Screening of antioxidant and antimicrobial activities on Plectranthus spp. extracts

Rijo, Patrícia; Batista, Mariana Nogueira; Matos, Marisa; Rocha, Helga; Jesus, Sandra; Simões, M. Fátima

doi:10.19277/bbr.9.2.45

Cited by 13 publications

(32 citation statements)

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“…Vários estudos têm vindo a avaliar os extratos de Plectranthus spp. tendo em conta as suas propriedades antioxidantes e futura avaliação fitoquímica de compostos ativos, transformando estas plantas num importante motivo de estudo (3) . Os compostos antimicrobianos de origem natural geralmente apresentam estruturas químicas complexas que podem ser importantes nas interações específicas e reconhecimento de alvos macromoleculares potenciais, nos microrganismos patogénicos.…”

Section: Methodsunclassified

“…Regarding the antioxidant properties of the Lamiaceae family and future phytochemical evaluation of active compounds, there are several related reports of Plectranthus spp. extracts that suggest these plants as an important study motif (3) . The antimicrobials of natural origin usually present complex chemical structures, which may be important for specific interactions and recognition of potential macromolecular targets in the pathogenic microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…The scientific validation on the traditional medicine of these plants is an important issue, to search for pharmacological activities and through drug development. In fact, there has been an increasing interest on the Plectranthus genus for the investigation of its therapeutic values (3,4) . Regarding this, the herein described study evaluated several biological activities of seven Plectranthus species (P. swynnertonii S. Moore, P. welwischii Briq.…”

Section: Introductionmentioning

confidence: 99%

“…A procura de atividades farmacológicas até o desenvolvimento de fármacos, através da validação científica da medicina tradicional destas plantas é um assunto importante. Na verdade, tem havido um aumento na pesquisa de interesses terapêuticos do género Plectranthus (3,4) . Assim, no presente estudo são descritas várias atividades bioló-gicas de sete espécies de Plectranthus (P. swynnertonii S. Moore, P. welwischii Briq.…”

Section: Introductionunclassified

“…Indeed, it is known that both the metabolites and extracts of several Plectranthus spp. have exhibited activity against Gram-positive and Gram negative bacteria and yeasts (12)(13)(14) . Brine shrimp lethality assay is a technique commonly used to investigate the toxic potential of plant extracts (15) .…”

Section: Introductionmentioning

confidence: 99%

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Biological activity screening of seven Plectranthus species

Ntungwe

Marçalo²,

García³

et al. 2017

BBR

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Biopharmaceutical Sciences│Ciências BiofarmacêuticasBiomed Biopharm Res. , 2017; (14) 1: , 95-108 95 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Biological activity screening of seven Plectranthus species AbstractNatural products from Plectranthus spp. plants have an ethnopharmacological use, inspiring several scientific investigations. As such, this work aims to perform a biological activity screening in order to scientifically validate the use of these plants. Assays on in vitro acetylcholinesterase (AChE) inhibition, antioxidant effects, antimicrobial activity and Artemia salina lethality were performed on seven Plectranthus spp. extracts (P. swynnertonii, P. welwischii, P. woodii, P. cylindraceus, P. spicatus, P. ramosior and P. petiolaris). Acetonic extracts were obtained by sonication (10% w/v), where P. ramosior had the highest yield of dry extract (13.49% w/w). In the AChE inhibition assay, only P. cylindraceus extract decreased enzymatic activity (30.2 ± 3.78%). The antimicrobial activity was screened using the well diffusion method, against Gram positive and negative bacteria and yeast. P. ramosior extract showed not only an inhibition zone against S. aureus and C. albicans (15 and 11 mm, respectively), but also the highest scavenging activity (DPPH method, 36.4 ± 0.04%). On the lethality test in A. salina, P. swynnertonnii extract was the most toxic (LC50 = 0.036 mg/L). These preliminary results showed that P. cylindraceus, P. ramosior and P. swynnertonnii are potential bioactive extracts for further isolation and antimicrobial and cytotoxic studies.Keywords: Plectranthus, Acetylcholinesterase, Antioxidant, Antimicrobial, Toxicity ResumoOs produtos naturais das espécies de plantas Plectranthus têm um uso etnofarmacológico, suscitando vários estudos científicos. Neste trabalho pretendeu-se validar cientificamente estas plantas, realizando uma pesquisa de atividades biológicas. Extratos de sete espécies de Plectranthus (P. swynnertonii, P. welwischii, P. woodii, P. cylindraceus, P. spicatus, P. ramosior e P. petiolaris) foram testados na inibição da acetilcolinesterase in vitro (AChE), atividades antioxidante e antimicrobiana, e toxicidade em Artemia salina. Os extratos acetónicos foram obtidos por ultrassons (10% m/v), dos quais P. ramosior obteve o rendimento de extração mais elevado (13,49% m/m). Todos os extratos foram testados, no entanto apenas o P. cylindraceus obteve inibição da atividade da AChE (30,2 ± 3,78%). A atividade antimicrobiana foi avaliada usando o método de difusão em poços, contra bactérias Gram-positivas, -negativas e leveduras. P. ramosior revelou não só inibição contra S. aureus e C. albicans (15 e 11 mm, respetivamente), como também a mais elevada atividade antioxidante (método DPPH, 36,4 ± 0,04%). No ensaio em A. salina P. swynnertonnii foi o extrato mais tóxico (LC50 = 0,036 mg/L). Os resultados preliminares obtidos neste trabalho, revelam a potencial biotividade dos extratos de P. cyl...

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Section: Methodsunclassified

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

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Biological activity screening of seven Plectranthus species

Ntungwe

Marçalo²,

García³

et al. 2017

BBR

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Extraction Optimization and Structural and Thermal Characterization of the Antimicrobial Abietane 7α-Acetoxy-6β-hydroxyroyleanone

et al. 2018

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The abietane 7α-acetoxy-6β-hydroxyroyleanone (AHR), obtained from plant extracts, is an attractive lead for drug development, given its known antimicrobial properties. Two basic requirements to establish any compound as a new drug are the development of a convenient extraction process and the characterization of its structural and thermal properties. In this work seven different methods were tested to optimize the extraction of AHR from Plectranthus grandidentatus. Supercritical fluid extraction (SFE) proved to be the method of choice, delivering an amount of AHR (57.351 μg·mg) approximately six times higher than the second best method (maceration in acetone; 9.77 μg·mg). Single crystal X-ray diffraction analysis of the ARH molecular and crystal structure carried out at 167 ± 2 K and 296 ± 2 K showed only a single phase, here dubbed form III (orthorhombic space group P222), at those temperatures. The presence of two other polymorphs above room temperature was, however, evidenced by differential scanning calorimetry (DSC). The three forms are enantiotropically related, with the form III → form II and form II → form I transitions occurring at 333.5 ± 1.6 K and 352.0 ± 1.6 K, respectively. The fact that the transitions are reversible suggests that polymorphism is not likely to be an issue in the development pharmaceutical formulations based on ARH. DSC experiments also showed that the compound decomposes on melting at 500.8 ± 0.8 K. Melting should therefore be avoided if, for example, strategies to improve solubility based on the production of glassy materials or solid dispersions are considered.

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Screening the dermatological potential of Plectranthus species components – antioxidant and inhibitory capacities over elastase, collagenase and tyrosinase

Marçalo

García

Nicolai

et al. 2017

65th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2017)

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A series of Plectranthus spp. plant extracts (aqueous, acetonic, methanolic and ethyl acetic) obtained from eight different species, and previously isolated compounds (ranging from polyphenols, diterpenes and triterpenes), were assayed for in vitro inhibition of the skin-related enzymes tyrosinase, collagenase and elastase, and for studying their antioxidant properties. The ethyl acetic extracts of P. grandidentatus and P. ecklonii registered the highest antioxidant activity, whereas acetonic, methanolic and ethyl acetic extracts of P. ecklonii, P. grandidentatus, P. madagascariensis and P. saccatus concerning the enzymatic inhibition assays revealed high anti-tyrosinase and anti-collagenase activities. From the isolated compounds tested, abietane diterpenes and triterpenes were highly active against tyrosinase and elastase activity. Overall, the experimental results showed the powerful antioxidant and inhibitory action on skin-related enzymes tyrosinase, collagenase and elastase of Plectranthus spp. extracts and/or isolated compounds, supporting their further research as bioactive metabolites against skin sagging and hyperpigmentation in cosmetic and pharmaceutical formulations.

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Screening of antioxidant and antimicrobial activities on Plectranthus spp. extracts

Cited by 13 publications

References 13 publications

Biological activity screening of seven Plectranthus species

Biological activity screening of seven Plectranthus species

Extraction Optimization and Structural and Thermal Characterization of the Antimicrobial Abietane 7α-Acetoxy-6β-hydroxyroyleanone

Screening the dermatological potential of Plectranthus species components – antioxidant and inhibitory capacities over elastase, collagenase and tyrosinase

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