Screening for sweet potato (Ipomoea batatas L.) leaf curl virus (SPLCV) and its elimination using thermotherapy-meristem tip culture technique

Arkorful, Emmanuel; Appiah, AS; Dzahini-Obiatey, H.

doi:10.4038/jas.v10i1.8034

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Brown et al, (1997) also screened 12 clones with tester varieties for field resistance to potato leaf roll luteo virus. Similar results are given by Lakra (2003b), Lakra (2009), Arkorful et al, (2015), Manoj et al, (2015 and Saeed et al, (2017) for screening of potato progenies against leaf curl virus under natural field condition.…”

Section: Screening Of Bulks F 1 C 2 and F 1 C 3 Clonal Progenies Of Potato Against Leaf Curl Virussupporting

confidence: 84%

Identification of Promising Clones of Potato for Resistance against Leaf Curl Virus

Prabha¹,

Nanda²,

Sharma³

2019

Int.J.Curr.Microbiol.App.Sci

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Section: Screening Of Bulks F 1 C 2 and F 1 C 3 Clonal Progenies Of Potato Against Leaf Curl Virussupporting

confidence: 84%

Identification of Promising Clones of Potato for Resistance against Leaf Curl Virus

Prabha¹,

Nanda²,

Sharma³

2019

Int.J.Curr.Microbiol.App.Sci

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“…This combination proved moderately efficient on the vitroplants used in this work, since only 36 out of 351 generated meriplants (9.7%) were fully sanitized, representing 16 of the 57 accessions subjected to sanitation ( Table 4 ). Comparatively, 100% and 52.4% of sweet potato plantlets infected by sweet potato feathery mottle virus (SPFMV, genus Potyvirus ) and sweet potato leaf curl virus (SPLCV, genus Begomovirus ), respectively, were sanitized by the combination of thermotherapy and meristem excision [ 58 , 59 ]. The low rate of sanitation success in yam may result from the small size of yam meristems, which are particularly difficult to excise without surrounding contaminated cells, even following thermotherapy ( Figure 2 B).…”

Section: Discussionmentioning

confidence: 99%

Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange

Umber

Filloux

Gélabale

et al. 2020

Viruses

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Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.

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“…This process effectively removed RNA viruses from sweet potato propagation materials ( Wang and Valkonen, 2008 ). In contrast to RNA viruses, DNA viruses – in the case of SPLCV – were eliminated by about 50% through meristem tissue culture ( Arkorful et al, 2015 ; Cheong et al, 2010 ). Therefore, the DNA virus infection rate was not drastically decreased between 2012 and 2013.…”

Section: Discussionmentioning

confidence: 99%

Virus Incidence of Sweet Potato in Korea from 2011 to 2014

Kim

Yang

Kwak

et al. 2017

The Plant Pathology Journal

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A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Potyvirus species that infect sweet potato decreased between 2012 and 2013, and then increased again in 2014. Representatively, the incidence of Sweet potato feathery mottle virus (SPFMV) was 87.0% in 2012, 20.7% in 2013 and then increased to 35.3% in 2014. Unlike RNA viruses, DNA viruses were shown to decrease continuously. The incidence of Sweet potato leaf curl virus (SPLCV) was 5.5% in 2003, 59.5% in 2011, and 47.4% in 2012. It then decreased continuously year by year to 33.2% in 2013, and then 25.6% in 2014. While the infection rate of each virus species showed a tendency to decline, the virus infection status was more variable in 2013 and 2014. Nevertheless, the high rate of single infections and mixed infection combinations were more variable than the survey results from 2012. As shown in the results from 2013, the most prevalent virus infection was a single infection at 27.6%, with the highest rate of infection belonging to sweet potato symptomless virus-1 (SPSMV-1) (12.9%). Compared to 2013, infection combinations were more varied in 2014, with a total of 122 kinds of mixed infection.

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Screening for sweet potato (Ipomoea batatas L.) leaf curl virus (SPLCV) and its elimination using thermotherapy-meristem tip culture technique

Abstract: Despite its high potential for food security, production of sweet potato (Ipomoea batatas L.)

Cited by 4 publications

References 13 publications

Identification of Promising Clones of Potato for Resistance against Leaf Curl Virus

Identification of Promising Clones of Potato for Resistance against Leaf Curl Virus

Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange

Virus Incidence of Sweet Potato in Korea from 2011 to 2014

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