Screening for the antiplatelet aggregation quality markers of Salvia yunnanensis based on an integrated approach

Yang, Li; Zhang, Yanru; Cao, Bo; Zhang, Fengqin; Niu, Ming; Bai, Xue; Zhang, Mei; Zhang, Haizhu

doi:10.1016/j.jpba.2020.113383

Cited by 14 publications

(9 citation statements)

References 30 publications

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“…This is due to an imbalance in the concentrations of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) nucleotides and of adenosine (Ado), and nucleoside present in the tumor environment (TME), as well as overexpression or, in some cases, low expression of P2 receptors and ectonucleotidases [9][10][11] To corroborate the aforementioned conjectures, a large number of in vitro and in vivo studies have indicated the potential of phenolic compounds, especially caffeic acid (CA) and rosmarinic acid (RA), as antioxidant, antiproliferative, anticancerogenic, and antitumor agents, as well as modulators of cell pathways and biochemical cascades [12][13][14][15][16][17]. Furthermore, some studies have suggested the ability of RA to modulate the purinergic system [18].…”

Section: Highlightsmentioning

confidence: 99%

“…Finally, regarding platelet aggregation, there are studies that searched for the influence of RA on this process, such as Zou et al [155], who, through Danshen extract and having RA as active molecule, showed a potent inhibitory effect on the rats' platelets, aggregation induced by arachidonic acid, and that molecular docking thiol oxidoreductase enzyme (ERp57) is important for that process. Similarly, Salvia yunnanensis extract, which contains RA in its composition, inhibited ADP-induced rabbit platelet aggregation by binding rosmarinic acid with P2Y12R, cryptotanshinone, RA being fundamental in this outcome, which could be applied with antithrombotics [18].…”

Section: Anticancer and Antitumor Action Of Rosmarinic Acidmentioning

confidence: 99%

“…Although the P2Y1 and P2Y12 receptors act in the formation of platelets, only P2Y12 is related to thrombin and other platelet agonists and, therefore, is the target of another hypothesis of this study. They are located in platelets and in the central nervous system, especially in microglia [18].…”

Section: The Purinergic System Can Be the Target Of Rosmarinic Acid In A Pharmacological Therapeutic Perspective For Cutaneous Melanomamentioning

confidence: 99%

“…Thus, when analyzing the effects of the SY components, RA proved to be one of the main antiplatelet constituents in two ways: the first is the inhibitory effect of ERp57, a thiol oxidoreductase enzyme from the family of protein disulfide isomerase (PDI), which has an influence on platelet aggregation induced by arachidonic acid [155]. In addition, using molecular docking at the P2Y12 receptor, a high affinity between them and an ADP antagonistic effect was observed, which resulted in the inhibition of platelet aggregation [18].…”

Section: The Purinergic System Can Be the Target Of Rosmarinic Acid In A Pharmacological Therapeutic Perspective For Cutaneous Melanomamentioning

confidence: 99%

See 3 more Smart Citations

Novel possibility for cutaneous melanoma treatment by means of rosmarinic acid action on purinergic signaling

Silva

Yamauchi

Zanini

et al. 2021

Purinergic Signalling

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Section: Highlightsmentioning

confidence: 99%

Section: Anticancer and Antitumor Action Of Rosmarinic Acidmentioning

confidence: 99%

Section: The Purinergic System Can Be the Target Of Rosmarinic Acid In A Pharmacological Therapeutic Perspective For Cutaneous Melanomamentioning

confidence: 99%

Section: The Purinergic System Can Be the Target Of Rosmarinic Acid In A Pharmacological Therapeutic Perspective For Cutaneous Melanomamentioning

confidence: 99%

See 2 more Smart Citations

Novel possibility for cutaneous melanoma treatment by means of rosmarinic acid action on purinergic signaling

Silva

Yamauchi

Zanini

et al. 2021

Purinergic Signalling

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“…Recently, the concept of quality marker (Q-marker) has provided a new idea for the quality control of traditional Chinese medicine (TCM) (Tian et al, 2019), which is based on the perspective of biological activity, and through a variety of ways to find the chemical components that can reflect biological effect of TCM (Chen et al, 2021). Among them, the strategy to explore Q-marker from the perspective of the correlation between the chromatographic spectrums and biological effect (spectrum-effect) has been widely used in the study of various TCMs (Chen L. et al, 2020;Li et al, 2020;Jiang et al, 2021). A predictive analysis on the Q-marker of CMs was conducted by the review of its chemical composition and pharmacological effects (Zhou et al, 2019), however, the study to explore the Q-marker for the quality control of CM from the aspect of spectrum-effect relation has not been published to now.…”

Section: Introductionmentioning

confidence: 99%

Chemical Antioxidant Quality Markers of Chrysanthemum morifolium Using a Spectrum-Effect Approach

Zhang

et al. 2022

Front. Pharmacol.

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Traditionally, the quality evaluation of Chrysanthemum morifolium (CM) cv. (Juhua) attributes its habitats and processing methods, however, this strategy of neglecting bioactive ingredients usually results in deviation of quality evaluation. This study aims to explore the quality marker (Q-marker) based on spectrum-effect relationship and quality control strategy of CMs. The chromatographic fingerprint of 30 flower head samples of CMs from five different habitats including Hang-baiju, Gongju, Huaiju, Taiju and Boju were constructed by high performance liquid chromatography and analyzed through chemometrics methods such as similarity analysis (SA), cluster analysis (CA) and principal component analysis (PCA). The common peaks were quantified by external standard method and relative correction factor method. The in-vitro radical scavenging capacity assays of DPPH·, ·OH and ABTS were carried out. The Q-marker was explored by the correlation analysis between the contents of common peaks and in-vitro radical scavenging capacity, and then used to evaluate the quality of 30 flower head samples of CMs. A total of eight common peaks were appointed in 30 flower head samples of CMs, and their similarities ranged from 0.640 to 0.956. CA results showed that 30 flower head samples of CMs could be divided into five categories with reference to the Euclidean distance of 5. PCA results showed that common peaks played a major role in differential contribution of CMs. The quantification of common peaks hinted that their contents possessed significant variation whether for different accessions or the same accessions of CMs. The correlation analysis showed that chlorogenic acid, 3,5-O-dicaffeoylquinic acid, unknown peak 1, 4,5-O-dicaffeoylquinic acid and kaempferol-3-O-rutinoside could be used as the Q-markers for the quality evaluation of 30 flower head samples of commercially available CMs. The analysis strategy that combines chromatographic fingerprint analysis, multiple ingredients quantification, in-vitro chemical anti-oxidant activity evaluation and spectrum-effect relationship analysis clarified the therapeutic material basis and discovered the Q-markers, which possibly offers a more comprehensive quality assessment of CMs.

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Multimodal integrated strategy for the discovery and identification of antiplatelet aggregation Q‐markers in Paris polyphylla var. yunnanensis

Li,

Wen,

Yang

et al. 2024

Biomedical Chromatography

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To enhance the quality evaluation and control of traditional Chinese medicine (TCM) and ensure the safety and efficacy of clinical medication, it is imperative to establish a comprehensive quality assessment method aligned with TCM efficacy. This study uses a representative Chinese medicine with multi‐origin and multi‐efficacy, Paris polyphylla var. yunnanensis (PY), as an illustrative example. Surprisingly, despite the high fingerprint similarity among the 12 batches of PY samples collected from various regions in Yunnan, a notable variation in the composition and content of components was observed. The chromatographic analysis identified seven common peaks, namely, polyphyllin I, polyphyllin II, polyphyllin V, polyphyllin VI, polyphyllin VII, polyphyllin H, and polyphyllin D. In the bioactivity evaluation, an in vitro antiplatelet aggregation model induced by adenosine diphosphate was established, showcasing excellent stability. The maximum antiplatelet aggregation inhibition rate for all PY samples consistently remained stable at 73.1%–99.1%. However, the 50% inhibitory concentration (IC50) values exhibited a range from 1.615 to 18.200 mg/mL. This approach not only meets high‐throughput screening requirements but also demonstrates remarkable discrimination. The results of chemical and bioactivity evaluations were analyzed using hierarchical cluster analysis and canonical correlation analysis. Polyphyllin I, polyphyllin II, polyphyllin VII, polyphyllin H, and polyphyllin D were identified as the Q‐markers for antiplatelet aggregation in PY samples. Validation of the bioactivity for these monomer components aligned with the previously mentioned findings. Notably, this study established a spectrum–effect model for PY samples, enhancing the scientific robustness of the quality evaluation method. Furthermore, these findings offer valuable research insights for improving the quality assessment of other TCMs.

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Screening for the antiplatelet aggregation quality markers of Salvia yunnanensis based on an integrated approach

Cited by 14 publications

References 30 publications

Novel possibility for cutaneous melanoma treatment by means of rosmarinic acid action on purinergic signaling

Novel possibility for cutaneous melanoma treatment by means of rosmarinic acid action on purinergic signaling

Chemical Antioxidant Quality Markers of Chrysanthemum morifolium Using a Spectrum-Effect Approach

Multimodal integrated strategy for the discovery and identification of antiplatelet aggregation Q‐markers in Paris polyphylla var. yunnanensis

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