Screening of immunocompromised patients at risk of strongyloidiasis in western Turkey using ELISA and real-time PCR

Kıvrak, Esra Erdem; Şahar, Esra Atalay; Can, Hüseyin; Döşkaya, Mert; Yılmaz, Mümtaz; Pullukçu, Hüsnü; Caner, Ayşe; Töz, Hüseyin; Gürüz, Adnan Yüksel; Taşbakan, Meltem

doi:10.3906/sag-1605-19

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…Florida has history of Strongyloides infections as published in the MMWR finding sporadic cases among long‐term care facility patients and a 11–50% sero‐positivity 5,6 . Hyperinfection or disseminated disease is a potential life threatening outcome in a child previously exposed to Strongyloides stercoralis and then placed on immunosuppressive drugs 1,7,8 . This unusual but serious complication can most commonly occur in the setting of solid organ transplantation 9 .…”

Section: Introductionmentioning

confidence: 99%

Describing universal Strongyloides serologic screening among pediatric intestinal and liver transplant recipients

Chatani

Selvaggi

García

et al. 2021

Pediatric Transplantation

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Background Strongyloides spp hyperinfections are a worldwide phenomenon that proves fatal for solid organ transplant recipients. Screening protocols to guide prophylaxis management vary institution to institution from universal to epidemiology driven. Our institution initiated a universal screening protocol regardless of travel history and exposure to ensure no cases were missed. Methods In this study, we describe the outcomes of three Strongyloides sero‐positive children whom underwent intestinal or liver transplantation and the experience of universal screening at a tertiary care county hospital in South Florida. Results Among the 66 intestine and liver pediatric transplant recipients who were screened for Strongyloides antibodies, only three were identified to be sero‐positive via the screening mechanism. Two of three had significant epidemiology risk factors. None of the patients reviewed were found to have developed hyperinfection. However, reflecting on the experience represented by our series of pediatric patients, the risk of any complication related to Strongyloides status appears low. Even among this South Florida population whom come from or travel to endemic regions are in contact with sero‐positive individuals, very few illustrate sero‐positivity. Conclusion While institutions continue to analyze the cost‐benefit of universal testing vs. universal prophylaxis vs. targeted screening, the decision must encompass the patient population, rolling cumulative incidence, and morbidity and mortality related to this disease.

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Section: Introductionmentioning

confidence: 99%

Describing universal Strongyloides serologic screening among pediatric intestinal and liver transplant recipients

Chatani

Selvaggi

García

et al. 2021

Pediatric Transplantation

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Strongyloides, cryptosporidium, and other parasitic infections

González¹,

Stillwell²

2021

Pediatric Transplant and Oncology Infectious Diseases

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Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme-Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

et al. 2019

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Introduction Patients on corticosteroids therapy (POCT) are more likely to flare up concealed Strongyloides infection and develop Strongyloides hyperinfection syndrome and/or dissemination. Such critical complications can lead to high mortality rates. Rapid detection methods are, therefore, necessary to detect Strongyloides infection in POCT with the advantage of being applicable in a developing country. Methods Two hundred POCT have been enrolled in this study to determine the rate of infection with Strongyloides. Three different groups of POCT (Strongyloides infected, non-infected, infected with other parasites) were used to evaluate the antibodies detection capability of two serological techniques (enzyme-linked immunosorbent assay (ELISA) and gelatin particles indirect agglutination (GPIAT) against the results of the gold standard agar plate culture (APC). Results With APC, the infection rate of Strongyloides stercoralis in POCT was 9.5% (19/200). POCT with Strongyloides infection displayed related risk factors (job, rural settlements, and soil contact) for infection combined with the subtropical nature of Ismailia Governorate. With regard to serology, ELISA detection results were poor compared to APC with sensitivity and specificity of 42.1% and 82.6%, respectively, and positive and negative predictive values of 72% and 30%. GPIAT appeared to be closely related to APC with sensitivity and specificity of 89.4% and 81.8%, respectively, and positive and negative predictive values of 80.9% and 96.7%. Statistical moderate correlation was detected between GPIAT and ELISA. ConclusionThe GPIAT technique is more convenient, easier, cheaper and faster to rule out the infection of Strongyloides in POCT. It might be the test of choice for routine immunodiagnosis of human strongyloidiasis.

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Screening of immunocompromised patients at risk of strongyloidiasis in western Turkey using ELISA and real-time PCR

Cited by 5 publications

References 17 publications

Describing universal Strongyloides serologic screening among pediatric intestinal and liver transplant recipients

Describing universal Strongyloides serologic screening among pediatric intestinal and liver transplant recipients

Strongyloides, cryptosporidium, and other parasitic infections

Strongyloides stercoralis in Patients on Corticosteroids Therapy Using Enzyme-Linked Immunosorbent Assay and Gelatin Particles Indirect Agglutination Tests: A Diagnostic Approach

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