Screening for PVYN-Wi Resistance in Kenyan Potato Cultivars

Onditi, John; Nyongesa, Moses; Vlugt, R.A.A. van der

doi:10.1007/s11540-020-09488-4

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Potato virus Y (PVY) ranks among the most significant plant viruses that cause considerable damage all over the world. , Specifically, it damages the plants that belong to the family of Solanaceae − and induces mosaic, leaf drops, deformity, dwarfism, and necrosis on potato or tobacco. PVY is easily spread by common insects and mechanical contamination, which makes it difficult to control and prevent in agricultural activities all over the world. − …”

Section: Introductionmentioning

confidence: 99%

Synthesis of Planar Chiral Compounds Containing α-Amino Phosphonates for Antiplant Virus Applications against Potato Virus Y

Deng,

Cai,

Jin

et al. 2024

J. Agric. Food Chem.

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An unprecedented study of the application of planar chiral compounds in antiviral pesticide development is reported. A class of multifunctional planar chiral ferrocene derivatives bearing α-amino phosphonate moieties was synthesized. These compounds, exhibiting superior optical purities, were subsequently subjected to antiviral evaluations against the notable plant pathogen potato virus Y (PVY). The influence of the absolute configurations of the planar chiral compounds on their antiviral bioactivities was significant. A number of these enantiomerically enriched planar chiral molecules demonstrated superior anti-PVY activities. Specifically, compound (S p, R)-9n displayed extraordinary curative activities against PVY, with a 50% maximal effective concentration (EC50) of 216.11 μg/mL, surpassing the efficacy of ningnanmycin (NNM, 272.74 μg/mL). The protective activities of compound (S p, R)-9n had an EC50 value of 152.78 μg/mL, which was better than that of NNM (413.22 μg/mL). The molecular docking and defense enzyme activity tests were carried out using the planar chiral molecules bearing different absolute configurations to investigate the mechanism of their antiviral activities against PVY. (S p , R)-9n, (S p , R)-9o, and NMM all showed stronger affinities to the PVY-CP than the (R p , S)-9n. Investigations into the mechanisms revealed that the planar chiral configurations of the compounds played pivotal roles in the interactions between the PVY-CP molecules and could augment the activities of the defense enzymes. This study contributes substantial insights into the role of planar chirality in defending plants against viral infections.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Planar Chiral Compounds Containing α-Amino Phosphonates for Antiplant Virus Applications against Potato Virus Y

Deng,

Cai,

Jin

et al. 2024

J. Agric. Food Chem.

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“…Although significant efforts have been made to prevent and cure plant diseases caused by PVY infection, outcomes are far from satisfactory. For instance, the use of resistance cultivars has had a limited effect on alleviating potato leaf/tuber necrotic symptoms caused by PVY infections 11,12 . Traditional seed‐producing procedures can hardly figure out a breed that is prevent from tuber necrosis 13 .…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the use of resistance cultivars has had a limited effect on alleviating potato leaf/tuber necrotic symptoms caused by PVY infections. 11,12 Traditional seed-producing procedures can hardly figure out a breed that is prevent from tuber necrosis. 13 The use of conventional antiplant virus agents such as ribavirin and ningnanmycin (NNM) has had limited field control efficacies against PVY, 14,15 therefore, the development of effective methods for the control of PVY infection and transmission is of great importance and urgency.…”

Section: Introductionmentioning

confidence: 99%

Development of axially chiral urazole scaffolds for antiplant virus applications againstpotato virus Y

Jin

Mou

Zou

et al. 2023

Pest Management Science

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BACKGROUND Potato virus Y (PVY) was first discovered by Smith in 1931 and is currently ranked as the fifth most significant plant virus. It can cause severe damage to plants from the family Solanaceae, which results in billions of dollars of economic loss worldwide every year. To discover new antiviral drugs, a class of multifunctional urazole derivatives bearing a stereogenic CN axis were synthesized with excellent optical purities for antiviral evaluations against PVY. RESULTS The absolute configurations of the axially chiral compounds exhibited obvious distinctions in antiviral bioactivities, with several of these enantio‐enriched axially chiral molecules showing excellent anti‐PVY activities. In particular, compound (R)‐9f exhibited remarkable curative activities against PVY with a 50% maximal effective concentration (EC50) of 224.9 μg mL−1, which was better than that of ningnanmycin (NNM), which had an EC50 of 234.0 μg mL−1. And the EC50 value of the protective activities of compound (R)‐9f was 462.2 μg mL−1, which was comparable to that of NNM (442.0 μg mL−1). The mechanisms of two enantiomer of the axially chiral compounds 9f were studied by both molecule docking and defensive enzyme activity tests. CONCLUSION Mechanistic studies demonstrated that the axially chiral configurations of the compounds played significant roles in the molecule PVY‐CP (PVY Coat Protein) interactions and could enhance the activities of the defense enzymes. The (S)‐9f showed only one carbon–hydrogen bond and one π–cation interaction between the chiral molecule and the PVY‐CP amino acid sites. In contrast, the (R)‐enantiomer of 9f exhibited three hydrogen bonding interactions between the carbonyl groups and the PVY‐CP active sites of ARG157 and GLN158. The current study provides significant information on the roles that axial chiralities play in plant protection against viruses, which will facilitate the development of novel green pesticides bearing axial chiralities with excellent optical purities. © 2023 Society of Chemical Industry.

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Prevalence, distribution and control of potato virus Y (PVY) strains in Kenyan potato cultivars

Onditi

Nyongesa

Vlugt

2022

Trop. plant pathol.

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Potato virus Y (PVY) causes major yield losses in potato (Solanum tuberosum L.) crops worldwide. Over the past few decades, two new PVY strains (PVYN−Wi/N:O and PVYNTN) have become prevalent and widely distributed worldwide. In Kenya, there is limited information on the prevalence and distribution of PVY strains in potato cultivars grown in the farmer’s fields. This is a major limiting factor in making decisions for setting up a basis for PVY control. This study was therefore conducted (a) to determine the present prevalence and distribution of the different PVY strains in cultivars grown in different potato-growing counties and (b) to use this knowledge on virus prevalence and distribution in proposing a strategy for PVY control. To this end, a leaf sampling survey was conducted in 354 potato farms in five major potato-growing counties in Kenya with follow-up surveys in two of the five counties for two more seasons. Collected leaf samples were tested using DAS-ELISA, and those found to be infected with PVY were subjected to reverse transcription polymerase chain reactions (RT-PCR) to determine the PVY strains. Overall PVY prevalence was generally low (8.2%). Out of all the PVY-infected plants, PVYN−Wi/N:O was the most prevalent strain (54.9%) followed by PVYO (28.0%) and PVYNTN (17.1%), while PVYN was not detected. The PVYN−Wi/N:O strain occurred across the three seasons and across the counties but only on specific cultivars. Three cultivars were identified as PVY resistant, and the most widely grown cultivar Shangi (60.2%) had only a low PVY prevalence of 0.6%. This is an indication that this cultivar may have already been useful in minimizing virus prevalence in farmer’s fields. This report is the first prevalence survey of PVY strains and resistance; in addition, the results obtained provide a basis for a major focus on PVY resistance breeding in Kenya.

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Screening for PVYN-Wi Resistance in Kenyan Potato Cultivars

Cited by 3 publications

References 30 publications

Synthesis of Planar Chiral Compounds Containing α-Amino Phosphonates for Antiplant Virus Applications against Potato Virus Y

Synthesis of Planar Chiral Compounds Containing α-Amino Phosphonates for Antiplant Virus Applications against Potato Virus Y

Development of axially chiral urazole scaffolds for antiplant virus applications againstpotato virus Y

Prevalence, distribution and control of potato virus Y (PVY) strains in Kenyan potato cultivars

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