Screening of monoterpene indole alkaloids in six <i>Rauwolfia</i> species by ultra‐high performance liquid chromatography orbitrap velos pro mass spectrometer

Kumar, Sunil; Singh, Awantika; Kumar, Brijesh

doi:10.1002/sscp.201900029

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Mass spectrometric analysis was performed on an Agilent 6520 QTOF mass spectrometer in positive ESI mode, as reported by Bajpai et al [22]. Appropriate measurements were taken using the auto mass calibration method with an external mass calibration solution, as reported by Kumar et al [23].…”

Section: Mass Spectrometrymentioning

confidence: 99%

Determination of UPLC-ESI MS/MS- and GC-MS-based altitudinal variations in the bioactive potential of traditional medicinal plants

Singh,

Passari,

Kumar

et al. 2024

Preprint

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Traditional medicinal plants have attracted scientific interest due to their bioactive compounds, and the levels of their constituents vary with location and altitude. The present study was designed to evaluate the pharmacological potential of two traditional medicinal plants, Mikania micrantha and Ageratum huostonianum; these were collected from two sites, Murlen National Park (MNP) and Dampa Tiger Reserve (DTR), which are located at different altitudes. Both plant species are used by local traditional healers in Mizoram, Northeast India, for the treatment of various health problems. We hypothesized that altitudinal variation would affect these plants' chemical composition and bioactive potential. Plant extracts were evaluated for antioxidant, antimicrobial, and cytotoxic activities. The results show that the plants located at a higher altitude, i.e., MNP, showed higher TPC (615.7 ± 0.58 and 453.80 ± 0.95 µg gallic acid equivalents/mg of plant extract dry weight (µg GAE/mg) for M. micrantha and A. huostonium, respectively) and TFC (135.4 ± 0.46 and 120.66 ± 1.93 µg quercetin equivalents/mg of plant extract dry weight (µg GE/mg) for M. micrantha and A. huostonium, respectively). The extract of A. houstonianum (MNP) exhibited significantly greater antioxidant activity against ABTS radicals (IC50 241.6 µg/mL) as compared to the extract of A. houstonianum (DTR) (IC50 371.2 µg/mL). The composition of the bioactive compounds present in the plants was determined using UPLC-ESI MS/MS and GC/MS, which detected five and ten compounds in the A. houstonianum and M. micrantha extracts, respectively. Plant species collected from the Murlen National Park site had high bioactivity potential and contained several bioactive compounds. A distinct variation between the volatile and non-volatile compounds was revealed. The collective data in this study show the influence of altitude on the biological compound production of selected medicinal plants. The findings will be utilized in determining the plant material needed for the development of bioactive formulations.

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Section: Mass Spectrometrymentioning

confidence: 99%

Determination of UPLC-ESI MS/MS- and GC-MS-based altitudinal variations in the bioactive potential of traditional medicinal plants

Singh,

Passari,

Kumar

et al. 2024

Preprint

Self Cite

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show abstract

“…Many methods for the analysis of indole alkaloids have been developed and high-performance liquid chromatography (HPLC) is one of the most widely used methods [ 12 , 13 ]. However, due to the interaction between the basic nitrogen atom in the alkaloid structure and the un-bonded acidic silanol group of the stationary phase, this method has shortcomings, such as the broadening, tailing, and drifting of chromatographic peaks.…”

Section: Introductionmentioning

confidence: 99%

Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures

You

Wang

et al. 2021

Molecules

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Diffusion-ordered spectroscopy (DOSY) is a powerful tool for investigating mixtures and identifying peaks of chemical components. However, similar diffusion coefficients of the components, particularly for complex mixtures that contain crowded resonances, limit resolution and restrict application of the DOSY technique. In this paper, matrix-assisted DOSY were used to explore whether the diffusion resolution of a complex model involving indole alkaloid mixtures can be realized. Furthermore, we investigated the influence of different factors on the separation effect. The results showed that the changes in diffusion coefficient differences were achieved more obviously when using sodium dodecyl sulfate (SDS) micelles as the matrix. In addition, we also found that increasing the concentration of SDS can improve the resolution of the DOSY spectrum. Finally, after investigating the influence factors and NMR conditions, we demonstrated the applications of the SDS-assisted DOSY on analyzing the total alkaloid extract of Alstonia Mairei, and the virtual separation of mixtures was achieved.

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Phytochemistry and Pharmacology of Catharanthus roseus (L.) G. Don and Rauvolfia serpentina (L.) Benth. ex Kurz

Kumar,

Singh

2023

Bioprospecting of Tropical Medicinal Plants

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Screening of monoterpene indole alkaloids in six Rauwolfia species by ultra‐high performance liquid chromatography orbitrap velos pro mass spectrometer

Cited by 6 publications

References 23 publications

Determination of UPLC-ESI MS/MS- and GC-MS-based altitudinal variations in the bioactive potential of traditional medicinal plants

Determination of UPLC-ESI MS/MS- and GC-MS-based altitudinal variations in the bioactive potential of traditional medicinal plants

Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures

Phytochemistry and Pharmacology of Catharanthus roseus (L.) G. Don and Rauvolfia serpentina (L.) Benth. ex Kurz

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