Screening of melon populations for resistance to Didymella bryoniae in greenhouse and plastic tunnel conditions

Tsutsumi, Cláudio Yuji; Silva, Norberto da

doi:10.1590/s1516-89132004000200002

Cited by 12 publications

(10 citation statements)

References 4 publications

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“…Eldorado 300 was classified as susceptible by the reaction class (Table 3); however, the Scott-Knott clustering test grouped it as highly susceptible and not significantly different from Flexuosus 'M-2791', Melão verdadeiro, PI 224786, and JAB-20. Tsutsumi and Silva (2004) evaluated the 'Eldorado 300' in plastic tunnel and greenhouse, and suggested it as susceptible control, which was also observed in this study.…”

Section: Reaction Of Melon Genotypes To D Bryoniaesupporting

confidence: 74%

Reaction of melon genotypes to Didymella bryoniae (Fuckel) Rehm

Santos

Candido

Rabelo

et al. 2017

Chilean J. Agric. Res.

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Section: Reaction Of Melon Genotypes To D Bryoniaesupporting

confidence: 74%

Reaction of melon genotypes to Didymella bryoniae (Fuckel) Rehm

Santos

Candido

Rabelo

et al. 2017

Chilean J. Agric. Res.

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“…Next, signs of disease appear in leaves, vines, fruits and even other organs of the pumpkin. GSB can be transmitted rapidly from a local initial infection source to infect a large surrounding area, resulting in serious decreases in both pumpkin yield and quality (Tsutsumi & Silva, ). Considerable efforts have been expended to increase our understanding of the mechanisms underlying Sc .…”

Section: Introductionmentioning

confidence: 99%

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Zhao

Zhang

et al. 2018

Journal of Phytopathology

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The parasitic pathogenic fungus Stagonosporopsis cucurbitacearum (Sc.) is the main cause of pumpkin gummy stem blight (GSB), one of the most devastating pumpkin crop diseases in north‐east China. In the present study, transcriptome analyses of the GSB‐susceptible strain commonly grown in that region, zq‐1, were performed to identify differentially expressed genes (DEGs) relevant to GSB infection. Using RNA‐seq of six fungal samples, we obtained 44.62 Gb of clean sequence data with a Q30 base percentage of 92.38% or higher. We carried out functional annotation by comparing sequences against NR, Swiss‐Prot, KEGG, COG, KOG, GO, and Pfam databases and obtained annotation results for 38,110 unigenes. From these unigenes, a total of 341 DEGs were identified and subjected to GO functional annotation and KEGG pathway analysis. Subsequently, 52 genes possessed putative pathogenic functions, with 34 genes assigned to molecular function (including hydrolase, protein kinase, phosphatase, cellulase, etc.), 15 genes assigned to biological process, and 3 genes assigned to mitogen‐activated protein kinase pathways, ABC transport proteins and cellular endocytosis. Identification of DEGs should enhance our understanding of pathogenic genes involved in pumpkin infection by Sc. and should facilitate the systematic investigation of pathogenic gene expression patterns and host–pathogen interactions.

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“…Cucurbita maxima (pumpkin) is an annual herbaceous vine and a typical cucurbit that is susceptible to gummy stem blight (GSB). GSB is a severe and widespread disease that has caused greater losses to pumpkin production worldwide than any other diseases (Tsutsumi & Silva, ). In particular, this disease is most devastating in protected areas, such as on continuously cropped land, in plastic sheds and in greenhouses, where the incidence of C. maxima GSB can be as high as 15%–50% (Vaghefi, Hay, Pethybridge, Ford, & Taylor, ).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of Cucurbita gummy stem blight fungi in Northeast China

Zhao

Wu²,

Zhang

et al. 2018

Journal of Phytopathology

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Cucurbita maxima (pumpkin) is a typical cucurbit that is susceptible to gummy stem blight. In recent years, this major fungal disease has decimated pumpkin yields in Northeast China (Heilongjiang Province) in increasingly numerous outbreaks with more rapid spread in recent years. After culturing fungi on potato dextrose agar medium, we conducted a systematic study of the growth and morphological characteristics of various purified strains of Cucurbita gummy stem blight (GSB) fungus from across Northeast China. Subsequently, DNA samples of 30 isolates with distinct hyphal variations were subjected to internal transcribed spacer (ITS) sequencing. Sequence analysis resulted in identification of the isolates as Stagonosporopsis cucurbitacearum and demonstrated that this is a dominant and widely distributed fungal species in this region. Subsequently, multi‐site phylogenetic analysis assigned the 30 aforementioned strains to two genotypes that aligned to seven phenotypic types of Cucurbita GSB fungi. By analysing ITS conserved sequences of these phenotypically diverse groups, we found that Cucurbita GSB pathogens broadly shared two motifs that contained sequence variations unique to two groups in addition to common identical motifs. Ultimately, this study provided useful data for rapid and accurate identification of S. cucurbitacearum and diagnosis in early symptoms of Cucurbita GSB. This work also provides tools to explore the distribution and regularity of GSB outbreaks spatially and temporally across Northeast China.

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Screening of melon populations for resistance to Didymella bryoniae in greenhouse and plastic tunnel conditions

Abstract: ABSTRACT

Cited by 12 publications

References 4 publications

Reaction of melon genotypes to Didymella bryoniae (Fuckel) Rehm

Reaction of melon genotypes to Didymella bryoniae (Fuckel) Rehm

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Identification and characterization of Cucurbita gummy stem blight fungi in Northeast China

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