Screening of a microbial consortium with efficient corn stover degradation ability at low temperature

Borjigin, Qinggeer; Gao, Julin; Yu, Xiang; Zhang, Baolin; Wang, Zhigang; Borjigin, Naoganchaolu; Hu, Shuping; Sun, Jiying; Xie, Min; Wang, Zhen

doi:10.1016/s2095-3119(15)61272-2

Cited by 17 publications

(8 citation statements)

References 34 publications

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“…The microbial consortium GF-20 used in this study was obtained through low-temperature acclimation in lowtemperature in our lab in 2016. The microbial consortium GF-20 had stable corn stover decomposing activity, it could significantly promote corn stover decompositionrelating capacity at low temperature (4 °C-10 °C) (Qinggeer et al, 2016).…”

Section: Sample Informationmentioning

confidence: 99%

“…To estimate enzyme activities of FPA, Xylanase and Laccase activity, the microbial consortium (5%, v/v) inoculated on corn stover cotaining medium at 15 °C under shaking conditions cultivated 30 d. The activities of FPA, Xylanase and Laccase were assayed using the techniques as previous described (Qinggeer et al, 2016;Wolfenden & Willson, 1982).…”

Section: Analysis Of Fpa Xylanase and Laccase Activity In The Microbi...mentioning

confidence: 99%

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Metagenomic Analysis of Microbial Consortium Gf-20 in Corn Stover Degradation at Low Temperature

Zhang

Borjigin

Gao

et al. 2023

Journal of Environmental Engineering and Landscape Management

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In our previous work, a microbial consortium GF-20 (Qinggeer et al., 2016) was enriched from compost habitats and adapted to efficiently and stably degrade corn stover under low temperatures. While the main microorganism and degradation-related functions and degradation-related coding enzyme genes of GF-20 were not clear. Therefore, the current study used the metagenomic to decipher the systematic and functional contexts within such microbial consortium under low temperatures. The results showed that the dominant functional microbials in GF-20 consortium were bacteria. The dominant phylums in GF-20 consortium were Proteobacteria (62.84%) and Bacteroidetes (10.24%). The dominant genus was Pseudomonas (50.84%), followed by Dysgonomonas (5.86%), Achromobacter (4.94%), Stenotrophomonas (3.67%) and Flavobacterium (2.04%). The metabolism was mainly composed of carbohydrate metabolism and amino acid metabolism, and included signal transduction, cell transport and other metabolic modes. The functional genes encoded were mainly distributed in glycosidolytic enzyme genes, and the functional enzymes were β-glucosidase, acetyl-CoA, pyruvate dehydrogenase and galactosidase. The GF-20 microbial consortium degraded the cellulose in corn stover primarily by β-glucosidase and endoglucanase, which were produced by 12 genera of microorganisms. The hemicellulose synergistic effect was produced by 15 genera of microorganisms including xylanase, xyloglucanase, mannolanase and branching enzyme.

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Section: Sample Informationmentioning

confidence: 99%

Section: Analysis Of Fpa Xylanase and Laccase Activity In The Microbi...mentioning

confidence: 99%

Metagenomic Analysis of Microbial Consortium Gf-20 in Corn Stover Degradation at Low Temperature

Zhang

Borjigin

Gao

et al. 2023

Journal of Environmental Engineering and Landscape Management

Self Cite

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“…The degradation rate of stone cells was determined using the weight method (Qinggeer et al, 2016), The degradation rate of stone cells fermented by L. plantarum JYLP-326 alone and mixed-culture fermentation by L. plantarum JYLP-326 and S. cerevisiae Y2 for 96 h was determined by weight method. The sample preparation method is the same as 2.3, and the degradation rate of stone cells was calculated as follows.…”

Section: Determination Of the Degradation Rate Of Stone Cells After I...mentioning

confidence: 99%

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

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“…In China, as an important source of lignin, crop straw is abundant, with a yield of 800 million tons annually, including approximately 222 million tons of corn straw output ( Yang et al, 2016 ). The yield of corn straw in northeast China accounts for 31% that in the whole country, reaching 68.5 million tons ( Qinggeer et al, 2016 ). However, due to the high latitude, long winter, 5–6 months of freezing conditions and low temperatures ( He et al, 2009 ), most of the corn straw is burned, limiting the implementation of better treatment strategies such as straw return to the field, resulting in not only severe air pollution but also the waste of lignin resources ( Yang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

et al. 2022

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In the cold regions of China, lignin-rich corn straw accumulates at high levels due to low temperatures. The application of psychrotrophic lignin-degrading bacteria should be an effective means of overcoming the low-temperature limit for lignin degradation and promoting the utilization of corn straw. However, this application is limited by the lack of suitable strains for decomposition of lignin; furthermore, the metabolic mechanism of psychrotrophic lignin-degrading bacteria is unclear. Here, the whole genome of the psychrotrophic lignin-degrading bacterium Arthrobacter sp. C2, isolated in our previous work, was sequenced. Comparative genomics revealed that C2 contained unique genes related to lignin degradation and low-temperature adaptability. DyP may participate in lignin degradation and may be a cold-adapted enzyme. Moreover, DyP was proven to catalyze lignin Cα-Cβ bond cleavage. Deletion and complementation of the DyP gene verified its ability to catalyze the first-step reaction of lignin degradation. Comparative transcriptomic analysis revealed that the transcriptional expression of the DyP gene was upregulated, and the genetic compensation mechanism allowed C2ΔDyP to degrade lignin, which provided novel insights into the survival strategy of the psychrotrophic mutant strain C2ΔdyP. This study improved our understanding of the metabolic mechanism of psychrotrophic lignin-degrading bacteria and provided potential application options for energy-saving production using cold-adapted lignin-degrading enzymes.

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Screening of a microbial consortium with efficient corn stover degradation ability at low temperature

Cited by 17 publications

References 34 publications

Metagenomic Analysis of Microbial Consortium Gf-20 in Corn Stover Degradation at Low Temperature

Metagenomic Analysis of Microbial Consortium Gf-20 in Corn Stover Degradation at Low Temperature

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Genome Functional Analysis of the Psychrotrophic Lignin-Degrading Bacterium Arthrobacter sp. C2 and the Role of DyP in Catalyzing Lignin Degradation

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