Screening of anti-mycobacterial compounds in a naturally infected zebrafish embryo model

Abstract

20Overall this study has demonstrated that zebrafish embryos naturally infected with bioluminescent 36 M. marinum M can be used for the rapid screening of anti-mycobacterial compounds with readily 37 available equipment and limited expertise. The result is an assay that can be carried out by a wide 38 variety of laboratories for minimal cost and without high levels of zebrafish expertise. 39 40

Aryl Hydrocarbon Receptor Modulation by Tuberculosis Drugs Impairs Host Defense and Treatment Outcomes

“…The zebrafish (Danio rerio) has emerged as valuable animal model to study toxicology (Roper and Tanguay, 2018) and the mechanisms of disease, including TB (Van Leeuwen et al, 2015). Increasingly, zebrafish have been harnessed for highthroughput in vivo screenings of novel drug candidates, such as antimicrobials (Zhong and Lin, 2011;Dalton et al, 2017). We used zebrafish to validate our in vitro findings.…”