Screening of anti‐platelet aggregation agents from <i>Panax notoginseng </i>using human platelet extraction and HPLC–DAD–ESI‐MS/MS

Wang, Jing; Zhao-gang, Huang; Cao, Hui; Wang, Yitao; Peng, Hui; Hoo, Chai; Li, Shao‐Ping

doi:10.1002/jssc.200700507

Cited by 47 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, four typical compounds with antiplatelet aggregation activity, including 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol, were identified. Wang et al (2008) investigated the antiplatelet aggregation candidates from Panax notoginseng (Burkill) F.H. Chen (Araliaceae) (Sanqi in Chinese) using the platelet bio-extraction method.…”

Section: Research Articlementioning

confidence: 99%

“…As reported previously (Wang et al 2008), the TAE (7.5 mg/mL, 130 lL) and the WP suspension (3.9 mL) were incubated at 37 C for 30 min with shaking. After incubation, the mixture was centrifuged at 2259g for 10 min with a Centriprep V R 50 K device (Merk Millipore Ltd., Tullagreen, Carrigtwohill, Co. Cork, Ireland) to obtain filtrate, and then the suspension was subjected to HPLC analysis.…”

Section: Platelet Bio-specific Extractionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis

Zhang

Chen

Wang

et al. 2016

Pharmaceutical Biology

View full text Add to dashboard Cite

Objective: The antiplatelet aggregation compounds in Rhizoma Corydalis were screened to validate its traditional medicinal use. Material and methods: Total alkaloid extract (TAE) of Rhizoma Corydalis was obtained by refluxing 100 g Rhizoma Corydalis powder with 600 mL 70% ethanol, and purified by acidification (20% HCl) and alkalization (5 M NaOH) process. Potential antiplatelet aggregation compounds in TAE were screened by a method involving platelet bio-specific extraction and HPLC-DAD/LC-MS analysis. Further in vitro antiplatelet aggregation activity confirmation of TAE and seven main alkaloids were achieved by turbidimetry method within 3 h after blood collection from rabbit carotid artery, and all the test drugs were at the concentration range of 25-350 lg/mL. Finally, HPLC-DAD was employed for the quantitative determination of seven main components in TAE. Results: Five alkaloids, identified as glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline, can be specifically extracted with platelets. The results indicated that all these five alkaloids can inhibit thrombin-induced platelet aggregation in a low dose (IC 50 of glaucine, dehydrocorydaline, canadine, tetrahydrocoptisine and corydaline were 49.057, 34.914, 33.547, 84.261 and 54.164 lg/mL, respectively) as compared to TAE (IC 50 ¼ 175.426 lg/mL) and aspirin (IC 50 ¼ 300.340 lg/mL), while the unbound compounds (palmatine and tetrahydropalmatine) had a very weak antiplatelet effect (IC 50 > 200 lg/mL). Discussion and conclusion: This study is the first reported work for antiplatelet components screening in Rhizoma Corydalis. Seven compounds were detected and identified by HPLC-DAD/LC-MS, of which five platelet-targeted compounds were discovered. ARTICLE HISTORY

show abstract

Section: Research Articlementioning

confidence: 99%

Section: Platelet Bio-specific Extractionmentioning

confidence: 99%

Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis

Zhang

Chen

Wang

et al. 2016

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…In vitro study (52,53,59) Animal study (54,57,58) -CYP3A4, 2C9, 2C19, 2D6 inhibitor (52)(53)(54) -Antiplatelet effect (57)(58)(59) -Anticoagulant effect (18,58) Case report (25,26) Clinical study (27) Lycium Lycium barbarum Dried ripe fruit: 枸杞子 Case report (28,29) ----Ginger Zingiber officinale Fresh: 生姜, Dried: 干姜 Clinical study (30,63,64) In vitro study (62) -Antiplatelet effect (62,64) -Fibrinolytic effect (63) --Clinical study (15) Notoginseng Panax notoginseng Root: 三七 Animal study (32,65,66) -Increases half-life of warfarin (32) -Antiplatelet effect (65,66) ---HSA: human serum albumin…”

Section: Peach Kernelmentioning

confidence: 99%

Interaction between warfarin and Chinese herbal medicines

Chua¹,

Ang²,

Zhong³

et al. 2015

smedj

View full text Add to dashboard Cite

In traditional Chinese medicine (TCM), the human body is divided into Yin and Yang. Diseases occur when the Yin and Yang balance is disrupted. Different herbs are used to restore this balance, achieving the goal of treatment. However, inherent difficulties in designing experimental trials have left much of TCM yet to be substantiated by science. Despite that, TCM not only remains a popular form of medical treatment among the Chinese, but is also gaining popularity in the West. This phenomenon has brought along with it increasing reports on herb-drug interactions, beckoning the attention of Western physicians, who will find it increasingly difficult to ignore the impact of TCM on Western therapies. This paper aims to facilitate the education of Western physicians on common Chinese herbs and raise awareness about potential interactions between these herbs and warfarin, a drug that is especially susceptible to herb-drug interactions due to its narrow therapeutic range.

show abstract

“…Recently, nucleobases and nucleosides have been proven as important bioactive compounds involved in multiple biological activities such as anti-platelet aggregation, 5,6 anti-arrhythmic 7 and anti-seizure e®ects. 8 A research 9 on the identi¯cation and quanti¯cation of nucleosides and nucleobases in leeches and earthworms was carried out.…”

Section: Introductionmentioning

confidence: 99%

Improvement of NIR models for quality parameters of leech and earthworm medicines using outlier multiple diagnoses

Chen

et al. 2017

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

Leeches and earthworms are the main ingredients of Shuxuetong injection compositions, which are natural biomedicines. Near infrared (NIR) di®use re°ection spectroscopy has been used for quality assurance of Chinese medicines. In the present work, NIR spectroscopy was proposed as a rapid and nondestructive technique to assess the moisture content (MC), soluble solid content (SSC) and hypoxanthine content (HXC) of leeches and earthworms. This study goal was to improve NIR models for accurate quality control of leech and earthworm using outlier multiple diagnoses (OMD). OMD was composed of four outlier detection methods: spectrum outlier diagnostic (MD), leverage diagnostic (LD), principal component scores diagnostic (PCSD) and factor loading diagnostic (FLD). Conventional outlier diagnoses (MD, LD) and OMD were compared, and the best NIR models were those based on OMD. The correlation coe±cients (R) for leech were 0.9779, 0.9616 and 0.9406 for MC, SSC and HXC, respectively. The values of relative standard error of prediction (RSEP) for leech were 2.3%, 5.1% and 9.0% for MC, SSC and HXC, respectively. The values of R for earthworm were 0.9478, 0.9991 and 0.9605 for MC, SSC and HXC, respectively. The values of RSEP for earthworm were 8.8%, 2.4% and 12% for MC, SSC and HXC, respectively. The performance of the NIR models was certainly improved by OMD.

show abstract

Screening of anti‐platelet aggregation agents from Panax notoginseng using human platelet extraction and HPLC–DAD–ESI‐MS/MS

Cited by 47 publications

References 19 publications

Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis

Simultaneous screening and analysis of antiplatelet aggregation active alkaloids from Rhizoma Corydalis

Interaction between warfarin and Chinese herbal medicines

Improvement of NIR models for quality parameters of leech and earthworm medicines using outlier multiple diagnoses

Contact Info

Product

Resources

About