Screening For Yeast Phytase Leads to the Identification of a New Cell-Bound and Secreted Activity in Cyberlindnera jadinii CJ2

Capusoni, Claudia; Serra, Immacolata; Donzella, Silvia; Compagno, Concetta

doi:10.3389/fbioe.2021.662598

Cited by 8 publications

(7 citation statements)

References 42 publications

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“…The optimum temperature for phytase activity in Cyberlindnera jadinii CJ2 was found to be 50°C with a highest activity of 51.95 mU/mgdw, while the activity decreased with increase in temperature (24). Capusoni et al, (24) have shown pH 4.5 as optimum for highest phytase activity by Cyberlindnera jadinii CJ2. According to Alves et al, (25) Muscodor sp.…”

Section: Discussionmentioning

confidence: 93%

“…In another study, 50°C was shown to be an appropriate temperature for a bacterial strain named DR 6 to produce 387 U/ml of phytase (9). The optimum temperature for phytase activity in Cyberlindnera jadinii CJ2 was found to be 50°C with a highest activity of 51.95 mU/mgdw, while the activity decreased with increase in temperature (24). Capusoni et al, (24) have shown pH 4.5 as optimum for highest phytase activity by Cyberlindnera jadinii CJ2.…”

Section: Discussionmentioning

confidence: 94%

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Isolation, characterization and optimization of Cystobasidium minutum for phytase production

Gowthami¹,

Gunashree²

2023

Biomedicine

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Introduction and Aim: Phytic acid (myo-inositol 1, 2, 3, 4, 5, 6- hexakis dihydrogen phosphate), a storage form of phosphorus is present in legumes, cereals and oilseeds. By forming insoluble complexes with proteins and divalent cations, it serves as an anti-nutrient in animal feed. Phytase (EC 3.1.3.8), a special class of phosphomonoesterases, catalyse the conversion of inorganic phosphate into mono-, di-, tri-, tetra-, and penta-phosphates of derivatives of myo-inositol. It is an important enzyme in food and feed industries. The present study aimed at isolation, molecular identification and optimization of physico-chemical parameters for phytase production by a red pigmented yeast Cystobasidium minutum. Materials and Methods: The phytase activity was estimated by using sodium phytate as substrate and the production of phytase under varied temperature, pH, agitation, incubation time, carbon and nitrogen sources. Results: The results showed maximum activity of 91.86 and 27.13 U/ml at 35°C and pH 5.5. At an agitation speed of 150 rpm and 120h of incubation time the enzyme activity was 18.9 and 21.4 U/ml respectively. Among the carbon sources tested sucrose served for highest enzyme activity of 26.33 U/ml and ammonium sulphate served the sole source of nitrogen and showed an activity of 50.12 U/ml. Conclusion: Cystobasidium minutum (Rhodotorula minutum) produced maximum phytase enzyme in an optimized physical and chemical condition. Hence, from the present investigation, it is found that optimization of physic-chemical conditions may be a promising tool for the growth of organisms and also maximum yield of any metabolite.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 94%

Isolation, characterization and optimization of Cystobasidium minutum for phytase production

Gowthami¹,

Gunashree²

2023

Biomedicine

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“…Cyberlindnera jadinii CJ2 Cell bound Capusoni et al 96 Finarome sp. Cell bound Klosowski et al 97 Pichia kudriavzevii Cell bound Hellstrom et al 98 Pseudomonas strains Cell bound Zhuang et al 99 Bacillus subtilis P6 Intracellular Trivedi et al 100 Talaromyces purpureogenus NSA20 Intracellular Ahmed et al 101 Pediococcus acidilactici SMVDUDB2 Intracellular Bhagat et al 102 Pseudomonas mandelii GS10-1 Extracellular Li et al 103 Bacillus licheniformis Extracellular Dan et al 104 Pediococcus pentosaceus KTU05-8, KTU05-9 Extracellular Cizeikiene et al 105 Lactic acid bacteria Extracellular Nuobariene et al 106 Lactobacillus fermentum spp.…”

Section: Microorganisms Site Referencesmentioning

confidence: 99%

Unraveling the potential of bacterial phytases for sustainable management of phosphorous

Vashishth

Tehri

Tehri³

et al. 2023

Biotech and App Biochem

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Phosphorous actively participates in numerous metabolic and regulatory activities of almost all living organisms including animals and humans. Therefore, it is considered as an essential macronutrient required supporting their proper growth. On contrary, phytic acid (PA), an antinutritional substance, is widely known for its strong affinity to chelate essential mineral ions including PO43−, Ca2+, Fe2+, Mg2+, and Zn2+. Being one the major reservoir of PO43− ions, PA has great potential to bind PO43− ions in diverse range of foods. Once combined with P, PA transforms into an undigested and insoluble complex namely phytate. Produced phytate leads to a notable reduction in the bioavailability of P due to negligible activity of phytases in monogastric animals and humans. This highlights the importance and consequent need of enhancement of phytase level in these life forms. Interestingly, phytases, catalyzing the breakdown of phytate complex and recycling the phosphate into ecosystem to its available form, have naturally been reported in a variety of plants and microorganisms over past few decades. In pursuit of a reliable solution, the focus of this review is to explore the keynote potential of bacterial phytases for sustainable management of phosphorous via efficient utilization of soil phytate. The core of the review covers detailed discussion on bacterial phytases along with their widely reported applications viz. biofertilizers, phosphorus acquisition, and plant growth promotion. Moreover, meticulous description on fermentation‐based strategies and future trends on bacterial phytases have also been included.

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“…D. hansenii is able to grow using phytic acid as a sole phosphorus source [45]. To characterize phytase activity in the Mo40 strain, we started sequencing the gene encoding in order to compare a marine phytase sequence with its terrestrial counterpart (reference strain CBS 767, isolated from beer [46]).…”

Section: Phytase Activitymentioning

confidence: 99%

Bioprocesses with Reduced Ecological Footprint by Marine Debaryomyces hansenii Strain for Potential Applications in Circular Economy

Donzella

Capusoni

Pellegrino

et al. 2021

JoF

Self Cite

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The possibility to perform bioprocesses with reduced ecological footprint to produce natural compounds and catalyzers of industrial interest is pushing the research for salt tolerant microorganisms able to grow on seawater-based media and able to use a wide range of nutrients coming from waste. In this study we focused our attention on a Debaryomyces hansenii marine strain (Mo40). We optimized cultivation in a bioreactor at low pH on seawater-based media containing a mixture of sugars (glucose and xylose) and urea. Under these conditions the strain exhibited high growth rate and biomass yield. In addition, we characterized potential applications of this yeast biomass in food/feed industry. We show that Mo40 can produce a biomass containing 45% proteins and 20% lipids. This strain is also able to degrade phytic acid by a cell-bound phytase activity. These features represent an appealing starting point for obtaining D. hansenii biomass in a cheap and environmentally friendly way, and for potential use as an additive or to replace unsustainable ingredients in the feed or food industries, as this species is included in the QPS EFSA list (Quality Presumption as Safe—European Food Safety Authority).

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Screening For Yeast Phytase Leads to the Identification of a New Cell-Bound and Secreted Activity in Cyberlindnera jadinii CJ2

Cited by 8 publications

References 42 publications

Isolation, characterization and optimization of Cystobasidium minutum for phytase production

Isolation, characterization and optimization of Cystobasidium minutum for phytase production

Unraveling the potential of bacterial phytases for sustainable management of phosphorous

Bioprocesses with Reduced Ecological Footprint by Marine Debaryomyces hansenii Strain for Potential Applications in Circular Economy

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