Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells

Wu, Fengpeng; Wu, Bingyue; Zhang, Xiaoxiao; Yang, Congrong; Zhou, Chenguang; Ren, Shengxiang; Wang, Jun; Yang, Yanming; Wang, Guiying

doi:10.3389/fonc.2021.699475

Cited by 5 publications

(4 citation statements)

References 89 publications

(91 reference statements)

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“…MiRNAs suppress the translation of mRNA and involve the posttranscriptional regulation. [ 27,28 ] Here, we ascertained that TRIM59 was a miR‐496 target. TRIM59 belongs to the TRIM protein family, which implicates in a series of cellular processes.…”

Section: Discussionmentioning

confidence: 99%

Circ_0006324 regulates cell proliferation, cell‐cycle progression, apoptosis, and glycolysis of non‐small cell lung cancer cells through miR‐496/TRIM59 axis

Wang,

Zhu,

Wang

et al. 2023

J Biochem & Molecular Tox

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Increasing evidence suggests that circular RNA (circRNA) plays an important role in non‐small cell lung cancer (NSCLC) progression. This study aimed to investigate the role and potential molecular mechanism of circ_0006324 in NSCLC. The expression levels of circ_0006324, miR‐496, miR‐488‐5p, and tripartite motif‐containing 59 (TRIM59) mRNA were determined by quantitative real‐time polymerase chain reaction (PCR). 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide assay, EdU assay, and flow cytometry were carried out to evaluate cell proliferation and apoptosis. The extracellular acidification rate and lactic acid production were examined to assess cell glycolysis. Western blot assay was used to detect protein levels. The target relationship of circ_0006324/miR‐496/TRIM59 axis was validated by RNA pull‐down assay, dual luciferase reporter assay, and radio immunoprecipitation assay. Xenograft tumor assay was performed to reveal the function of circ_0006324 in vivo. Circ_0006324 was upregulated in NSCLC and related to tumor node metastasis stage and distant metastasis. Knockdown of circ_00006324 impeded NSCLC cell proliferation, glycolysis, and promoted cell apoptosis. MiR‐496 was verified as a target of circ_0006324 and circ_00006324 mediated the altering of cell proliferation, apoptosis, and glycolysis of NSCLC cells through targeting miR‐496. TRIM59 was verified as a target of miR‐496, and circ_0006324 positively regulated TRIM59 expression by targeting miR‐496. Overexpression of TRIM59 could reverse the effects of circ_0006324 silencing on the proliferation, apoptosis, and glycolysis of NSCLC cells. Circ_0006324 knockdown impeded NSCLC tumor growth in vivo. Circ_0006324 functioned as a tumor promoter in NSCLC to promote cell proliferation, cell cycle progression, and glycolysis and inhibit cell apoptosis via miR‐496/TRIM59 axis.

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Section: Discussionmentioning

confidence: 99%

Circ_0006324 regulates cell proliferation, cell‐cycle progression, apoptosis, and glycolysis of non‐small cell lung cancer cells through miR‐496/TRIM59 axis

Wang,

Zhu,

Wang

et al. 2023

J Biochem & Molecular Tox

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“…Some of the biomarkers explored to date include the presence of mutations to assess the response to specific treatments, as mentioned earlier. Additionally, there are groups that have explored the expression of miRNA and found a relationship with resistance to RT[ 34 ]. Other studies have examined the expression of specific genes and established a prognostic risk score model to predict the response to ChT[ 35 ].…”

Section: Neoadjuvant Treatmentmentioning

confidence: 99%

Neoadjuvant treatment of rectal cancer: Where we are and where we are going

González Del Portillo,

Couñago,

López-Campos

2024

World J Clin Oncol

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Locally advanced rectal cancer requires a multidisciplinary approach based on total neoadjuvant treatment with radiotherapy (RT) and chemotherapy (ChT), followed by deferred surgery. Currently, alternatives to the standard total neoadjuvant therapy (TNT) are being explored, such as new ChT regimens or the introduction of immunotherapy. With standard TNT, up to a third of patients may achieve a complete pathological response (CPR), potentially avoiding surgery. However, as of now, we lack predictive markers of response that would allow us to define criteria for a conservative organ strategy. The presence of mutations, genes, or new imaging tests is helping to define these criteria. An example of this is the diffusion coefficient in the diffusion-weighted sequence of magnetic resonance imaging and the integration of this imaging technique into RT treatment. This allows for the monitoring of the evolution of this coefficient over successive RT sessions, helping to determine which patients will achieve CPR or those who may require intensification of neoadjuvant therapy.

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“…Likewise, in colorectal cancer, microRNA-96 targets the tumor proteins fork head box protein O1 (FOXO1) and FOXO3a, that stimulates the growth of cancer cells ( Gao & Wang, 2015). Furthermore, one cause of the redaction resistance of rectal cancer cells may be decreased level of expression of the GPC3 gene, which is directly regulated by miRNA-96-5p (Wu et al, 2021). This impact may be connected to alterations in the activity of the Wnt/-catenin signal transduction pathway.…”

Section: Cancer and Mirna-96 Expressionmentioning

confidence: 99%

A New Insight of MicroRNA-96 In Human Malignant Disorders and Drug Resistance

2023

QZJ

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Autumn2023miRNA-96 is a short non-coding RNA molecule which plays an essential function in the regulation of post-transcriptional gene, and play a role in the development of a number of illnesses, such as cancer, depending on the cellular setting, it may function as a tumor suppressor or an oncogene. According to a recent research, miRNA-96 levels has been decreased in gastric cancer, breast cancer, pancreatic cancer, renal cancer and cervical cancer. However, the levels of miRNA-96 in several kinds of cancers are increased, including colorectal, lung, prostate, glioma, osteosarcoma, and hepatocellular carcinoma. The current review aims to offer a summary of miRNA-96's role in the advancement of cancer illnesses and with an emphasis on dysregulated signaling pathways. Based on in vivo, in vitro, and human research, we also go over the function of this miRNA-96 as a cancer biomarker of prognosis and emphasize how it contributes to drug resistance.

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Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells

Cited by 5 publications

References 89 publications

Circ_0006324 regulates cell proliferation, cell‐cycle progression, apoptosis, and glycolysis of non‐small cell lung cancer cells through miR‐496/TRIM59 axis

Circ_0006324 regulates cell proliferation, cell‐cycle progression, apoptosis, and glycolysis of non‐small cell lung cancer cells through miR‐496/TRIM59 axis

Neoadjuvant treatment of rectal cancer: Where we are and where we are going

A New Insight of MicroRNA-96 In Human Malignant Disorders and Drug Resistance

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