Screening of antidepressant activity and estimation of quercetin from<i>Coccinia indica</i>using TLC densitometry

Randhawa, Kudrat; Kumar, Deepak; Jamwal, Anupam; Kumar, Suresh

doi:10.3109/13880209.2015.1025289

Cited by 25 publications

(17 citation statements)

References 25 publications

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“…The average AUC of the peak corresponding to marker compound was noted at 278 nm in the test sample and its concentration was calculated from the standard plot. The developed TLC densitometric method was validated for the parameters such as linearity, range, limit of detection, limit of quantification, inter-day precision, intra-day precision, accuracy, repeatability and specificity as per ICH guidelines [9] .…”

Section: Tlc Densitometric Methods Development Studiesmentioning

confidence: 99%

“…In the light of adverse effects associated with these synthetic drugs, researchers worldwide are searching for newer, safer and more efficacious alternatives [7,8] . Herbs prove to be useful, safe and potent therapeutic agents to treat various CNS disorders including depression [9] . A wide variety of plants exist in nature popularly reputed to have medicinal properties.…”

Section: Research Papermentioning

confidence: 99%

“…The test drugs were administered orally with the help of an oral cannula fitted on a tuberculin syringe. The antidepressant and locomotor activity studies were investigated using well established models such as forced swim test (FST) and open field test, respectively, as per standard procedures [9] . Distilled water+Tween 80 (2%) was used as a vehicle for preparing various test doses of crude extracts, fractions and the standard drug.…”

Section: Antidepressant Activitymentioning

confidence: 99%

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Screening of Antidepressant Activity and Marker-based Standardization of Baptisia tinctoria (L.) R. Vent.

Kumar¹,

Kumar²

2017

pharmaceutical-sciences

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Richa and Kumar: Antidepressant Activity and TLC Densitometry of Baptisia tinctoriaBaptisia tinctoria (Wild indigo; Fabaceae) has long tradition of use in Indian systems of medicine for the treatment of depression. This investigation deals with the evaluation of the antidepressant activity of various extracts and fractions of B. tinctoria roots and estimation of the content of bioactive principle using thin-layer chromatography densitometry. Roots of B. tinctoria were successively extracted to obtain n-hexane, chloroform, methanol and water extracts. The chloroform, methanol and water extracts were screened for antidepressant activity at doses of 200 or 400 mg/kg, p.o., in mice subjected to forced swim test. Methanol extract showed significant activity at a dose of 400 mg/kg. The methanol extract was further fractionated successively to obtain ethyl acetate and 1-butanol fractions. Antidepressant activity of these fractions was assessed using forced swim test at the doses of 90 and 80 mg/kg, p.o., respectively. The ethyl acetate fraction showed significant antidepressant activity at the dose of 90 mg/kg in mice. Further, specific antidepressant activity without psychostimulant effects of bioactive methanol extract and ethyl acetate fraction was confirmed through evaluation of locomotor behaviour in mice using open field test. Phytochemical screening showed the presence of flavonoids as major class of phytoconstituents in the methanol extract. Comparative thin-layer chromatography fingerprint studies confirmed the presence of hesperitin in methanol extract. Hesperitin was used as a chemical marker to standardize B. tinctoria roots using validated thin-layer chromatography densitometric method and the content of hesperitin was found to be 0.0085% w/w.

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Section: Tlc Densitometric Methods Development Studiesmentioning

confidence: 99%

Section: Research Papermentioning

confidence: 99%

Section: Antidepressant Activitymentioning

confidence: 99%

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Screening of Antidepressant Activity and Marker-based Standardization of Baptisia tinctoria (L.) R. Vent.

Kumar¹,

Kumar²

2017

pharmaceutical-sciences

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“…8 Ayurveda and Unani systems claim C. indica as antidiabetic agent and other traditional uses are anti-inflammatory, antipyretic, analgesic, antimicrobial, antibacterial, antidepressant and expectorant. [9][10][11] Sporadic pharmacological studies have supported traditional claims of C. indica in treating diabetes. But most of these studies have employed uncharacterized crude extract of the plant.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic Activity of Isolated Compound from Coccinia indica

Jamwal¹,

Kumar²

2019

IJPER

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Background and Objective: Coccinia indica Wight. and Arn (Ivy gourd, family Cucurbitaceae) has a long tradition of use in the treatment of various ailments. Ayurveda and Unani systems claim C. indica as antidiabetic agent. Therefore the present investigation was aimed to evaluate antidiabetic activity of isolated compound(s) from C. indica. Materials and Methods: Methanol extract of C. indica aerial parts was prepared by Soxhlet extraction method and ethyl acetate fraction was prepared by partitioning of methanol extract. Ethyl acetate fraction was subjected to antidiabetic activity-guidedfractionation using column chromatography for the isolation of antidiabetic compound(s). Various fractions and isolated compounds were screened for antidiabetic activity using streptozotocin-nicotinamide type-2 diabetic model. The glucose level in rats was determined using glucometer. The antidiabetic biochemical estimations were performed on bioactive isolated compound. Results: Bioactivity-guided-fractionation yielded a bioflavonoid quercetin, which has been isolated for the first time from C. indica aerial parts. Quercetin (5 mg/kg, p.o.) significantly reduced the glucose levels in type-2 diabetic rats but did not produce hypoglycemic effects in oral glucose tolerance test on normal rats. Quercetin significantly increased insulin and serum HDL-cholesterol levels and showed better β-cell functions. Quercetin caused slight change in hepatic marker enzymes and kidney functions markers in diabetic rats. Conclusion: Quercetin is responsible for antidiabetic activity of C. indica aerial parts.

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“…The developed methods were validated for the parameters described in ICH guidelines such as linearity, range, limit of detection (LOD), limit of quantification (LOQ, inter day precision, intra-day precision, accuracy, repeatability and specificity [10] .…”

Section: Abies Pindrowmentioning

confidence: 99%

Quantitative Determination of Shikimic Acid and Pinitol in Abies pindrow Aerial Parts Using TLC

Kumar¹,

Kumar²

2016

ijps

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Kumar and Kumar: TLC Densitometry of Abies pindrow on the Basis of PhenolsAbies pindrow Royle (Himalayan Fir; Pinaceae) has been traditionally used in the treatment of various ailments and reported to contain cyclic polyols as major class of phytoconstituents. But no work has ever been carried out to standardize A. pindrow on the basis of cyclic polyols. Thus, it was considered worthwhile to determine quantitatively contents of main cyclic polyols, i.e., shikimic acid and pinitol in A. pindrow aerial parts using TLC densitometry. Shikimic acid and pinitol were resolved on TLC plates using solvent systems, chloroform:methanol:formic acid (14:6:1) and chloroform:methanol (3:2), respectively. The shikimic acid and pinitol were derivatized with anisaldehyde-sulphuric acid reagent and ammonical silver nitrate reagent respectively. The spots of shikimic acid and pinitol were visualized by heating derivatized plates. The plates were then scanned in TLC scanner at 500 nm. The contents of shikimic acid and pinitol were estimated to be 0.0511% and 0.0987% w/w, respectively, in A. pindrow aerial parts. The developed TLC densitometric methods for estimation of cyclic polyols in A. pindrow aerial parts were found to be precise, accurate and specific on the basis of validation parameters.Key word: Abies pindrow, Pinitol, Shikimic acid, TLC densitometry.Abies pindrow Royle (Silver Fir; family: Pinaceae) is an Indian traditional plant, which is distributed throughout Western Himalayas [1] . The plant has long history of use as anxiolytic, analgesic, antidiabetic and antiinflammatory [2] . The traditional uses of A. pindrow have been validated by scientific studies, but these studies employed crude uncharacterized extracts [3][4][5] . Sporadic phytochemical work has been carried out on the plant. Chalcone glycosides, flavonoids, fatty acids, hydrocarbons and terpenoids have been isolated from A. pindrow [6][7][8][9] . As the case with most plant drugs, A. pindrow has not been standardized on the basis of marker compounds. Marker-based standardization is important for reproducibility of therapeutic efficacy and safety of the plant. High Performance Thin Layer Chromatography (HPTLC) has gained popularity in standardizing plants by estimating the content of, preferably, bioactive constituents. Thus, the present investigation was undertaken to estimate the contents of two main cyclic polyols constituents of A. pindrow using TLC densitometry. Methanol, chloroform (E-Merck, Delhi, India), and formic acid (Ranbaxy Laboratory Chemicals, Mumbai, India), of AR grade, sulphuric acid, glacial acetic acid (E-Merck Ltd., Mumbai), sodium hydroxide, silver nitrate, ammonia solution and anisaldehyde (S.D. Fine Chemicals, Mumbai) were used in present investigations. Shikimic acid and pinitol, isolated from A. pindrow aerial parts, were used as marker compounds. The isolated compounds have been characterized by 1 HNMR and 13 CNMR spectral analysis.The stock solutions of marker compounds were prepared by separately dissolving accurately weighed 5 m...

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Screening of antidepressant activity and estimation of quercetin fromCoccinia indicausing TLC densitometry

Cited by 25 publications

References 25 publications

Screening of Antidepressant Activity and Marker-based Standardization of Baptisia tinctoria (L.) R. Vent.

Screening of Antidepressant Activity and Marker-based Standardization of Baptisia tinctoria (L.) R. Vent.

Antidiabetic Activity of Isolated Compound from Coccinia indica

Quantitative Determination of Shikimic Acid and Pinitol in Abies pindrow Aerial Parts Using TLC

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