Screening of amylolytic and cellulolytic yeast from Dendrobium spathilingue in Bali Botanical Garden, Indonesia

Arman, Zico; Sondana, G. A.; Fikriyyah, N. N.; Afifah, Z. N.; Balqis, M.; Hasanah, Reni Wulan; Risandi, A.; Sofiana, Indah; Nisa, Humeera; Ridawati,; Muktiningsih, M; Sukmawati, Dalia

doi:10.1063/5.0007802

Cited by 7 publications

(4 citation statements)

References 8 publications

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“…All other strains exhibited low and moderate lipolytic activity. The clear zone around the colony showed that the isolates were also capable of promoting cellulase hydrolysis [48] . Among the 78 yeast strains, most had no cellulase activity except five strains, namelyPO27, PO6, PO8, PO20, and RW2-2, which all showed weak activities ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast Geotrichum candidum PO27

Chaib,

Dakhmouche-Djekrif,

Bennamoun

et al. 2024

AIMSMICRO

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<abstract> Enzymes are biocatalysts mainly used for their industrial potential in various applications. The present study aims to understand the enzyme production for biotechnological interest from a local yeast strain. From 100 isolates obtained from various biotopes, 78 strains were selected for their enzymatic heritage. Screening of α-amylase, lipase/esterase, and cellulase activities by rapid plate detection methods was carried out and the PO27 yeast was selected for its high capacity to produce α-amylase. In addition, this yeast strain exhibited good lipolytic and esterolytic activities, as well as low cellulase activity. A sequence analysis of the D1/D2 region of the 26S ribosomal RNA (26S rRNA) and a study of morphological characteristics identified the PO27 strain as <italic>Geotrichum candidum</italic>. The production of α-amylase has been studied in solid medium fermentation using various natural substrates without any supplementation such as olive pomace, potato peels, leftover bread, and mastic cake. <italic>G. candidum</italic> PO27 showed an improved production of α-amylase with olive pomace, thus reaching approximately 180.71 U/g. To evaluate the ability of this isolate to produce α-amylase in submerged fermentation, multiple concentrations of olive pomace substrate were tested. The best activity of submerged fermentation was statistically compared to the solid-state fermentation result in order to select the appropriate fermentation type. A high significant difference was found to rank the 6% olive pomace medium as the best substrate concentration with 34.395 U/mL of α-amylase activity. This work showed that the new isolate <italic>Geotrichum candidum</italic> PO27 has a better potential to produce α-amylase at a low cost in solid-state fermentation compared to submerged fermentation. Optimization conditions for PO27 α-amylase production through solid-state fermentation were achieved ‎ using a one factor at a time (OFAT) approach. The findings revealed that a high temperature (60 °C), an acidic pH, malt extract, and soluble starch were the highly significant medium components for enhancing α-‎amylase production. The use of olive pomace waste by <italic>Geotrichum candidum</italic> PO27 is expected to be effective in producing an industrially useful α-amylase. </abstract>

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Section: Resultsmentioning

confidence: 99%

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast Geotrichum candidum PO27

Chaib,

Dakhmouche-Djekrif,

Bennamoun

et al. 2024

AIMSMICRO

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“…Clear zones on the CMC media were a result of the cleavages of β-1,4glycosidic bonds from the CMC in the media, due to the presence of CMCase and the presences of strong bonds of the β-(1,4)-D glucopyranose containing polysaccharides with the congo red (Sukmawati et al, 2018). The differences in cellulolytic indexes between isolates is an indication that each isolate has its own unique capacity in producing cellulase (Arman et al, 2020). Following are the clear zones of the isolates on selective CMC media, indicating they have lignocellulolytic potential (Figure 2).…”

Section: Cellulolytic Activitymentioning

confidence: 99%

Isolation and Molecular Screening of Fungus as Agents in Cellulolytic Transformation Materials from Symbiotic Lichen

Rahardiyan,

Moko

2023

J Bio Bio Edu

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The abundance of lignocellulosic waste makes it a potential source for advanced biomaterials through various transformation processes. Lignocellulosic biomass transformation to advanced biomaterials involves enzymes from extracellular metabolites of microorganisms capable of hydrolyzing lignocellulose. This research was to molecularly screen fungi found in symbiotes of lichen endemic to trees growing in North Minahasa (North Sulawesi) with lignocellulolytic transformation enzymes. Molecular screening was conducted from identified fungi isolates based on partial genetical analysis on the locus of Internal Transcribed Spacer (ITS) of the fungi’s ribosomal DNA. Fungi isolates screening identified Trichoderma koningiopsis (isolate HZA8 and isolate HZA6), Penicillium sumatraense (strain CBS 127365 and strain CBS 130380), Trichoderma hamatum (isolate PAN12-45 and isolate PAN12-05), Aspergillus aculeatus (strain A1.9 18S), Aspergillus aculeatus (isolate XSD-74), Trichoderma reesei (strain S2606 and isolate 5A14). Molecular identification and BLAST homology of potentially lignocellulolytic fungi isolates rDNA indicated that isolate KB2 had close relationship with Trichoderma reesei at 100% degree of closeness and an index of cellulolytic activity of 1.19. While isolate KB3 appeared closely related to Aspergillus aculeatus at 99.83% degree of closeness and an index of cellulolytic activity of 1.57. Therefore, the potential of developing bioprocess industries in general and in particular is most probable.

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“…Penelitian Sari et al (2017) menunjukkan bahwa hasil uji aktivitas selulolitik jamur hasil isolasi dari limbah pengolahan sagu menunjukkan indeks selulolitik tertinggi sebesar 2,63. Indeks selulolitik yang dihitung memiliki nilai yang berbeda antar isolat menunjukkan bahwa terdapat perbedaan kemampuan masing-masing isolat jamur dalam menghasilkan selulase untuk hidrolisis selulosa (Arman et al, 2020).…”

Section: Pembahasanunclassified

Isolasi dan Karakterisasi Jamur Selulolitik pada Fermetodege: Pakan Fermentasi Berbahan Campuran Eceng Gondok, Bekatul Padi, dan Tongkol Jagung

Hidayat

Isnawati

2021

LenteraBio

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Jamur selulolitik berperan dalam proses degradasi bahan yang mengandung selulosa seperti eceng gondok, bekatul padi, dan tongkol jagung yang merupakan campuran bahan pakan fermentasi. Penelitian ini bertujuan memperoleh isolat jamur selulolitik dan mendeskripsikan karakterisitik isolat jamur yang diisolasi dari fermetodege: pakan fermentasi berbahan campuran eceng gondok, bekatul padi, dan tongkol jagung berdasarkan aktivitas selulolitik isolat jamur yang mampu mendegradasi media carboxymethyl cellulose (CMC). Isolasi jamur menggunakan media potato dextrose agar (PDA) dengan metode pour plate. Isolat jamur diseleksi dengan menumbuhkan di media CMC dan diamati terbentuknya zona bening di sekitar koloni setelah diwarnai dengan Congo red 0,1%. Indeks selulolitik isolat jamur dihitung berdasarkan perbandingan antara diameter zona bening dan diameter koloni jamur. Karakterisasi dilakukan dengan pengamatan morfologi koloni secara makroskopis serta pengamatan mikroskopis. Hasil isolasi diperoleh 9 isolat jamur selulolitik dengan 3 isolat jamur memiliki indeks selulolitik berkategori sedang (1<indeks<2) yaitu JPS1, JPS2, dan JPS9, sementara 6 isolat jamur dengan indeks selulolitik kategori rendah (indeks<1) antara lain JPS3, JPS4, JPS5, JPS6, JPS7, dan JPS8. Kesembilan isolat jamur selulolitik dikarakterisasi termasuk genus Aspergillus (JPS1, JPS2, JPS5, JPS7, JPS8, dan JPS9), Mucor (JPS3), Rhizopus (JPS4), dan Penicillium (JPS6). Isolat jamur selulolitik yang diperoleh dalam penelitian ini berpotensi dikembangkan lebih lanjut sebagai kandidat inokulum pada proses fermentasi bahan dengan kandungan selulosa.

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Screening of amylolytic and cellulolytic yeast from Dendrobium spathilingue in Bali Botanical Garden, Indonesia

Cited by 7 publications

References 8 publications

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27

Extracellular enzymes producing yeasts study: cost-effective production of α-amylase by a newly isolated thermophilic yeast <i>Geotrichum candidum</i> PO27

Isolation and Molecular Screening of Fungus as Agents in Cellulolytic Transformation Materials from Symbiotic Lichen

Isolasi dan Karakterisasi Jamur Selulolitik pada Fermetodege: Pakan Fermentasi Berbahan Campuran Eceng Gondok, Bekatul Padi, dan Tongkol Jagung

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