Screening for potential quality markers of Callerya nitida var. hirsutissima. Z.Wei based on components profile, pharmacokinetics, and anti‐inflammatory study

Abstract

Callerya nitida var. hirsutissima. Z.Wei is a classical, traditional Chinese herbal medicine mostly used to treat rheumatoid arthritis. Recent reports suggest that inconsistent and poor-quality control levels have primarily affected the therapeutic efficacy. Therefore, the aim of the current study was to investigate the active chemical ingredients, stability of components in blood, pharmacokinetics, and pharmacodynamics to specify the potential markers for quality control and quality evaluation of Callerya nitida. The active components in vitro and in vivo were obtained by ultra-high-performance liquid chromatography-mass spectrometry.Moreover, the changes of the bioactive components in the blood were monitored over time (0-24 h) in order to identify stable active components. On this basis, the pharmacokinetic characteristics of these ingredients combined with the antiinflammatory activity were determined to screen out the potential markers for ensuring the quality control of Callerya nitida. The identified four components, such as calycosin, daidzein, formononetin, and 5-hydroxymethylfurfural, have the characteristics of intrinsic components, clearly defined structures, high exposure values, and in vivo stability, which are important for the therapeutic activity of pharmacologically active materials. Therefore, they can be used as potential markers to control the quality levels of Callerya nitida.

Quality evaluation methods of chinese medicine based on scientific supervision: recent research progress and prospects

“…hirsutissima was administered to rats, then blood samples were collected from the retroorbital venous plexus at 0.083, 0.5, 1.0, 1.5, 3.0, 4.0, 6.0, 8.0, 12.0 and 24.0 h for subsequent pharmacokinetic experiments. The values of area under the drug-time curve (AUC) and terminal elimination half-life (T1/2) in this experiment reflected that the chemical components were well absorbed and distributed in the body after oral administration [ 104 ]. Therefore, pharmacodynamics is one of the critical components for the quality control of CM, which lays a theoretical foundation for Q-markers and can be widely used in discovering Q-markers for CM to improve the quality control standards and clinical efficacy of CM.…”

Identification of pharmacokinetic markers for safflower injection using a combination of system pharmacology, multicomponent pharmacokinetics, and quantitative proteomics study

“…Feasible PK investigation strategies or methods for complex TCM products, such as, PK marker identification and integrated PK investigation, have been extensively investigated ( Shi et al, 2018 ; Chen et al, 2022 ). Among these studies, PK marker identification is considered a promising method for exploring the overall process of a complex TCM in vivo .…”