Screening and Survival Analysis of Hub Genes in Gastric Cancer Based on Bioinformatics

Zheng, Shunxin; Yang, Lu; Dai, Ying; Jiang, Lifang; Wei, Yi; Wen, Hongwei; Xu, Yingfang

doi:10.1089/cmb.2019.0119

Cited by 17 publications

(14 citation statements)

References 24 publications

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“…In addition, knockdown of CTSL could sensitize cancer cells to chemotherapy and target therapy [19,20]. Our study found that CTSL was up-regulated in gastric cancer compared to normal tissues, which is consistent with the ndings in previous studies [21]. Moreover, CTSL overexpression was found to negatively correlate with prognosis of gastric cancer in this study, which might be explained by that CTSL could induce the migration, invasion and epithelial-mesenchymal transition of cancer cells [18,22].…”

Section: Discussionsupporting

confidence: 89%

Identification Of The Molecular Targets And Immunophenotype Of Gastric Cancer By Bioinformatics Analysis

Yang¹,

Zhang²,

You³

et al. 2020

Preprint

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Background: Gastric cancer (GC) is the most lethal tumor of gastrointestinal tract worldwide. Despite advances in various therapies, the prognosis of GC remains poor. Moreover, only a small fraction of GC patients benefit from immunotherapy. Therefore, it is urgent to deeply understand the molecular characteristics and immunophenotype of GC. Methods: We analyzed the gene expression profile of GSE118916 from GEO database, including the mRNA expression profiles of 15 pairs of GC tumor and adjacent non-tumor tissues. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the online website DAVID. And then the survival prediction values of the top 10 up-regulated genes were analyzed using Kaplan–Meier plotter database. Finally, the immune cells infiltration was analyzed using CIBERSORT online tool.Results: A total of 1156 DEGs were identified, including 633 up-regulated genes and 523 down-regulated genes. The up-regulated genes were mainly enriched in cell adhesion, proliferation, migration and inflammation response. In addition, the up-regulated genes were significantly enriched in acid metabolism, complement and coagulation cascades, cell adhesion and p53 signaling pathway, which were all significant in tumor progression, relapse and metastasis. In addition, the up-regulated genes CTSL and PIEZO1 were associated with poor prognosis in GC patients. Moreover, a unique immune-suppressive microenvironment was identified in GC tissues. Conclusions: CTSL and PIEZO1 might be potential biomarkers and therapeutic targets in GC patients.

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Section: Discussionsupporting

confidence: 89%

Identification Of The Molecular Targets And Immunophenotype Of Gastric Cancer By Bioinformatics Analysis

Yang¹,

Zhang²,

You³

et al. 2020

Preprint

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“…SERPINH1 protein expression was significantly higher in colorectal cancer patients with lymph node metastasis [15]. Zheng et al analyzed the GEO database and showed that SERPINH1 was one of the five hub genes in GC [28]. Cao et al analyzed plasma mRNA profiles of 56 GC patients and 14 healthy individuals in the Oncomine database and demonstrated high SERPINH1 mRNA levels in the GC samples [29].…”

Section: Discussionmentioning

confidence: 99%

SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/β-catenin signaling pathway

Tian

Peng

et al. 2020

Aging

143

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In this study, we investigated the role of SERPINH1 in gastric cancer (GC) progression. GC patient tissues show significantly higher SERPINH1 mRNA and protein levels than normal gastric mucosal tissues. GC patients with high SERPINH1 expression are associated with lymph node metastasis and poor prognosis. SERPINH1 mRNA levels negatively correlate with E-cadherin mRNA levels and positively correlate with levels of N-cadherin, MMP2, and MMP9 mRNA levels. This suggests SERPINH1 regulates epithelial to mesenchymal transition (EMT). SERPINH1 expression was significantly higher in the HGC-27, AGS, MGC-803, and SGC-7901 GC cell lines than in the GES-1 normal gastric mucosal cell line. In SERPINH1-silenced SGC-7901 cells, survival, colony formation, migration and invasion were all reduced, whereas they were all enhanced in SERPINH1-overexpressing MGC-803 cells. Levels of WNT/β-catenin signaling pathway proteins, including β-catenin, Wnt2, GSK-3β, p-GSK-3β, NF-κB P65, Snail1, Slug and TWIST, were all reduced in SERPINH1-silenced SGC-7901 cells, and increased in the SERPINH1-overexpressing MGC-803 cells. Inhibition of SERPINH1 protein using Co1003 significantly decreased survival, invasion, and migration of GC cells. SERPINH1 thus appears to regulate EMT and GC progression via the Wnt/β-catenin pathway, making SERPINH1 a potential prognostic biomarker and therapeutic target in GC patients.

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“… 77 However, by contrast, a bioinformatics screening for hub genes associated with GC by Zheng et al indicated that GPER was highly expressed in GC tissues and that the overexpression of GPER was associated with poor survival. 78 …”

Section: Gper In Gastric Cancer (Gc)mentioning

confidence: 99%

“…However, a study by Zheng et al provided inconsistent evidence. 78 The possible reason is that this study only carried out clip data and a bioinformatics analysis and lacked clinical data and in vitro or in vivo experimental validation. Furthermore, the database and research tools used in this study were different from those used in a previous study.…”

Section: Gper In Gastric Cancer (Gc)mentioning

confidence: 99%

Role of G Protein-Coupled Estrogen Receptor in Digestive System Carcinomas: A Minireview

Qiu¹,

Xiong²,

Yu³

2021

OTT

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Digestive system carcinomas are one of the leading causes of cancer-related deaths worldwide. G protein-coupled estrogen receptor (GPER), a novel estrogen receptor, has been recognized as an important mediator in numerous cancer types. Recently, the function and clinical significance of GPER in digestive system carcinomas has been a subject of interest. Increasing evidence has revealed that GPER plays an important role as a potential biomarker in digestive system carcinomas. This work summarizes the recent literature and focuses on the emerging functional role of GPER in digestive system carcinomas, including gastric cancer, hepatocellular carcinoma, pancreatic cancer, and colorectal cancer. The potential application of GPER in novel strategies for the diagnosis and treatment of digestive system carcinomas is discussed and highlighted.

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Screening and Survival Analysis of Hub Genes in Gastric Cancer Based on Bioinformatics

Cited by 17 publications

References 24 publications

Identification Of The Molecular Targets And Immunophenotype Of Gastric Cancer By Bioinformatics Analysis

Identification Of The Molecular Targets And Immunophenotype Of Gastric Cancer By Bioinformatics Analysis

SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/β-catenin signaling pathway

Role of G Protein-Coupled Estrogen Receptor in Digestive System Carcinomas: A Minireview

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