Screening of cashmere fineness-related genes and their ceRNA network construction in cashmere goats

Hui, Taiyu; Zheng, Yuanyuan; Chang, Yue; Wang, Yanru; Bai, Zhixian; Sun, Jiaming; Cai, Weidong; Zhang, Xinjiang; Bai, Wenlin; Wang, Zeying

doi:10.1038/s41598-021-01203-8

Cited by 19 publications

(7 citation statements)

References 66 publications

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“…Genome‐wide association study (GWAS) of morphological traits in Sudanese goats, showed that CNTNAP5 gene was associated with diameter of bicoastal (Rahmatalla et al, 2018). One of the significant genes in the current study, ITGB1, was identified in Hui et al's study to regulate bladder cancer in goats (Hui et al, 2021). Also, in another study on goat mammary epithelial cells, this gene led to G1 phase blocking and pathway activation in GMECs by promoting S phase (Zhang et al, 2020).…”

Section: Discussionmentioning

confidence: 85%

Identification of selection signatures in Capra hircus and Capra aegagrus in Iran

Taheri

Saedi

Zerehdaran

et al. 2023

Animal Science Journal

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Identification of selection signatures may provide a better understanding of domestication process and candidate genes contributing to this process. In this study, two populations of domestic and wild goats from Iran were analyzed to identify selection signatures. RSB, iHS, and XP‐EHH statistics were used in order to identify robust selection signatures in the goat genome. Genotype data of domestic and wild goats from the NextGen project was used. The data was related to 18 Capra aegagrus (wild goat) and 20 Capra hircus (domestic goat) from Iran. The iHS method indicated 675 and 441 selection signatures in C. aegagrus and C. hircus, respectively. RSB and XP‐EHH methods showed about 370 and 447 selection signatures in C. aegagrus and C. hircus, respectively. These selection signatures were mainly associated with milk production, fleece trait, mammary epithelial cells, reproduction, and immune system.

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Section: Discussionmentioning

confidence: 85%

Identification of selection signatures in Capra hircus and Capra aegagrus in Iran

Taheri

Saedi

Zerehdaran

et al. 2023

Animal Science Journal

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“…Over the past few years, lncRNAs have emerged as a previously undiscovered regulator of gene expression that is capable of regulating a variety of cellular processes. Related studies have reported that lncRNAs LNC_000972, LNC_000503 and LNC_000881 may regulate hair follicle cycle through WNT3A, HOXC13 and MSX235 (Hui et al, 2021). Accumulating evidence has indicated that the effect of lncRNA on mRNA may regulate its function.…”

Section: Discussionmentioning

confidence: 99%

Screening and bioinformatics analysis of a potential ceRNA network in melatonin-induced cashmere growth in Liaoning cashmere goats

Jin,

Fan,

Lyu

et al. 2024

Arch. Anim. Breed.

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Abstract. The purpose of this study was to investigate the effect of melatonin (MT) on the expression patterns of lncRNA, mRNA and miRNA in Liaoning cashmere goat (LCG) skin fibroblasts. A quantity of 200 ng L−1 MT (MT group) stimulated LCG skin fibroblasts for 48 h, and RNA sequencing was conducted with the control group (Con group) (n=3). The ceRNA network was constructed by bioinformatics analysis of the sequencing data and transmission electron microscopy observation of coated pits and endocytic vesicles. In this study, the results indicated that MT treatment significantly facilitated the proliferation of LCG skin fibroblasts and increased the number of coated pits and vesicles. A total of 775 mRNAs, 57 lncRNAs and 10 miRNAs had differential expression, as indicated by RNA sequencing of skin fibroblasts administrated on the MT group and Con group. The regulatory network of ceRNA was studied, and the results suggested that inositol phosphate metabolism, the cGMP–PKG signaling pathway, endocytosis and other pathways played a certain role in the growth and development of the LCG cashmere. Moreover, the key genes (e.g., CREB1, PIK3C3, AGAP3, MEF2A, ASAP2, IRAG1, PNISR, PIP5K1A, SRSF11, ZRANB2, RBM39 and CBL) were regulated by chi-miR-34c-5p, chi-miR-34c-3p and chi-miR-195-5p. The above mRNAs were competitively bound by 15 lncRNAs (e.g., MSTRG.28630.12, MSTRG.28660.14, MSTRG.28099.7). And through dual luciferase and other experiments, it was further confirmed that PIP5K1A is the target gene of miR-34c-5p. This finding provides new insights into the molecular mechanism by which melatonin promotes villi growth in cashmere.

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“…Based on the above precise screening and prediction results, a ceRNA regulatory network for secondary hair follicle development was constructed consisting of 13 circRNAs, 21 miRNAs, and 110 mRNAs. Hui constructed a ceRNA regulatory network related to the fineness of cashmere ( 43 ), Liu constructed a ceRNA regulatory network of dairy goat endometrial epithelial cells ( 44 ), and Yue constructed a ceRNA regulatory network related to skeletal muscle development in Qinchuan cattle ( 45 ). All of them provide an important basis for exploring the functional role of circRNA/lncRNA through the ceRNA mechanism.…”

Section: Discussionmentioning

confidence: 99%

Construction and functional analysis of ceRNA regulatory network related to the development of secondary hair follicles in Inner Mongolia cashmere goats

Shang

Rong

et al. 2022

Front. Vet. Sci.

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Cashmere goat hair follicles are divided into primary hair follicles and secondary hair follicles. The primary hair follicles produce coarse hair, and the secondary hair follicles produce cashmere. The development of hair follicles is affected by a variety of signaling molecules and pathways. Studies have shown that non-coding RNAs are widely involved in the development of hair follicles of the goat, including small RNAs (miRNAs), long non-coding RNAs (lncRNA), and circular RNAs (circRNAs). In recent years, circRNAs, as a new type of circular closed non-coding RNAs, have attracted great attention due to their high stability. However, its regulatory effect on cashmere goat hair follicles mainly focuses on the periodic regulation of secondary hair follicles, and there is no report on the development of cashmere goat hair follicles during the fetal period. Therefore, this study was based on the circRNA, miRNA, and mRNA expression profiles obtained by whole-transcriptional sequencing of the skin tissue of the Inner Mongolia cashmere goats in the fetal period (days 45, 55, 65, and 75) and screening out the morphological changes of hair follicles at different periods. A total of 113 circRNAs related to the development of secondary hair follicles were present. According to the principle of the ceRNA regulatory network, a ceRNA regulatory network composed of 13 circRNAs, 21 miRNAs, and 110 mRNAs related to the development of secondary hair follicles was constructed. Then, qRT-PCR and Sanger sequencing identified circRNA2034, circRNA5712, circRNA888, and circRNA9127 were circRNAs. Next, the dual-luciferase reporter gene verified the targeting relationship of circRNA5712-miR-27b-3p-Dll4. In conclusion, this study constructed a ceRNA regulatory network for the development of cashmere goat secondary hair follicles, laying a foundation for the analysis of circRNAs regulating the morphogenesis and development of cashmere goat secondary hair follicles through the ceRNA mechanism.

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Screening of cashmere fineness-related genes and their ceRNA network construction in cashmere goats

Cited by 19 publications

References 66 publications

Identification of selection signatures in Capra hircus and Capra aegagrus in Iran

Identification of selection signatures in Capra hircus and Capra aegagrus in Iran

Screening and bioinformatics analysis of a potential ceRNA network in melatonin-induced cashmere growth in Liaoning cashmere goats

Construction and functional analysis of ceRNA regulatory network related to the development of secondary hair follicles in Inner Mongolia cashmere goats

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