Screening for and Characteristics of Microbial Flocculants

Kurane, Ryuichiro; Takeda, Kiyoshi; Suzuki, Tomoo

doi:10.1080/00021369.1986.10867746

Cited by 45 publications

(65 citation statements)

References 6 publications

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“…The cell morphology and gram staining of the strain was examined after being cultured for 20 h by microscopy (magnification; ×1000). The utilization of some carbon sources by the strain was performed based on Bergey's Manual of Systematic Bacteriology (Kurane et al 1986). …”

Section: Isolation and Identification Of Xj-25mentioning

confidence: 99%

Antioxidant activities of Bacillus simplex XJ-25 isolated from sand biological soil crusts and its properties

Wang¹,

Sheng²,

Tian³

et al. 2013

Afr. J. Microbiol. Res.

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XJ-25, a strain with strong antioxidant activity was isolated from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China. Strain XJ-25 is closely related to Bacillus simplex through the 16s rDNA sequencing combined with morphological, physiological and biochemical analysis. The medium for optimal antioxidant activity was NB with 1.5 g/L glucose. Based on kinetic assay, antioxidant activity began at early exponential growth phases; maximum activity was reached at the stationary phase. Scavenging effects on DPPH, the hydroxyl and superoxide radicals, Total antioxidant capacity (T-AOC) and protection against lipid damage were evaluated. The main antioxidant compounds were in the extracellular secreted supernatant of XJ-25. The active compounds were very stable at the pH range of 2 to 12, temperature from 40°C to 121°C, as well as in some organic solvents. Thin layer chromatography assay by DPPH scavenging assay show two active spots with Rf values of 0.35 and 0.47 and both of them were ninhydrin positive.

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Section: Isolation and Identification Of Xj-25mentioning

confidence: 99%

Antioxidant activities of Bacillus simplex XJ-25 isolated from sand biological soil crusts and its properties

Wang¹,

Sheng²,

Tian³

et al. 2013

Afr. J. Microbiol. Res.

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“…Flocculation activities were calculated and measured using a modified Kaolin clay suspension method (Kurane et al, 1986). A mixture of 5 g/L synthetic clay suspension (Sigma-Aldrich, 0.1-4 µm particle size) with 0.5 mL bioflocculant solution (culture broth, cell-free supernatant or extracted biopolymer) in the presence of CaCl 2 (as coagulant) was stirred with rapid mixing at 230 rpm for 2 min, followed by slow mixing at 80 rpm for 3 min using Laboratory Flocculator (Jar Tester Model CZ150) and left standing for 5 min.…”

Section: Determination Of Flocculation Activitymentioning

confidence: 99%

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Zaki

Farag

Elreesh

et al. 2011

Int. J. Environ. Sci. Technol.

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ABSTRACT:The aim of this study was to look for high efficient bioflocculant-producing microorganisms. Among 36 bacterial colonies isolated from a crude petroleum oil sample, three of them as Bacillus subtilis and Pseudomonas spp. exhibited flocculation activity exceeding 90 % after 3 days of cultivation. They were identified by 16 S rDNA sequence analysis as Bacillus subtilis and Pseudomonas sp. Spectroscopic analysis of the polymers by nuclear magnetic resonance and fourier-transform infrared revealed that the polymers were glycoproteins. These polymers were soluble in water and insoluble in any organic solvents tested. The effects of bioflocculant dosage, temperature and pH on the flocculation activity were evaluated. The maximum bioflocculation activities were observed at an optimum bioflocculant dosage of 3.5 mg/L (strains Bacillus subtilis and Pseudomonas) and 5.0 mg/L (strain CPO14), respectively. In addition, these biopolymers were able to flocculate kaolin suspension (5 g/L) over a wide range of pH (pH 3-9) and temperature (5-50 °C) tested in the presence of CaCl 2 . The highest flocculation activities of strains CPO8, CPO13 and CPO14 were 96.03 %, 92.17 % and 97.59 %, respectively in the early stationary phase (at 24 h), while the cell production reached its maximum in the stationary phase (at 72 h). Their efficient flocculation capabilities suggest potential applications in industries.

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“…The method by Kurane et al (1986) was adapted to access the flocculating activity. 100 ml of Kaolin clay solution (4 g/1000 ml) was mixed with 3 ml of 1% w/v CaCl2 and 2 ml of obtained supernatant.…”

Section: Determination Of Flocculating Activitymentioning

confidence: 99%

Production and characteristics of bioflocculant TPT-1 from a consortium of Bacillus pumilus JX860616 and Alcaligenes faecalis HCB2

Maliehe¹,

Selepe²,

Ntombela³

et al. 2016

Afr. J. Microbiol. Res.

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Screening for and Characteristics of Microbial Flocculants

Cited by 45 publications

References 6 publications

Antioxidant activities of Bacillus simplex XJ-25 isolated from sand biological soil crusts and its properties

Antioxidant activities of Bacillus simplex XJ-25 isolated from sand biological soil crusts and its properties

Characterization of bioflocculants produced by bacteria isolated from crude petroleum oil

Production and characteristics of bioflocculant TPT-1 from a consortium of Bacillus pumilus JX860616 and Alcaligenes faecalis HCB2

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