Screening of In-Vitro Anti-Inflammatory and Antioxidant Activity of Sargassum ilicifolium Crude Lipid Extracts from Different Coastal Areas in Indonesia

Abstract

Sargassum brown seaweed is reported to exhibit several biological activities which promote human health, such as anticancer, antimicrobial, antidiabetic, anti-inflammatory, and antioxidant activity. This study aimed to investigate the anti-inflammatory and antioxidant activity of crude lipid extracts of Sargassum ilicifolium obtained from four different coastal areas in Indonesia, namely Awur Bay–Jepara (AB), Pari Island–Seribu Islands (PI), Sayang Heulang Beach–Garut (SHB), and Ujung Genteng Beach–Sukabumi (UGB). Results showed that treatment of RAW 264.7 macrophage cells with UGB and AB crude lipid extracts (12.5–50 µg/mL) significantly suppressed the nitric oxide production after lipopolysaccharide stimulation, both in pre-incubated and co-incubated cell culture model. The anti-inflammatory effect was most marked in the pre-incubated cell culture model. Both two crude lipid extracts showed 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and high ferric reducing antioxidant power, which were amounted to 36.93–37.87 µmol Trolox equivalent/g lipid extract and 681.58–969.81 µmol FeSO4/g lipid extract, respectively. From this study, we can conclude that crude lipid extract of tropical S. ilicifolium can be further developed as a source of anti-inflammatory and antioxidant agent.

Sargachromenol Purified from Sargassum horneri Inhibits Inflammatory Responses via Activation of Nrf2/HO-1 Signaling in LPS-Stimulated Macrophages

“…Recently, many researchers have reported several chromanol compounds of the terpenoids family, pigments, polyphenols, and sterols derived from Sargassum species and their anti-oxidant and anti-inflammatory effects [ 14 , 23 , 24 , 25 , 26 ]. Especially, recent studies have suggested the anti-inflammatory activities of the polysaccharides and loliolide derived from S. horneri , whereas there is no report on the biological effect of SC [ 16 , 17 ].…”

“…The activated macrophages cause inflammatory responses with the excessive production/expression of inflammatory cytokines, such as IL-1β, IL-6, and TNF-α, followed by tissue injury and multi-organ failure [ 17 , 23 ]. Thus, inhibitors of theses cytokines have been considered as candidates for anti-inflammatory materials.…”

Bibliometric Analysis of Marine Traditional Chinese Medicine in Pharmacopoeia of the People’s Republic of China: Development, Differences, and Trends Directions

“…Increased oxidative stress alters lipids, DNA, and proteins, leading to cellular inflammation and programmed cell death, which play an irreplaceable role in physiopathological conditions [ 98 ]. Sargassum ilicifolium crude lipid extracts demonstrated antioxidant activity in an in vitro experiment [ 99 ]. Macroalgae are also considered to be an important source of secondary metabolites and macromolecules with antioxidant activity [ 100 ].…”

A nanofiber hydrogel derived entirely from ocean biomass for wound healing

“…Materials and valuable applications beneficial to mankind can be obtained from these marine biological wastes [6], [7], [8], [9] . For example, collagen can be extracted from fish and other organisms, sodium alginate can be extracted from sea algae, and chitosan can be extracted from crustaceans [10], [11], [12] . Nowadays, there have been extensive studies on these raw materials because of their excellent biocompatibility and biodegradability [13] .…”

“…[6][7][8][9] For example, collagen can be extracted from sh and other organisms, sodium alginate can be extracted from sea algae, and chitosan can be extracted from crustaceans. [10][11][12] Recently, there have been extensive studies on these raw materials because of their excellent biocompatibility and biodegradability. 13 However, on the premise of reducing waste, there is still a lack of research on one-step green methods to extract and synthesize hydrogel excipients from marine waste.…”