Screening Lactobacillus strains from artisanal Turkish goatskin casing Tulum cheeses produced by nomads via molecular and in vitro probiotic characteristics

Abstract

BACKGROUND Eleven Lactobacillus (L.) strains were newly isolated from traditional Turkish Tulum cheeses and were characterized regarding their potential probiotic characteristics (bile and acid tolerance, gastric and pancreatic juice tolerance, lysozyme tolerance, adhesion ability), virulence determinants (hemolytic activity, antibiotic resistance, biogenic amine production), and functional properties (antibacterial activity, β‐galactosidase activity, production of exopolysaccharides, cholesterol removal). RESULTS These isolates were identified as L. brevis, L. plantarum, L. paracasei, L. coryniformis, L. rhamnosus and L. helveticus by 16S rRNA sequencing. With regard to safety aspects, none of the tested Lactobacillus isolates showed hemolytic activity or biogenic amine production. All the Lactobacillus isolates except isolate 24 were found to be sensitive or intermediate sensitive to penicillin, which is a frequently used antibiotic. Nine Lactobacillus isolates showed antibacterial activity against Staphylococcus aureus ATCC 25923, while only isolates 15 and 449 exhibited inhibitory activity against Listeria monocytogenes ATCC 7644. All isolated strains survived, even in the presence of 10.00 g L−1 bile after 48 h, and exhibited good survival at pH 3, but only two isolates survived at pH 2. Among the strains, isolate 15 exhibited satisfactory auto‐aggregative, cell‐surface hydrophobicity features, cholesterol‐lowering activity and good acid tolerance. Isolate 15 also showed the strongest bile and simulated pancreatic juice resistance and moderate lysozyme tolerance. CONCLUSION These outcomes suggest that isolate 15, identified as a L. plantarum strain from Tulum cheese, may be a promising probiotic candidate and could be suitable for use in several fermented foods. © 2020 Society of Chemical Industry

Sheep Milk Symbiotic Ice Cream: Effect of Inulin and Apple Fiber on the Survival of Five Probiotic Bacterial Strains during Simulated In Vitro Digestion Conditions

“…Bile secreted into the digestive tract plays an important role in emulsifying lipids, especially since the fat content in the ice cream evaluated ranged from 6.2% to 6.4%. Furthermore, bile has the ability to affect phospholipids and cell membrane proteins and thus disrupt the cellular homeostasis of microorganisms [ 73 , 74 ]. Bile salts exhibit potent antimicrobial activity by changing the conformation of cell membrane proteins and lipids, resulting in altered membrane integrity and permeability.…”

Functional Foods, Nutraceuticals and Probiotics: A Focus on Human Health

“…Artisanal cheeses from various regions of Mexico were studied by Özkan et al [ 31 ]. This group screened lactobacilli strains from artisanal cheeses from Tulum (through molecular and in vitro probiotic characteristics), which had been prepared by covering them with Turkish goatskin produced by nomads.…”

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

“…Cultures showing good lactose utilizing ability were selected for the determination of β-galactosidase activity using the method describes by Özkan et al [6] with several modifications. For this purpose, absorbance of activated cultures was taken at 600 nm and then 20 µl of 24 h grown cultures were mixed with 80 µl of permeabilization solution (100 mM Na 2 HPO 4 , 20 mM KCL, 2 mM MgSO 4 , 0.8 mg/ml CTAB, 0.4 mg/ml SDS, and 5.4 µl/ml β-mercaptoethanol) followed by incubation at 37°C for 20-30 min.…”

“…Many children and adults are unable to digest lactose due to insufficient production of β-galactosidase enzyme and suffer from lactose intolerance which is a common digestive problem when person consume milk or any other dairy products [38]. Probiotic organisms have been found beneficial in improving these symptoms through the production of β-galactosidase [6]. In this study, all lactose utilizing probiotic isolates were screened for β-galactosidase activity by ONPG method.…”

“…Probiotics also possess some special properties such as anti-inflammatory, anti-oxidant, anti-diabetic, anti-cancer, cholesterol removal, and vitamin production [5]. Indeed, several studies have validated that probiotics have advantageous effects on a wide range of disorders such as irritable bowel syndrome, inflammatory bowel disease, diarrhea, constipation, hypertension, lactose intolerance, and hypersensitivity [6].…”