Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus

López-Abarrategui, Carlos; Alba, Annia; Lima, Loiane Alves de; Maria-Neto, Simone; Vasconcelos, Ilka M.; Oliveira, José T.A.; Dias, Simoni Campos; Otero‐González, Anselmo J.; Franco, Octávio L.

doi:10.1007/s00284-012-0096-5

Cited by 14 publications

(12 citation statements)

References 28 publications

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“…There are a few reports on potent antimicrobial activities of extracts from gastropods [7,16,[21][22][23]. Rajaganapathi et al (2002) [7] reported the antibacterial activity of gastropods against S. typhi.…”

Section: Discussionmentioning

confidence: 99%

“…Soft bodies of the snails were homogenized separately in a solution containing 10% ice cold acetic acid 1:3 w/v in a blender (1 gm snail tissue and 3 mL of 10% acetic acid). The homogenate was centrifuged at 10,000× g for 30 min at 4 • C. Soluble proteins were precipitated with ammonium sulfate (NH 4 ) 2 SO 4 at 100% saturation with constant stirring for 1 h at 4 • C. After centrifugation at 10,000× g for 30 min at 4 • C, the precipitate was suspended in 10 mM Tris-HCl buffer, pH 7.0 and was further desalted using dialysis membrane [16]. The extracted proteins were stored at 4 • C. The extracted protein concentrations were estimated using the Bradford method [17], with bovine serum albumin (BSA) as a standard.…”

Section: Extraction Of Proteins From Seven Snail Speciesmentioning

confidence: 99%

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Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Ulagesan

Kim

2018

Applied Sciences

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Snails have been used both as a food and as a treatment for a variety of medicinal conditions. In this study, seven different snail proteins were evaluated for their antimicrobial activity. Fresh water and land snails of seven different live species were collected and identified. Crude proteins were extracted from seven different snails. The extracted proteins were estimated using Bradford's method and snail proteins were displayed using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The seven different snail proteins were evaluated for their antimicrobial activity against various pathogenic bacterial and fungal cultures by agar well diffusion method and MIC (Minimum Inhibitory Concentration). One of the most active, crude proteins was from land snail Cryptozona bistrialis and its protein was capable of completely inhibiting the development of pathogenic bacterial and fungal cultures. This study shows that the land snail C. bistrialis proteins could be used as an antibiotic in biomedical research.

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Section: Discussionmentioning

confidence: 99%

Section: Extraction Of Proteins From Seven Snail Speciesmentioning

confidence: 99%

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Ulagesan

Kim

2018

Applied Sciences

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“…Besides palytoxin, not many studies on the biological activity of zoanthid venoms or toxins have been characterized to date. An extract of their soft tissues was tested for antibacterial activity and it was found that it inhibits Escherichia coli and Staphylococcus aureus in 97.7 and 100%, respectively [ 20 ]. More recently, P. caribaeorum extracts were found to have antioxidant effects and cytotoxic activities [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Hemolytic, anticancer and antigiardial activity of Palythoa caribaeorum venom

Lazcano-Pérez

Zavala-Moreno

Rufino-González

et al. 2018

J Venom Anim Toxins Incl Trop Dis

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BackgroundCnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of Palythoa caribaeorum venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite Giardia intestinalis.MethodsThe presence of cytolysins and vasoconstrictor activity of P. caribaeorum venom were determined by hemolysis, PLA2 and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma), MCF-7 (human mammary adenocarcinoma), K562 (human chronic myelogenous leukemia), U251 (human glyoblastoma), PC-3 (human prostatic adenocarcinoma) and SKLU-1 (human lung adenocarcinoma). An in vivo toxicity assay was performed with crickets and the antiparasitic assay was performed against G. intestinalis at 24 h of incubation.ResultsP. caribaeorum venom produced hemolytic and PLA2 activity and showed specific cytotoxicity against U251 and SKLU-1 cell lines, with approximately 50% growing inhibition. The venom was toxic to insects and showed activity against G. intestinalis in a dose-dependent manner by possibly altering its membrane osmotic equilibrium.ConclusionThese results suggest that P. caribaeorum venom contains compounds with potential therapeutic value against microorganisms and cancer.

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“…The promising antimicrobial compounds are antimicrobial peptides (AMPs) which are small sequences comprising amino acids ranging from 10-100 residues that eminently kill the pathogens and could be permanent solution for infections of bacterial origin especially. Though the pathogens develop resistance against each of the existing antibiotics, the specific bactericidal properties of the AMPs will elude the resistance property and end up infection without any side-effects (1) . Novel peptides from various marine sources like sponges, ascidians, sea anemones, seaweeds, etc., are isolated and they are submitted to clinical trials.…”

Section: Introductionmentioning

confidence: 99%

Identification and Isolation of Glycine, Alanine and Asparagine Rich Secretory Protein With Antibacterial Efficiency From Donax Cuneatus

Bibiana¹,

Palanisamy²,

Subbiah³

et al. 2016

Int. Res. J. Pharm.

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The crude protein was extracted from marine edible bivalve Donax cuneatus, precipitated and further dialysed against PBS in dialysis membrane having MW cut off of 3 KDa. The extract was evaluated for its antimicrobial activity against highly pathogenic Gram positive and Gram negative bacteria. It was found to be active against Pseudomonas aeruginosa and Staphylococcus aureus. The purified fractions obtained from anion-exchange column were evaluated for their activity against the aforesaid bacteria. The highly active fraction against Staphylococus aureus with the zone of inhibition of 12 mm was identified and further purified and characterised. The active fraction was enriched with glycine, alanine and asparagine aminoacids.

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Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus

Cited by 14 publications

References 28 publications

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails

Hemolytic, anticancer and antigiardial activity of Palythoa caribaeorum venom

Identification and Isolation of Glycine, Alanine and Asparagine Rich Secretory Protein With Antibacterial Efficiency From Donax Cuneatus

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