Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEX

Fang, Yu; Chen, Jing; Wang, Zecheng; Yang, Hongwei; Li, Hui; Jia, Wenchao; Xue, Shuyuan; Xie, Hexin; Xu, Danke

doi:10.1016/j.talanta.2020.121750

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…Molecular docking simulations are usually used to investigate the interactions between aptamers and their targets [ 10 ]. However, in this work, we used the simulation to investigate the effect of CDNA region on the recognition between DSA1N5C and S1 protein.…”

Section: Resultsmentioning

confidence: 99%

“…The issue is to explore the specific molecular recognition element with sensitive signal amplification system. Aptamers, known as ‘chemical antibodies’, are short sequences of synthetic oligonucleotides with comparable affinity to antibodies [ 10 ]. Due to their small size, flexible structure, low cross reactivity and immunogenicity, aptamers are highly suited as the recognition elements in SARS-CoV-2 sensors [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplification

Wang

Zhang

et al. 2023

Talanta

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplification

Wang

Zhang

et al. 2023

Talanta

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“…However, Cell-SELEX procedures allow selecting aptamers that interact directly with cell surface components in their native conformation with no prior knowledge of the molecular target. Since the complex components on the cell surface may bias the library towards highly expressed epitopes, several combinatorial strategies have been reported such as precision-SELEX [ 30 ] or cross-over SELEX [ 31 ]. Recently, both SELEX and Cell-SELEX have been implemented to generate the possible aptamers for different pathogens and their subspecies, such as Escherichia coli [ 32 ], Staphylococcus aureus [ 33 ], Bacillus cereus [ 34 ], Streptococcus agalactiae [ 35 ], Rikinella microfusus [ 36 ], and many other medical and food-born pathogenic bacteria [ 37 , 38 ], to utilize them in biosensing or purification applications.…”

Section: Introductionmentioning

confidence: 99%

Aptamer decorated PDA@magnetic silica microparticles for bacteria purification

Kavruk,

Babaie,

Kibar

et al. 2024

Microchim Acta

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One significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering factors such as blood in the sample matrix. In the present investigation, a specific aptamer binding was chosen for its affinity, while exhibiting no binding affinity towards non-target bacterial cells. This selective binding property was leveraged to facilitate the production of magnetic microparticles decorated with aptamers. A novel assay was developed to effectively isolate S. pneumoniae from PBS or directly from blood samples using an aptamer with an affinity constant of 72.8 nM. The capture experiments demonstrated efficiencies up to 87% and 66% are achievable for isolating spiked S. pneumoniae in 1 mL PBS and blood samples, respectively. Graphical abstract

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“…They developed a BSA beads coated anti-P. aeruginosa aptamers, which successfully captured cells of this pathogen in human fluids.So far, none of the studies regarding aptamers for Gram-negative bacteria have approached specifically antibiotic resistant bacteria. Chen et al, 2020 and Yu et al, 2021[50,51], selected aptamers specifically for KPC-2 producing bacteria (XK10) in a method called Precision-SELEX. In this method, they combined protein SELEX and bacterium SELEX running first four rounds of protein SELEX using KPC-2 as target and subsequently four rounds of conventional bacteria SELEX using E. coli KPC-2 producer.…”

mentioning

confidence: 99%

Application of aptamer technology in enterobacteria and non-fermenters: literature review

Bes

Farrel

Santos³

et al. 2022

Preprint

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Antimicrobial resistance has grown exponentially in the last decade and become a global health threat. The antibiotic resistance crisis has guided the scientific community to explore non-conventional interventions to target resistant bacteria. Development of new technologies, such as aptamers-based treatment and diagnosis, has shown to be promising with remarkable advantages over the past five years. This narrative review aims on what is already known regarding application of aptamer technology in enterobacteria and non-fermenters, and the prospects for future achievements. A systematic search of the English literature was performed on the 7th of December 2021 to identify papers on aptamer discovery, with a focus on gram negative isolates, published from January 01, 1993, to December 07, 2021, under the topics: (aptamer OR aptamers OR SELEX) AND (bacteria OR sepsis OR non-fermenter OR Enterobacteriaceae OR infection)). The reference lists of included articles were also searched, in addition to hand-searching of various relevant high-impact journals. Out of 2,474 articles, 30 experimental studies were recruited for review, and are chronologically described. Although the number of publications regarding development of aptamers to target these pathogenic agents has increased over the years, the recent publications are mostly around diagnostic devices manufactured using previously described aptamers. There have been less than one-third of the studies describing new and specific aptamers. From the 30 selected papers, 18 are regarding non-fermenters, seven approaching multi-species of bacteria and only five regarding a single enterobacteria. Even for the newly described aptamers, most of the published papers pertain to diagnostic aptamers and only seven focus on aptamers for therapeutics. The number of aptamers with strong and specific binding capacity are still limited. Improving the current SELEX and developing more APT remains the major hurdle for aptamer related studies.

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Screening aptamers for serine β-lactamase-expressing bacteria with Precision-SELEX

Cited by 7 publications

References 36 publications

An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplification

An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplification

Aptamer decorated PDA@magnetic silica microparticles for bacteria purification

Application of aptamer technology in enterobacteria and non-fermenters: literature review

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