Screening for cytotoxic activity of extracts and isolated alkaloids from bulbs of Hippeastrum vittatum

Silva, A.F.S.; Andrade, Jean Paulo de; Machado, Katia R.; Rocha, Adriana Brondani da; Apel, Miriam Anders; Sobral, Marcos; Henriques, Amélia T.; Zuanazzi, José Ângelo Silveira

doi:10.1016/j.phymed.2007.12.001

Cited by 46 publications

(43 citation statements)

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“…The dichloromethane extracts, three alkaloids (lycorine, vittatine and montanine) exhibited potential antiproliferation activity in MCF7 breast cancer cells (Silva et al, 2008). Furthermore, it could induce the apoptosis of MCF7 (Ji et al, 2017).…”

Section: Discussionmentioning

confidence: 97%

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Chen

2018

Bangladesh J Pharmacol

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Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling BJP IntroductionBreast cancer is the second leading cause of cancer death among women after lung cancer in the United States (DeSantis et al., 2014). In the treatment for breast cancer, some adverse effects could cause serious complications and affect the health and quality of life among survivors (Odle, 2014). Therefore, it is necessary to develop novel anti-cancer candidates for breast cancer.In the past several decades, natural compounds such as resveratrol (Sinha et al., 2016), epigallocatechin-3-gallate, oldenlandia diffusa and Ziziphus jujube have been demonstrated that possessed potential anti-breast cancer activity (Bonofiglio et al., 2016).Lycorine, known as a multifunctional compound, is extensively studied for its pharmacological activities, such as anti-tumor activity (Lamoral-Theys et al., 2010). Previous studies have shown that lycorine presented a potential anti-cancer activity in breast cancer via the inhibition of tumor growth and metastasis Ying et al., 2017). Src/FAK (focal adhesion kinase) -involved pathway and STAT3 (signal transducer and activator of transcription 3) signaling pathway were involved in growth, invasion and metastasis of breast cancer.However, Wnt/ -catenin signaling, a multifunctional pathway that has not been studied to be involved in proliferation, invasion and migration of breast cancer. This study is meant to confirm the inhibitory effects of lycorine on the proliferation, migration and invasion of MDA-MB-231 breast cancer cells and investigate the activation of Wnt/ -catenin signaling pathway involved. Materials and Methods Cell lines and reagentsHuman MDA-MB-231 breast cancer cells were obtained AbstractThis study was aimed to determine the effects of lycorine, a toxic crystalline alkaloid, on MDA-MB-231 breast cancer cells proliferation, migration and invasion, and to investigate the mechanism involved. The cells were cultured with different concentrations of lycorine in vitro. MTT assays were performed to determine the proliferation of cells. Transwell assays were performed to measure the migration and invasion of cells. The activation of Wnt/ -catenin signaling pathway and expression were assayed by Western blot. This study showed that proliferation, migration and invasion of MDA-MB-231 breast cancer cells could be inhibited by lycorine. Furthermore, we found that Wnt/ -catenin signaling was markedly blocked in MDA-MB-321 cells treated with lycorine. In conclusion, lycorine inhibits the proliferation, migration and invasion of MDA-MB-231 breast cancer cells that is associated with the suppression of Wnt/ -catenin signaling. Article Info

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Section: Discussionmentioning

confidence: 97%

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Chen

2018

Bangladesh J Pharmacol

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“…This compound also possesses antimalarial activity against strains of chloroquine-sensitive Plasmodium falciparum, hypotensive effects and antiretroviral activity (Bastida et al, 2006;Kaya et al, 2011). Vittatine (24) and maritidine (30) have shown cytotoxic activity against HT29 colon adenocarcinoma, lung carcinoma and RXF393 renal cell carcinoma (Bastida et al, 2006;Silva et al, 2008). Antibacterial activity against Gram-positive Staphylococcus aureus and Gram-negative E. coli have been reported for vittatine (24) and 11-hydroxyvittatine (28) (Kornienko and Evidente, 2008).…”

Section: Haemanthamine Typementioning

confidence: 99%

The Genus Galanthus: A Source of Bioactive Compounds

Berkov¹,

Codina²,

Bastida³

2012

Phytochemicals - A Global Perspective of Their Role in Nutrition and Health

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“…They have been of specific interest due to their physiological activities including anticancer, antiviral and antimalarial activities 1 . There are a number of antitumor agents having topo-isomerases as their target and they act through different mechanisms such as preventing DNA-topoisomerase binding, inhibiting the cleavage of DNA or stabilizing the topoisomerase-DNA cleavable complex 2 -4 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2017

IJPSR

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ABSTRACT:To investigate the content of polar auxin transport inhibitor flavonoid quercetin (Q) and topo-isomerase I-DNA inhibitor alkaloid camptothecin (CPT) from Gracilaria corticata (GC), red seaweed from Surathkal beach, Karnataka a comparative study was carried out. Methods: The content of Q and CPT was quantified in the ethanolic extract of GC using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The HPLC analysis resulted in a sharp peak of authentic CPT and Q at the retention time of 2.445 min and 3.540 min respectively. The peaks of ethanolic extract of GC spiked with CPT was found at the retention time of 1.630 min, 1.690 min and 2.000 min and retention time in the ethanolic extract of GC spiked with Q was found to be 1.600 min and 1.713 min. The method developed for this study shall stand appropriate for the quantification of secondary metabolites and screening of flavonoid and alkaloid contents in the seaweeds.

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Screening for cytotoxic activity of extracts and isolated alkaloids from bulbs of Hippeastrum vittatum

Cited by 46 publications

References 12 publications

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

Lycorine inhibits MDA-MB-231 breast cancer cell proliferation, migration and invasion that are associated with Wnt/β-catenin signaling

The Genus Galanthus: A Source of Bioactive Compounds

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