al. 2016

BioMed Research International

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At present, few organisms are known to and capable of naturally producing laccases and white rot fungi are one such group. In the present study, three fungal species, namely, Ganoderma lucidum-CDBT1, Ganoderma japonicum, and Lentinula edodes, isolated from their native habitat in Nepal were screened for laccase production, and G. lucidum-CDBT1 was found to express highest levels of enzyme (day 10 culture media showed 0.92 IU/mg total protein or 92 IU/mL laccase activity with ABTS as substrate). Lignin extracted from rice straw was used in Olga medium for laccase production and isolation from G. lucidum-CDBT1. Presence of lignin (5 g/L) and copper sulfate (30 μM) in the media increased the extracellular laccase content by 111% and 114%, respectively. The laccase enzyme produced by G. lucidum-CDBT1 was fractionated by ammonium sulfate and purified by DEAE Sepharose anion exchange chromatography. The purified enzyme was found to have a molecular mass of 43 kDa and exhibits optimal activity at pH 5.0 and 30°C. The isolated laccase was thermally stable for up to 70°C for 1 h and exhibited broad pH stability. The kinetic constants, K m, V max, and K cat, determined using 2,2′-azinobis-(-3-ethylbenzothiazoline-6-sulfonic acid) as substrate were found to be 110 μM, 36 μmol/min/mg, and 246 min−1, respectively. The isolated thermostable laccase will be used in future experiments for delignification process.

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Section: Discussionmentioning

confidence: 99%

Isolation and Physicochemical Characterization of Laccase fromGanoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Shrestha

Joshi

et al. 2016

BioMed Research International

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“…The laccase activity was assayed using 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as substrate (Rasera et al, 2009). The laccase reaction mixture contained 0.5 ml of 0.45 mM ABTS, 1.2 ml of 0.1 M phosphate buffer (pH 6.0) and 0.5 ml of enzyme sample (Vaidyanathan et al, 2011). The oxidation of the substrate (ABTS) was read at absorbance of 420 nm, molar extinction co-efficient of 3.6 × 10 4 cm -1 M -1 .…”

Section: Morphological and Molecular Characterization Of White Rot Fungimentioning

confidence: 99%

“…The liquid broth was prepared (Sayadi and Ellouz, 1995;Vaidyanathan et al, 2011) and the white rot fungi, P. pulmonarius, P. sajar-caju, S. commune were inoculated. The secretion of lignolytic enzymes were assessed at different pH (3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) and at different temperatures (20, 25, 30, 35, 40, 45, 50 and 55°C) and different time interval ( 1 st day up to 15 th day).…”

Section: Production Of Laccase Lip and Mnp By Selected White Rot Funmentioning

confidence: 99%

“…Previous studies include screening with various kinds of azo, anthraquinone, heterocyclic, triphenyl methane and polymeric dyes to detect the enzyme secretion by P. chrysosporium (Paszezynski et al, 1991). Though laccase can oxidise a variety of substrates like phenolic compounds and aromatic amines, indicators like guaiacol are widely used as substrates for laccase production (Coll et al, 1993;Vaidyanathan et al, 2011). Also inducers like ABTS have been widely used which forms green color in the growth medium due to the oxidation of ABTS 2,22 -Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) to ABTS-azine in the presence of laccase.…”

Section: Screening Of White Rot Fungi For Lignolytic Enzymesmentioning

confidence: 99%

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Extracellular secretion of lignocellulolytic enzymes by diversewhite rot asidiomycetes fungi

Thiribhuvanamala¹,

Kalaiselvi²,

Parthasarathy³

et al. 2017

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Rich source of biota includes several white rot fungi, were reported in Western Ghats regions of Tamil Nadu, India. The effective identification of white rot fungi and their lignocellulolytic enzymes play a crucial role for degradation of agricultural and industrial wastes to maintain su sta ina ble ecosystem. T hus, the present study was conducted to assess the secretion of lignocellulolytic enzymes of diverse white rot fungi, collected from Western Ghats regions of Tamil Nadu, comprising of Kotagiri and Ooty of the Nilgiri district, Siruvani and Anaikati of Coimbatore district. A total of 16 white rot fungi belonging to 6 genera, viz., Pleurotus spp., Lentinus spp., Ganoderma spp., Trametes spp., Pycnoporus spp. and Schizophyllum spp. were collected and identified through morphological and molecular methods. The activity of cellulase enzyme was prominently visible in Pleurotus pulmonarius, P. sajar-caju, Pycnoporus sanguineus, P. ostreatus, Ganoderma gibbosum and Schizophyllum commune and hemicellulolytic activity was maximum in Trametes ijubarskii, Lentinus crinitus, P. sanguineus and G. gibbosum . The quantitative and qualitative screening for major lignolytic enzymes, viz., laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP) revealed significant levels of Lac secretion when compared to LiP and MnP by P. pulmonarius, P. sajar-caju and Schizophyllum commune. The syringaldazine well test further confirmed the secretion of laccase as major enzyme followed by LiP and MnP. Also the Lac and LiP activity was maximum at pH of 4.5 and MnP activity at pH of 5.0 by P. pulmonarius, P. sajar caju and S. commune. Temperature of 30°C induced maximum secretion of Lac, LiP and MnP by these three fungi. A significant level of Lac was observed on 4 th day after inoculation while LiP and MnP was maximum on 7 th and 8 th day for P. pulmonarius and P. sajar-caju. Whereas, S. commune secreted significant levels of Lac on 10 th to 11 th da y, while LiP and MnP on 6 th and 7 th da y. This study paves the way for u se of P. pulmonarius, P. sajar-caju and S. commune for biodegradation and bioconversion of agrowastes.

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“…Some of these applications are already in practice, such as pulp processing (Virk et al 2012), detoxification of environmental pollutants (Harms et al 2011), preventing wine browning (Osma et al 2010), oxidation of dyes and their precursors (Kumar et al 2011), and producing lignin from cellulosic material (Wang et al 2014). Laccase has been used commercially as a potential delignification agent in pulp production since the last decade (Camarero et al 2007;Rico et al 2014;Wang et al 2014).…”

Section: Laccase Application In Fiberboard Fabricationmentioning

confidence: 99%

Laccase, an Emerging Tool to Fabricate Green Composites: A Review

Mahmood¹,

Hashim²,

Sulaiman³

et al. 2015

BioResources

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In the last two decades, laccases have received much attention from researchers because of their specific ability to oxidize lignin. This function of laccase is very useful for applications in several biotechnological processes, including delignification in the pulp and paper industry and the detoxification of industrial effluents from the textile and petrochemical industries. This review focuses on laccase-mediated fiberboard synthesis. Growing concerns regarding the emission of formaldehyde from wood composites has prompted industrialists to consider the fabrication of green composites. Laccase-mediated fiber treatments oxidize the lignin component without affecting the cellulose structure. As a result, free radicals are generated on the fiber surface, and these can act as potential reactive sites for further cross-linking reactions in board manufacturing. Binderless fiberboards prepared using such methods can be considered as green composites because the manufacturing process involves no additional resin.

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Screening and induction of laccase activity in fungal species and its application in dye decolorization

Cited by 43 publications

References 33 publications

Isolation and Physicochemical Characterization of Laccase fromGanoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Isolation and Physicochemical Characterization of Laccase fromGanoderma lucidum-CDBT1 Isolated from Its Native Habitat in Nepal

Extracellular secretion of lignocellulolytic enzymes by diversewhite rot asidiomycetes fungi

Laccase, an Emerging Tool to Fabricate Green Composites: A Review

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