Screening of Polyphenols in Tobacco (Nicotiana tabacum) and Determination of Their Antioxidant Activity in Different Tobacco Varieties

Abstract

Tobacco (Nicotiana tabacum) is an herbaceous plant originating from South America and processed into cigarettes for consumption. Polyphenols are considered vital components of tobacco in view of their contribution to antioxidant properties. This study aimed to determine the phenolic compounds in different tobacco varieties by applying cold extraction with methanol and distilled water. The extracts were screened for phenolic compound diversity and distribution as well as their antioxidant potential in different tobacco varieties. The results showed that the methanolic extract of tobacco SP-28 exhibited the highest value in the total phenolic content (24.82 ± 0.07 mg GAE/gd.w.) and total flavonoid content (4.42 ± 0.01 mg QE/gd.w.), while the water extract of tobacco SN-2 exhibited the highest value in the total condensed tannin (1.12 ± 0.03 mg CE/gd.w.). The radical scavenging capacities of tobacco SP-28 were relatively high in DPPH (18.20 ± 0.01 mg AAE/gd.w.) and FRAP (3.02 ± 0.10 mg AAE/gd.w.), whereas the ABTS value was the highest in tobacco SN-2 (37.25 ± 0.03 mg AAE/gd.w.), and the total antioxidant capacity was the highest in tobacco SN-1 (7.43 ± 0.18 mg AAE/gd.w.). LC-ESI-QTOF-MS/MS identified a total of 49 phenolic compounds, including phenolic acids (14), flavonoids (30), and other polyphenols (5) in four different tobacco varieties. Tobacco SP-28 showed the highest number of phenolic compounds, especially enriched in flavones. Our study highlights the antioxidant potential of tobacco extracts and reveals the phenolic distribution among different tobacco varieties that could support tobacco utilization in different pharmaceutical industries.

Phytochemical, Antioxidant, Antihyaluronidase, Antityrosinase, and Antimicrobial Properties of Nicotiana tabacum L. Leaf Extracts

“…Because phenols are highly soluble in polar solvents, they can provide higher concentrations of phenols than nonpolar solvents [ 37 ]. The amounts of phenolics found in ethanolic extracts in this study were significantly higher than previously reported by Zou et al, who reported that the methanolic extract of N. tabatum had the highest amount in terms of total phenolic content, at 24.82 ± 0.07 mg GAE/g extract [ 38 ]. However, different solvents and extraction methods were considered.…”

“…Wang et al reported that ethanolic-extracted polyphenols from tobacco leaf possessed strong antioxidant activity when measured for the radical scavenging and lipid peroxidation inhibition activity [14]. Similarly, a previous investigation revealed that N. tabacum extracts exhibited strong antioxidant activities, including radical scavenging activity against DPPH and ABTS, as well as ferric reducing power, due to their polyphenols [38]. With regard to different methods to evaluate the antioxidant activity of tobacco, it was found that tobacco presented antioxidant capacity in the range of 66 to 230 μmol Trolox equivalent/g dry weight using the ORAC method [45].…”

Determination and Characterization of Phenolic Compounds from Australia-Grown Sweet Cherries (Prunus avium L.) and Their Potential Antioxidant Properties

“…Determination of ABTS. The ABTS radicalscavenging activity of the samples was measured using the method of Peng et al 19 and Zou et al 66 The ABTS + solution was prepared by the addition of 7 mM ABTS solution and 140 mM potassium persulfate solution and incubated in the dark for 16 h. The ABTS + solution was diluted with ethanol to make an absorbance of 0.70 ± 0.02 at 734 nm. The extract (10 μL) and 290 μL of freshly prepared ABTS + solution were added to the 96-well plate and incubated at 25 °C for 6 min.…”

LC-ESI-QTOF-MS/MS Characterization and Estimation of the Antioxidant Potential of Phenolic Compounds from Different Parts of the Lotus (Nelumbo nucifera) Seed and Rhizome

“…The phenolic contents are highly associated with their antioxidant properties. − Thus, several antioxidant assays were conducted to analyze the antioxidant capacity of the lotus samples. In our study, DPPH, ABTS, FRAP, and TAC were applied to estimate the antioxidant potential of different parts of the lotus.…”