Screening for marine nanoplanktic microalgae from Greek coastal lagoons (Ionian Sea) for use in mariculture

Abstract

Mediterranean mariculture uses imported strains of marine phytoplankton, raising questions of ecological risk and ability to adapt to local conditions for mass culture outdoors. In this context, we report here on the mass-culture potential and chemical composition of six strains of Prasinophyceae (five strains of Tetraselmis sp. and one Pyramimonas sp.) isolated from a Greek coastal lagoon. Proximate composition had a pattern of 10-20% ash, 35-65% protein, 6-10% lipids, and 25-45% other organics including carbohydrates. The amino acid profiles were typical for the marine representatives of the class. All strains had a high PUFA content with dominant the ω3 fraction in four of them. The fatty acid profiles indicated a Tetraselmis strain with high EPA (14%) and a Pyramimonas strain with high DHA (6%). These strains might be a good alternative for the common commercial strains used in Mediterranean aquaculture.

Total fish meal replacement with canola protein isolate in diets fed to rainbow trout (Oncorhynchus mykissW.)

“…Raw material and dietary amino acid concentrations were analysed as described by Tzovenis et al . ().…”

Total lipids content and fatty acids composition of the rotifer Brachionus plicatilis using artificial enrichments

“…The nutritional value of rotifers as live feed for fish larvae was studied intensively and continuously improved, as dietary value impacted larval growth and development (Oka et al, 1980;Rønnestad et al, 2013). Several rearing trials of rotifers fed with algal species and strains in various forms (fresh, frozen, dried, frozen-dried, concentrated, frozen concentrated, etc) have been carried out in order to support the population growth and survival of the rotifer cultures as well as improve the dietary value of Brachionus plicatilis (Scott and Baynes, 1978;Yufera and Pasqual, 1983;Yamasaki et al, 1984;Lubzens et al,1995;Yufera and Navarro, 1995;Navarro and Sarasquete, 1998;Robert and Trintignac, 1997;Maruyama et al, 1997;Yoshimura et al, 1997;Tzovenis, et al, 2004;Palmtag et al, 2006;Tzovenis et al, 2009;Seychelles et al, 2009;Kotani et al, 2010). In addition, a range of artificial enrichments such as yeasts (Kitajima et al,1979;Hirata, 1980, Kitajima et al, 1980aPenglase et al,2011), microcapsules (Jones et al, 1984;Walford and Lam, 1987;Langdon, 2003) and emulsions (Dhert et al, 1993;Haché and Plante, 2011) were continuously tested in an effort to improve and optimize the nutritional value (total lipids, fatty acids, trace elements, vitamins, amino acids) of the rotifers as they used as biocapsule-boosters to transfer nutrients and support fish larval growth (Lubzens et al, 1989;Coutteau and Sorgeloos, 1997;Rodríguez et al, 1996;Dhert et al, 2001;Lubzens and Zmora, 2003;Conceição et al, 2010).…”

Microalgae metabolites: A rich source for food and medicine

“…, Pyramimonas spp . Infant formula; Nutritional supplements; Aquaculture feedChu, 2012, Tzovenis et al, 2009Eicosapentaenoic acid (EPA) Pavlova spp. , Nannochloropsis spp.…”

“…, Monodus spp., Phaeodactylum spp. , C. minutissima, C. vulgaris, Nannochloris spp ., N. atomus, Tetraselmis spp ., T. suecica Nutritional supplements; Aquaculture feedChu, 2012, Seto et al, 1984, Ben-Amotz et al, 1985, Reitan et al, 1994, Patil et al, 2007, Tzovenis et al, 2009, D’Souza and Loneragan, 1999γ-linolenic acid (GLA) Spirulina spp ., C. homosphaera, Chlorococcum spp., D. primolecta Nutritional supplementsChu, 2012, Chiang et al, 2004, Ohta et al, 1995Hexadecatetraenoic acid Ankitrodesmus spp ., Chlamydomonas spp ., C. moewusii, D. bardawil, D. tertiolecta, T. suecica, T. cylindrica -Ben-Amotz et al, 1985, Arisz et al, 2000, Fried et al, 1982, D’Souza and Loneragan, 1999, Gouveia and Oliveira, 2009, Becker, 2004Linolenic acid B. braunii, C. moewusii, C. protothecoides, C. vulgaris, Chlorococcum spp ., D. bardawil, D. primolecta, D. tertiolecta, N. atomus, N. oleaobundans, P. subcapitata, S. obliquus, T. suecica Anti-inflammatory; Acne reductive; Moisture retentive propertiesChiang et al, 2004, Arisz et al, 2000, Day et al, 2009, Piorreck et al, 1984, Fried et al, 1982, Gouveia and Oliveira, 2009, Reitan et al, 1994, Patil et al, 2007, D’Souza and Loneragan, 1999Stearidonic acid M. pusilla, T. suecica Increasing tissue EPA concentrations in tissuesFernandez et al (2006); D’Souza and Loneragan (1999) 9. Proteins Glycoprotein C. vulgaris, Anticancer; Anti-inflammatory activity; Anti-photoagingHasegawa et al, 2002, Tanaka et al, 1998Phycocyanin S. platensis Natural dye for health food and cosmetics (lipsticks and eyeliners) antioxidantChu (2012)Phycoerythrin P. cruentum Fluorescent agent; Tool for biomedical research; DiagnosticChu (2012) 10.…”