Screening of microbial contamination and antimicrobial activity of sea cucumber Holothuria polii

Abstract

Microbiological studies were carried out on microbial contamination and antimicrobial activity of sea cucumber Holothuria polii collected from Mediterranean Sea at Abu-kir shore of Alexandria, Egypt. The obtained results revealed the presence of isolates of five human Gram-negative pathogenic bacteria, representing five genera were identified to species level, including, Esherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella sp. and Shigella sp. In addition, an yeast Candida albicans was isolated. The pathogenic bacteria were identified using API 20E strip system (BioMereux). All collected H. polii specimens were healthy with no external signs of infection. Histopathological study of the tegument, intestine and gonads showed no abnormal changes. The antimicrobial activity of two tegumental ethanol extracts (A and B, differ in the method of dehydration) were tested against wide range of pathogenic bacteria and fungi, including intestinal, skin and nosocomial pathogens and one plant fungal pathogen. The results revealed a remarkable antifungal activity of the extract B at 2.5 mg/ml MIC90, especially on Aspergillus niger, Scloretium sp, C. albicans, Aspergillus flavus and Malassezia furfur, and limited antibacterial activity against Gram-negative bacteria (Salmonella choleraesuis ATCC 14028 and Aeromonas hydrophila). The domain of bacterial and limited fungal contamination confirms the results that showed strong antifungal activity of investigated extract.

Diversity and bioactivity of fungi associated with the marine sea cucumber Holothuria poli : disclosing the strains potential for biomedical applications

“…Isolated fungal strains all belonged to the phylum Ascomycota, confirming it as dominant in the marine environment (Jones et al 2019). Our data show a significantly higher fungal diversity (47 species) compared to that reported from previous investigation on the same animal (Omran and Allam 2013) as well as to that reported from other species of sea cucumbers (Pivkin 2000). Omran and Allam (2013) reported only a single isolate, C. albicans, associated with H. poli from the Egyptian Mediterranean Sea, a species not detected in our study.…”

Nutritional value of some Egyptian sea cucumbers

“…Also, it has been reported that holothurin is used in the treatment of asthma, gastric ulcer and high blood pressure (Ridzwan et al, 1990;Kaswandi et al, 1993;Hasan et al, 1996). Moreover, it has antiparasitic and a broad spectrum antifungal effect (Shimada, 1969;Kaswandi et al, 1999;Mona et al, 2012;Omran and Allam, 2012).…”

ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA

“…In contrast (Kabara, 1978) 705 found that the S. japonicus extract has no activity against gram positive and negative bacteria. As well as (Omran and Allam, 2012) showed that the tegument ethanol exctract of Holothuria leucospilata, H. polii, Bohadschia vitiensis and Actinopyga mauri-tania had no antibacterial effects against E. coli (gram negative) and B. subtilus (gram positive). Those variable findings showed that the activity of the extract may be changed according to the method of the extraction (Omran and Allam, 2012).…”

“…(Pringgenies and Apriliyani, 2012), sea cucumber Holothuria leucospilota (Pringgenies et al, 2014), Holothuria impatiens (Pringgenies et al, 2015). Two unsaturated fatty acids with potent α-Glusidase inhibitory activity had been purified from the body wall of sea cucumber Stichopus japonicus (Omran and Allam, 2012;McLafferty, 1980). Methanolic extract of Sticopus badionotus showed antibacterial effects against S aureus (Mc-Lafferty, 1980).…”