Screening of agro-industrial waste and physical factors for the optimum production of pullulanase in solid-state fermentation from endophytic Aspergillus sp.

Naik, Bindu; Goyal, Surbhi; Tripathi, Avanish; Kumar, Vijay

doi:10.1016/j.bcab.2019.101423

Cited by 28 publications

(7 citation statements)

References 31 publications

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“…Todas as culturas de Aspergillus ochraceus URM604 foram capazes de crescer nas condições avaliadas, da mesma forma todos os extratos apresentaram um alto teor de proteínas e proteases. Estes substratos já foram utilizados por vários autores, o farelo de trigo é frequentemente relatado na literatura para produção de enzimas, Nascimento et al (2017) apresenta farelo de trigo como melhor substrato na produção de proteases com ação fibrinolítica a partir da fermentação em estado sólido com fungos filamentosos; Naik et al (2019) obteve o mesmo resultado ao comparar farelo de trigo com resíduos agroindustriais na produção de enzimas por Aspergillus sp. O uso de resíduos de café é pouco mencionado na literatura embora apresente grande potencial (Cerda, et al, 2017), ainda assim foram utilizados em estudos para produção de celulases e xilanases por bactérias (Pseudoxanthonomas taiwanensis e Sphingobacterium composti) e leveduras (Cyberlindnera jardinii e Barnettozyma californica), demonstrando serem excelentes meios para o cultivo de microrganismos (Soccol et al, 2017).…”

Section: Resultsunclassified

Protease com atividade fibrinolítica e colagenolítica produzida por Aspergillus ochraceus URM604

Cardoso

Nascimento

Oliveira

et al. 2022

RSD

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Proteases possuem um papel fundamental na economia, estando presentes em diversos setores industriais. Dentre os fungos conhecidos por produzir proteases, destaca-se o gênero Aspergillus. Desta forma, o objetivo deste trabalho foi produzir proteases a partir de fermentação em estado sólido de Aspergillus ochraceus, purificar, caracterizar e avaliar seu potencial na produção de enzima colagenolítica e fibrinolítica. Para isto foram avaliados o tipo e quantidade do substrato e a umidade das fermentações. Posteriormente foi realizada purificação por cromatografia DEA-Sephadex e Superdex-G75 em sistema FPLC. Assim como caracterização parcial avaliando o pH e temperatura ótimos da ação da protease. Foi observada que a melhor atividade da enzima nas fermentações com farelo de trigo como substrato (3g) e 60% de umidade, apresentando atividade proteásica, colagenolítica e fibrinolítica. Também foi observada maior atividade em pH8 e 50°C. Assim, concluísse que A. ochraceus possui grande potencial para aplicação biotecnológica, destacando-se a importância de futuras avaliações.

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Section: Resultsunclassified

Protease com atividade fibrinolítica e colagenolítica produzida por Aspergillus ochraceus URM604

Cardoso

Nascimento

Oliveira

et al. 2022

RSD

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“…Salts such as 0.2% MgSO 4 , 1% KH 2 PO 4, 0.2% NaCl, and 1% (NH 4 ) 2 SO 4 , were added to 5 g of each substrate. The inoculum of 6.42 log CFU/gds 7 (colony-forming unit/gram dry substrate) was added to each flask with a final moisture content of 69.9%. All the flasks were then autoclaved at 121 °C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Agro-waste as a substrate for the production of pullulanase by Penicillium viridicatum under solid-state fermentation

Kumar

Naik

Choudhary

et al. 2022

Sci Rep

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One of the key enzymes utilized in the food industry is pullulanase. But its major drawbacks are its low yield and high production costs. In this regard, the current research aims to screen agro-waste substrates for optimal pullulanase production in solid-state fermentation. Of various agro-wastes used as a substrate, the maximum enzymic activity (9.74 U/gds) was observed in a medium based on 5 g of green gram husk and incubated for 3 days at 30 °C. The effects of 16 different nutrients on the yield of pullulanase production were studied using the Plackett–Burman experimental design. The incorporation of FeSO4, MnSO4, and MgSO4 into the pullulanase production medium significantly increased the yield and showed a 5.7-fold increase (56.25 U/gds) in comparison with the unoptimized media. The Box-Behnken experimental design was used to study the effect of interactions between Fe2+, Mg2+, and Mn2+ on the production of pullulanase. Box-Behnken showed a 1.1-fold increase (62.1 U/gds) in pullulanase production. The total increase in yield after all optimization was 6.37-fold. The present study reports for the first time the applicability of green gram husk as a potent substrate for pullulanase production by Penicillium viridicatum.

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“…Aspergillus sp . [ 42 ], although this enzyme was first discovered in bacteria by Bender and Wallenfels in 1961 [ 43 ]. The genus of Aspergillus is also capable of producing cellulase [ 44 ], glucose oxidase, pectinase [ 45 ], mannanase [ 46 ], lipase [ 47 ], pectinase [ 48 ], and other types of enzymes.…”

Section: Enzyme Technologymentioning

confidence: 99%

Role of ascomycete and basidiomycete fungi in meeting established and emerging sustainability opportunities: a review

et al. 2022

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Fungal biomass is the future’s feedstock. Non-septate Ascomycetes and septate Basidiomycetes, famously known as mushrooms, are sources of fungal biomass. Fungal biomass, which on averagely comprises about 34% protein and 45% carbohydrate, can be cultivated in bioreactors to produce affordable, safe, nontoxic, and consistent biomass quality. Fungal-based technologies are seen as attractive, safer alternatives, either substituting or complementing the existing standard technology. Water and wastewater treatment, food and feed, green technology, innovative designs in buildings, enzyme technology, potential health benefits, and wealth production are the key sectors that successfully reported high-efficiency performances of fungal applications. This paper reviews the latest technical know-how, methods, and performance of fungal adaptation in those sectors. Excellent performance was reported indicating high potential for fungi utilization, particularly in the sectors, yet to be utilized and improved on the existing fungal-based applications. The expansion of fungal biomass in the industrial-scale application for the sustainability of earth and human well-being is in line with the United Nations’ Sustainable Development Goals.

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Screening of agro-industrial waste and physical factors for the optimum production of pullulanase in solid-state fermentation from endophytic Aspergillus sp.

Cited by 28 publications

References 31 publications

Protease com atividade fibrinolítica e colagenolítica produzida por Aspergillus ochraceus URM604

Protease com atividade fibrinolítica e colagenolítica produzida por Aspergillus ochraceus URM604

Agro-waste as a substrate for the production of pullulanase by Penicillium viridicatum under solid-state fermentation

Role of ascomycete and basidiomycete fungi in meeting established and emerging sustainability opportunities: a review

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