Screening of cardioprotective diseases bioactive components from Danshen extracts and LC–MS analysis

Abstract

A rapid and efficient analysis and screening method is adopted for cell affinity capture coupled with HPLC–MS (CAC–HPLC–MS) analysis of bioactive components that have possible efficiency against cardiovascular diseases. This method involves affinity capture, concentration, and separation of bioactive components from Danshen library using oxidatively damaged endothelial cells induced by H2O2, as well as analysis and identification of targeted compounds with HPLC and MS. It combines the specific interaction between cell membrane receptors and bioactive components with the powerful analysis and identification function of HPLC–MS. The CAC–HPLC–MS method was also used for analysis and screening of bioactive components from crude extracts of Danshen. A total of 19 components were found to be bound to oxidatively damaged endothelial cells with seven of these identified. Existing literature confirms that these seven components have many activities related to cardioprotective diseases. Therefore, the combination of biological affinity capture with HPLC–MS should be regarded as an attractive method with great potential for rapid and efficient screening of bioactive components related to anti‐cardiovascular diseases from natural product libraries.

<sup>1</sup>H NMR Metabolic Fingerprints of Cabernet Sauvignon Grapes Produced in the Jinsha River and Lancan River Valleys in the Shangeri-La Region, South China

“…However, Jinsha River valley compared to the Lancan River valley had decreased arginine and proline metabolism. The OPL-DA model showed an enhanced caffeine pathway in the Jinsha River valley via xanthine alkaloid compounds, which have multiple functions in biologic processes, including antioxidant activity, and can alter the cellular redox and inflammatory status in a dose-dependent manner [21].…”

Danshen-Shanzha formula for the treatment of atherosclerosis: ethnopharmacological relevance, preparation methods, chemical constituents, pharmacokinetic properties, and pharmacological effects

“…was proven to have many active ingredients, including diterpenoids, triterpenoids, lactone, nitrogenous, phenolic acids, and other compounds ( Jia et al, 2019 ). According to the Chemistry Database, over than a hundred compounds have been identified from DS until so far, including water-soluble phenylpropionic acids such as salvianolic acid A/B/C/D/E/F/G, lipid-soluble phenanthraquinones such as tanshinone I/IIA/IIB/V/VI, dihydrotanshinone І, tanshindiol A, miltirone, dehydromiltirone and isotanshinone, etc ( Su et al, 2015 ; Yuan et al, 2020 ). Readers who are interested in obtaining further information regarding the chemical structures of DS compounds are advised to refer to the specialized review of Salvia miltiorrhiza Bge conducted by Wang et al ( Wang et al, 2017 ).…”

Comparative Efficacy of Danshen Class Injections for Treating Acute Coronary Syndrome: A Multidimensional Bayesian Network Meta-Analysis of Randomized Controlled Trials

“…In China and Japan, Danshen ( Radix Salviae Miltiorrhizae ) is the dried root or rhizome of Salvia miltiorrhiza Bunge , commonly used to treat cardiovascular-related diseases ( Meim et al, 2019 ; Yuan et al, 2020 ). The relative studies validated that Danshen possesses the characteristics of relaxing coronary artery muscle, improving microcirculation, reducing blood viscosity and inhibiting thrombogenesis ( Cheng, 2006 ).…”