Screening of plant growth-promoting bacteria isolated from sugarcane

Bezerra, Gabrielle Alves; Takita, Marco Aurélio; Tosta, Christiann Davis; Ceccato-Antonini, Sandra Regina; Rosa-Magri, Márcia Maria

doi:10.5433/1679-0359.2022v43n4p1757

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…Disposable loops were used to transfer bacteria to agar, which was then spread using cotton swaps then left for a full day at 37 degrees Celsius to allow the bacteria to become active. [20]. Following the activation of the bacteria, they were put in 3 ml of normal saline, and the turbidity of the system was calibrated using a spectrophotometer to a standard of 0.5 mcfarland.…”

Section: Antibacterial Testsmentioning

confidence: 99%

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Alhazza¹,

Al-Lami²,

AlShaheen³

et al. 2022

Journal of Pharmaceutical Negative Results

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Multidrug resistance has resulted in hurtful infections on treatment with common antimicrobials and is associated with health threatening problems. Antibiotic abuse in health care systems contributes to global resistance. β-lactams are the predominantly used antibiotics, and the most often used is cefotaxime, therefore facing a resistance problem. Potent cephalosporin derivatives, β-lactamase inhibitors, prodrugs, and drug nano-carriers are approaches to cutback bacterial resistance. An example of a nanocarrier applied in field of antibacterial activity enhancement is niosomes. Niosomes are self-assembled vesicles made from non-ionic surfactants with cholesterol also additives included or omitted that can provide good features such as antibiotic shielding, cleavage proofing, controlled release, and specific targeting. Cefotaxime niosomes preparation done by Thin-Film Hydration method using Tween 40, span 60, stearylamine, and cholesterol. The dried film was moisturised with buffered solution of cefotaxime at 45-55°C then the final nanosized niosomal cefotaxime was generated through a probe sonication. A 27 formulas were prepared and identified by dynamic light scattering so that polydispersity index and the vesicular size are measured. The selected formula of cefotaxime niosomes (CN6) has as showed the lowermost values and a prominent vesicular structure of 106 nm diameter on Transmission electron microscope and compatibility on Fourier Transform Infrared System analysis. CN6 remained unchanged in diameter and Poly-dispersity index after 100 days of storage at 2 to-80 C and also onsubsequent freeze drying and reconstitution. Entrapment efficiency was quantified by the ultracentrifugation method and was discovered to be 93 %. Antibiotic delivery by cefotaxime-eluting niosomes has been shown to be successful with antibacterial potentiation of 160, 8, 640 and 40 times better than aqueous solution against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa respectively which is obviously a considerable drop in the minimum inhibitory concentrations of the selected formula versus the aqueous cefotaxime solution and this was evaluated using Agar well diffusion technique.

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Section: Antibacterial Testsmentioning

confidence: 99%

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Alhazza¹,

Al-Lami²,

AlShaheen³

et al. 2022

Journal of Pharmaceutical Negative Results

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Soy Molasses as Culture Medium for Bacillus Species Aiming at Plant Growth Promotion

Correa da Silva,

Dorigan,

da Silva-Neto

et al. 2024

Fermentation

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Soy molasses, a by-product from the processing of soy protein concentrate, is a low-cost feedstock for fermentation processes due to its high content of fermentable sugars. This work investigates the use of soy molasses for growing Bacillus species, aiming at their potential application as plant growth promoters. Firstly, six Bacillus strains were screened for their ability to grow in increasing concentrations of soy molasses in a microplate assay. Following this, shaken-flask assays for growth and γ-polyglutamic acid (γ-PGA) production by three Bacillus strains in medium E and soy molasses media with 28 and 56 g L−1 of total reducing sugars (TRS) were carried out. An in vivo experiment evaluated the effect of the bacterial fermented broths on the germination and initial development of maize. Soy molasses supported the growth of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus licheniformis in concentrations of 28 and 56 g L−1 TRS, but it was inhibitory at 112 and 224 g L−1 TRS. In soy molasses media, growth was not always associated with γ-PGA production, which was a maximum of 56 g L−1 TRS for B. amyloliquefaciens and B. licheniformis. Fermented broths with B. subtilis and B. licheniformis in soy molasses media (56 and 28 g L−1 TRS, respectively) applied to maize seeds resulted in the highest Vigor Indexes of the seedlings, which correlated negatively with the broth pH and were not impacted by the γ-PGA and indole acetic acid produced by the bacteria. The low-cost and easily available feedstock soy molasses constitutes a potential culture medium for the growth of plant growth-promoting bacteria.

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Screening of plant growth-promoting bacteria isolated from sugarcane

Cited by 2 publications

References 38 publications

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Cefotaxime Eluting Niosomes as A Novel Approach to Potentiate the Antibacterial Activity

Soy Molasses as Culture Medium for Bacillus Species Aiming at Plant Growth Promotion

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