Screening of entomopathogenic Deuteromycetes for activities on targets involved in degenerative diseases of the central nervous system

Schmidt, Karen; Li, Zhaoyu; Schubert, Barbara; Huang, Bo; Stoyanova, Stefka; Hamburger, Matthias

doi:10.1016/j.jep.2003.08.009

Cited by 34 publications

(31 citation statements)

References 25 publications

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“…Enzyme inhibition was measured using the method described by Holt et al 24 and Schmidt et al 25 with minor modifications. Samples were serially diluted with distilled water, and 40 mL of each dilution was placed in 96-well microplates (PS Microplate, non-sterile, Greiner Bio-One, Germany) to give final concentrations from 5 to 0.00005 mg/mL (five dilutions for samples).…”

Section: Measurement Of Inhibitionmentioning

confidence: 99%

Total monoamine oxidase (MAO) inhibition by chestnut honey, pollen and propolis

Yıldız

Karahalil

Can

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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Monoamine oxidase (MAO) inhibitors are generally used in the treatment of depressive disorders and some neurodegenerative illnesses, such as Parkinson's disease and Alzheimer's disease. The aim of this preliminary study was to investigate the MAO [MAO (E.C.1.4.3.4)] inhibiting effect of various apitherapeutic products, such as chestnut honey, pollen and propolis. Extracts' MAO inhibition was measured using peroxidase-linked spectrophotometric assay in enzyme isolated from rat liver microsomes, and the values are expressed as the inhibition concentration (IC 50 ) causing 50% inhibition of MAO. The antioxidant activity of the bee products was also determined in terms of total phenolic content (TPC) and ferric reducing/ antioxidant power in aquatic extracts. All samples exhibited substantial inhibition of MAO, propolis having the highest. Inhibition was related to samples' TPCs and antioxidant capacities. These results show that bee products possess a sedative effect and may be effective in protecting humans against depression and similar diseases.

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Section: Measurement Of Inhibitionmentioning

confidence: 99%

Total monoamine oxidase (MAO) inhibition by chestnut honey, pollen and propolis

Yıldız

Karahalil

Can

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Cordyceps sinensis is a well-known fungal drug used in Traditional Chinese Medicine as a tonic. The pharmacological properties and chemical constituents of Cordyceps sinensis, related Cordyceps species, and their anamorphs have been extensively studied (Chen, Cao, & Huang, 2005;Schmidt et al, 2003;Zhu, Halpern, & Kenneth, 1998). For that natural Cordyceps were becoming less and less recently, they were tried to be replaced with fermentation products of Cordyceps related fungi such as Hirsutella sinensis and Paecilomyces militaris.…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolites in a soybean fermentation broth of Paecilomyces militaris

Huang

et al. 2009

Food Chemistry

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“…Baker also exhibited good MAO-B inhibition activity. MAO activity was detected using a continuous peroxidaselinked photometric assay which was carried out in the 96-well microplate format modified from Holt et al (1997) and Schmidt et al (2003). Positive controls were clorgyline (selective MAO-A inhibitor) and selegiline (R-deprenyl) (selective MAO-B inhibitor) which exhibited IC 50 values of 31 nM and 111 nM, respectively.…”

Section: Studies On Plants Used To Treat Parkinson's Disease In Southmentioning

confidence: 99%