Screening of Hyaluronidase Inhibitor in Korean Medicinal Plants

Hwang, Seon Gu; Yang, Anna; Kim, Soo Jung; Kim, Min Kee; Kim, Sung Soo; Oh, Hyun Jung; Lee, Jung Dae; Lee, Eun Ju; Nam, Kwanghee; Han, Man-Deuk

doi:10.5352/jls.2014.24.5.498

Cited by 8 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Curcuma aromatica exhibited the best hyaluronidase inhibition activity (95%). Large-scale studies were performed by Hwang et al [ 38 ], in which the inhibitory activity of 500 methanolic extracts of 500 species from medicinal plants was analyzed. Only three MeOH extracts inhibited more than 50% of Hyal activity at a concentration of 2 mg/mL.…”

Section: Resultsmentioning

confidence: 99%

EleutherococcusSpecies Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities

Adamczyk

Olech

Abramek

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Secondary metabolites of the roots of Eleutherococcus spp. cultivated in Poland, or the bioactivity, are not fully known. The 75% methanol extracts of five Eleutherococcus spp. (E. senticosus, E. divaricatus, E. sessiliflorus, E. gracilistylus, and E. henryi) were examined for the content of polyphenols and phenolic acids as well as for antiacetylcholinesterase, antihyaluronidase, anti-DPPH∗, and cytotoxic activities. The richest in polyphenols were the roots of E. henryi (10.4 mg/g DW), while in flavonoids the roots of E. divaricatus (6.5 mg/g DW). The richest in phenolic acids occurred the roots of E. henryi [protocatechuic acid (1865 μg/g DE), caffeic acid (244 μg/g DE), and p-coumaric and ferulic acids (55 μg/g DE)]. The highest inhibition of AChE was observed for E. gracilistylus and E. sessiliflorus (32%), at the concentration of 100 μg/0.19 mL of the reaction mixture, while that of Hyal for the roots of E. henryi (40.7%), at the concentration of 100 μg/0.16 mL of the reaction mixture. Among five species tested, the E. henryi extract exhibited the strongest HL-60 cell line growth’s inhibition (IC50 270 μg/mL). The extracts reduced DPPH∗ in a time-dependent mode, at the concentration of 0.8 mg/mL. After 90 min from 14.7 to 26.2%, DPPH∗was reduced. A phytochemical composition and activity of the Eleutherococcus species, cultivated in Poland, are still under research; however, on the basis of the results obtained, it may be concluded that they may become a source of phytochemicals and be useful for Europe’s citizens.

show abstract

Section: Resultsmentioning

confidence: 99%

EleutherococcusSpecies Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities

Adamczyk

Olech

Abramek

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…Hwang et al [8] Kwon et al [12] reported that roots of A. japonica showed relatively high antioxidant activities. In this study, DPPH values of A. japonica were slightly high (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…HAase assay was validated by demonstrating that pure tannic acid (0.07 mg/ml Sigma-Aldrich Co., England) as a positive control, a known HAase inhibitor [7], gives 76-80% enzyme inhibition [8]. All experiments were done in triplicate.…”

Section: Hyaluronidase Inhibition Assaymentioning

confidence: 99%

Evaluation of Achyranthes japonica Ethanol Extraction on the Inhibition Effect of Hyluronidase and Lipoxygenase

Cho¹

2015

Journal of Life Science

View full text Add to dashboard Cite

The 1, 1-diphenyl 2-picrylhyorazyl (DPPH) is a well-known radical and a trap (scavenger) for other radicals. Hyaluronidase (HAase) is an enzyme that depolymerizes the polysaccharide hyaluronic acid (HA) in the extracellular matrix of connective tissue. Lipoxygenase (LOX) enzyme was reported to convert the arachidonic, linoleic and other polyunsaturated fatty acid into biologically active metabolites involved in the inflammatory and immune responses. The purpose of the present study is to evaluate plant extracts as sources of natural antioxidants and to examine whether Achyranthes japonica having significant DPPH, HAase and LOX inhibitory activity. The inhibitory effect of HAase by A. japonica was assayed using a Morgan microplate assay. The antioxidant activity of the A. japonica extracts was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhyorazyl (DPPH) free radical. DPPH scavenging activity of matured roots of A. japonica was evaluated at 4.0 mg/ml was 87.8% and that of young roots was 86.2% at same concentration. The roots of A. japonica showed maximum inhibition of HAase activity (IC50 = 27.7 μg/ml). The highest LOX inhibition was recorded in the root extract among three vegetative parts. Inhibition of HAase activity of roots may contribute towards the development of herbal medicines. Although percent inhibition of lipoxygenase by Achyranthes japonica for all young and matured groups for leaves, stems, and roots at different concentrations, there were not show a statistically significant difference (p<0.05).

show abstract

“…A major screening study on 500 Korean Medicinal plants as HAase inhibitors identified the stem extract of three species possessing relatively higher anti-HAase activity include plant specied Styrax japonica (57.28%), Deutzia coreana (53.50%), and Osmanthus insularis (53.19%). The study further explores that the HAase inhibition could be due to presence of multifunctional compounds and may be effective in preventing allergic reactions and inflammation [81].…”

Section: Anti-hyaluronidase Medicinal Plant Extractsmentioning

confidence: 99%

Plant Natural Products: A Promising Source of Hyaluronidase Enzyme Inhibitors

Bhatti¹,

Karim²

2021

Biochemistry

View full text Add to dashboard Cite

Hyaluronidase enzyme degrades hyaluronan, the primary component of the extracellular matrix found in connective tissues animals and on the surface of certain pathogenic bacteria. The degradation of hyaluronan is linked to a wide range of physiological and pathological process. Inhibiting the hyaluronidase enzyme is thus significant as an approach to treat a variety of diseases and health conditions such as anti-fertility, anti-tumor, antimicrobial, and anti-venom/toxin agents. HAase inhibitors of different chemical types have been identified include both synthetic compounds and constituents obtained from naturally sources. Plant natural products as HAase inhibitors are unique due to their structural features and diversity. Medicinal plants have historically been used as contraceptives, antidote for snakebites and to promote wound healing. In recent years, small molecules, particularly plant natural products (alkaloids, flavonoids, polyphenol and flavonoids, triterpenes and steroids) possessing potent HAase have been discovered. A number of plant species from various families, which have folk medicinal claims for these ailments (related to hyaluronan disturbances) were scientifically proven for their potential to block HAase enzymes.

show abstract

Screening of Hyaluronidase Inhibitor in Korean Medicinal Plants

Cited by 8 publications

References 22 publications

EleutherococcusSpecies Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities

EleutherococcusSpecies Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities

Evaluation of Achyranthes japonica Ethanol Extraction on the Inhibition Effect of Hyluronidase and Lipoxygenase

Plant Natural Products: A Promising Source of Hyaluronidase Enzyme Inhibitors

Contact Info

Product

Resources

About